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The map label for this gene is purF [H]

Identifier: 162149273

GI number: 162149273

Start: 3604281

End: 3605834

Strand: Direct

Name: purF [H]

Synonym: GDI_3505

Alternate gene names: 162149273

Gene position: 3604281-3605834 (Clockwise)

Preceding gene: 162149272

Following gene: 162149274

Centisome position: 91.38

GC content: 68.6

Gene sequence:

>1554_bases
ATGTCGACCCGCCCCACCGCCCCGCAGGCCGTCACCCCGGACAGCGCCGCCTGCTCCTGCCCCCTGTGCGCCACCGGCCA
CGCGCGGGACGACATGCCGCTGCAATGGCATGACGACGACAAGCCGCACGAGGAATGCGGCGTCATCGGCGTCTGGAACG
TCAAGGACGCGTCGGCGCTGACCGCGCTGGGCCTGCACGCGCTGCAGCATCGCGGGCAGGAGGCGACGGGCATCGTGTCC
TATGACAGCACGCGCTTCCACACCCACAAGGGGCTGGGCCTGGTGGGCGACGTGTTCGGTGACGCCCGGGTCATGGCCAC
CCTGCCGGGCCACCGGGCGGTGGGCCACAACCGCTACGCCACCACCGGGGCGACGCTGATCCGCAACGTGCAGCCCCTGT
TCGCGGATTTCGAATTCGGCGGTCTGGCGGTCGCGCACAACGGCAACCTGACGAACGCCGAGACGCTGAAGCGCGCGCTG
GTGCGCCGGGGCTGCATCTTCCAGTCCACGACGGACAGCGAGGTGTTCATCCACCTGCTGGCGATCTCGCTCTACGCCAC
GGTGCTGGACCGGCTGATCGACGCGCTGAAGCAGGTGCTGGGGGCCTATTCGCTGGTCGTGCTGTCGCGCGACGCGCTGA
TGGGCGTGCGCGACCCGCTGGGCGTGCGGCCGCTGATCCTGGGCCGCATCCCCGGCGAGACCGACGGCGCCGCCCCCTCC
TGGGTCCTGGCCAGCGAGACCTGCGCGCTGGACATCATGGGCGCGGAATTCGTCCGCGACGTGGAACCGGGCGAAATCGT
CATCATCGACAATGACGGCGTCCGTTCGGTCAAGCCGTTCGGCGAGCGGAAATCCCGCTTCTGCGTGTTCGAATACATCT
ATTTCGCCCGCCCGGATTCGGTGATGGACGGCAAGCCGGTCTACGAGGCGCGCAAGCGCATCGGCGTGGAACTGGCGCGC
GAAAGCGCGGTCGAGGCCGACGTGATCGTCCCGGTACCCGATTCCGGCGTGCCCTCGGCCATGGGCTATTCCATGGCCAG
CGGCATTCCCTTCGAACTGGGCATCATCCGCAACCATTATGTCGGCCGCACCTTCATCGAACCGACCGACCAGATCCGCC
ATCTGGGCGTGAAGCTGAAACATTCGACCAACCGGCCGGTGCTGGACGGCAAGCGGGTGGTCCTGGTCGATGATTCGATC
GTGCGCGGCACCACGTCGCGCAAGATCGTGGACATGGTCCGCGCCGCCGGCGCGCGCGAGGTGCACATGCGCATCTCCTC
GCCGCCCACGACGCATTCCTGCTTCTACGGCATCGACACGCCCGAGCGCAGCAAGCTGCTGGCGGCCCAGCACGACCTGA
AGGAAATGGCCGAACTGATCGGCGTGGACAGCCTGGCCTTCATCTCCTTCGACGGGCTGTACCGCGCGCTGGGCCACGCC
GACCGCGCCGCCGCCGCCGGCCGGTACTGCGACGCCTGCTTCACCGGCGACTACCCGATCCCCCTGGTGGATTACGAAGC
CGCAGGCGACGACGCGCGTCCCGGCACTCCATGA

Upstream 100 bases:

>100_bases
ACGCGCCATGACGCCCCGATCTGACCCCTTCGCGGGGCCGCCTTGTGCCTGCCCGGGAAGCACCACCCGGGCGCCCGTCG
AGGAAAAGAGGTAGCTGACC

Downstream 100 bases:

>100_bases
CTCCAGCCACAGTGACCGGGGCCCCGGTGGCACTGGTCACCGGGGCCAGCCGGGGCATCGGCCGCGCCACCGCCCTGGCC
CTGGCCGCGGGCGGGGCGCA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSTRPTAPQAVTPDSAACSCPLCATGHARDDMPLQWHDDDKPHEECGVIGVWNVKDASALTALGLHALQHRGQEATGIVS
YDSTRFHTHKGLGLVGDVFGDARVMATLPGHRAVGHNRYATTGATLIRNVQPLFADFEFGGLAVAHNGNLTNAETLKRAL
VRRGCIFQSTTDSEVFIHLLAISLYATVLDRLIDALKQVLGAYSLVVLSRDALMGVRDPLGVRPLILGRIPGETDGAAPS
WVLASETCALDIMGAEFVRDVEPGEIVIIDNDGVRSVKPFGERKSRFCVFEYIYFARPDSVMDGKPVYEARKRIGVELAR
ESAVEADVIVPVPDSGVPSAMGYSMASGIPFELGIIRNHYVGRTFIEPTDQIRHLGVKLKHSTNRPVLDGKRVVLVDDSI
VRGTTSRKIVDMVRAAGAREVHMRISSPPTTHSCFYGIDTPERSKLLAAQHDLKEMAELIGVDSLAFISFDGLYRALGHA
DRAAAAGRYCDACFTGDYPIPLVDYEAAGDDARPGTP

Sequences:

>Translated_517_residues
MSTRPTAPQAVTPDSAACSCPLCATGHARDDMPLQWHDDDKPHEECGVIGVWNVKDASALTALGLHALQHRGQEATGIVS
YDSTRFHTHKGLGLVGDVFGDARVMATLPGHRAVGHNRYATTGATLIRNVQPLFADFEFGGLAVAHNGNLTNAETLKRAL
VRRGCIFQSTTDSEVFIHLLAISLYATVLDRLIDALKQVLGAYSLVVLSRDALMGVRDPLGVRPLILGRIPGETDGAAPS
WVLASETCALDIMGAEFVRDVEPGEIVIIDNDGVRSVKPFGERKSRFCVFEYIYFARPDSVMDGKPVYEARKRIGVELAR
ESAVEADVIVPVPDSGVPSAMGYSMASGIPFELGIIRNHYVGRTFIEPTDQIRHLGVKLKHSTNRPVLDGKRVVLVDDSI
VRGTTSRKIVDMVRAAGAREVHMRISSPPTTHSCFYGIDTPERSKLLAAQHDLKEMAELIGVDSLAFISFDGLYRALGHA
DRAAAAGRYCDACFTGDYPIPLVDYEAAGDDARPGTP
>Mature_516_residues
STRPTAPQAVTPDSAACSCPLCATGHARDDMPLQWHDDDKPHEECGVIGVWNVKDASALTALGLHALQHRGQEATGIVSY
DSTRFHTHKGLGLVGDVFGDARVMATLPGHRAVGHNRYATTGATLIRNVQPLFADFEFGGLAVAHNGNLTNAETLKRALV
RRGCIFQSTTDSEVFIHLLAISLYATVLDRLIDALKQVLGAYSLVVLSRDALMGVRDPLGVRPLILGRIPGETDGAAPSW
VLASETCALDIMGAEFVRDVEPGEIVIIDNDGVRSVKPFGERKSRFCVFEYIYFARPDSVMDGKPVYEARKRIGVELARE
SAVEADVIVPVPDSGVPSAMGYSMASGIPFELGIIRNHYVGRTFIEPTDQIRHLGVKLKHSTNRPVLDGKRVVLVDDSIV
RGTTSRKIVDMVRAAGAREVHMRISSPPTTHSCFYGIDTPERSKLLAAQHDLKEMAELIGVDSLAFISFDGLYRALGHAD
RAAAAGRYCDACFTGDYPIPLVDYEAAGDDARPGTP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=510, Percent_Identity=38.0392156862745, Blast_Score=353, Evalue=2e-97,
Organism=Escherichia coli, GI1788651, Length=451, Percent_Identity=37.6940133037694, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1790167, Length=241, Percent_Identity=30.7053941908714, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=38.6694386694387, Blast_Score=320, Evalue=8e-88,
Organism=Saccharomyces cerevisiae, GI6323958, Length=479, Percent_Identity=34.2379958246347, Blast_Score=246, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=41.1885245901639, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24659604, Length=467, Percent_Identity=41.9700214132762, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI28573187, Length=492, Percent_Identity=41.260162601626, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55952; Mature: 55821

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRPTAPQAVTPDSAACSCPLCATGHARDDMPLQWHDDDKPHEECGVIGVWNVKDASAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHCCEEEEECCCCHHHH
TALGLHALQHRGQEATGIVSYDSTRFHTHKGLGLVGDVFGDARVMATLPGHRAVGHNRYA
HHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCC
TTGATLIRNVQPLFADFEFGGLAVAHNGNLTNAETLKRALVRRGCIFQSTTDSEVFIHLL
HHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHH
AISLYATVLDRLIDALKQVLGAYSLVVLSRDALMGVRDPLGVRPLILGRIPGETDGAAPS
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHCCCCCCCCCCEEEECCCCCCCCCCCC
WVLASETCALDIMGAEFVRDVEPGEIVIIDNDGVRSVKPFGERKSRFCVFEYIYFARPDS
EEEECCCEEEEEECHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCC
VMDGKPVYEARKRIGVELARESAVEADVIVPVPDSGVPSAMGYSMASGIPFELGIIRNHY
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCHHHHCCCCEEEEEECCCC
VGRTFIEPTDQIRHLGVKLKHSTNRPVLDGKRVVLVDDSIVRGTTSRKIVDMVRAAGARE
CCCEECCCHHHHHHCCEEEECCCCCCCCCCCEEEEECCCHHCCCCHHHHHHHHHHCCCEE
VHMRISSPPTTHSCFYGIDTPERSKLLAAQHDLKEMAELIGVDSLAFISFDGLYRALGHA
EEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCH
DRAAAAGRYCDACFTGDYPIPLVDYEAAGDDARPGTP
HHHHHCCCCCCEEECCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
STRPTAPQAVTPDSAACSCPLCATGHARDDMPLQWHDDDKPHEECGVIGVWNVKDASAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHCCEEEEECCCCHHHH
TALGLHALQHRGQEATGIVSYDSTRFHTHKGLGLVGDVFGDARVMATLPGHRAVGHNRYA
HHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCC
TTGATLIRNVQPLFADFEFGGLAVAHNGNLTNAETLKRALVRRGCIFQSTTDSEVFIHLL
HHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHH
AISLYATVLDRLIDALKQVLGAYSLVVLSRDALMGVRDPLGVRPLILGRIPGETDGAAPS
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHCCCCCCCCCCEEEECCCCCCCCCCCC
WVLASETCALDIMGAEFVRDVEPGEIVIIDNDGVRSVKPFGERKSRFCVFEYIYFARPDS
EEEECCCEEEEEECHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCC
VMDGKPVYEARKRIGVELARESAVEADVIVPVPDSGVPSAMGYSMASGIPFELGIIRNHY
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCHHHHCCCCEEEEEECCCC
VGRTFIEPTDQIRHLGVKLKHSTNRPVLDGKRVVLVDDSIVRGTTSRKIVDMVRAAGARE
CCCEECCCHHHHHHCCEEEECCCCCCCCCCCEEEEECCCHHCCCCHHHHHHHHHHCCCEE
VHMRISSPPTTHSCFYGIDTPERSKLLAAQHDLKEMAELIGVDSLAFISFDGLYRALGHA
EEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCH
DRAAAAGRYCDACFTGDYPIPLVDYEAAGDDARPGTP
HHHHHCCCCCCEEECCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA