Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 162149138

GI number: 162149138

Start: 3470690

End: 3472240

Strand: Direct

Name: oprM [H]

Synonym: GDI_3368

Alternate gene names: 162149138

Gene position: 3470690-3472240 (Clockwise)

Preceding gene: 162149137

Following gene: 162149141

Centisome position: 88.0

GC content: 67.18

Gene sequence:

>1551_bases
ATGACTTCGCCATTTTCCCTGATGTCCCGCCGGGGCGCCCTGCATCTGGGCGGCATTCTGGCACCGCTGGCGCTGGCCGG
CTGCATGGTCGGGCCATCCTACCACCGCCCCGCCGCCCCGGTTTCGGCCCGCTTCAAGGAACTGACCCCGGCCGCAGGCT
GGGAACGCGCCCGGCCCGCCATGGCCGAACTGCCCAAGGGCGCCTGGTGGACGATCTATAACGATCCCGTCCTGAACCGA
CTGGAATCGCAGGTCGCGATCTCGAACCAGAACGTGCGGATGTACGAGGCCAACTACCGCCAGGCCCGCGCCATGATCGA
CAGCGTGCGGGCACAGCTTTTTCCCACCCTCAGCGGCAGCCTGGGGTTCAACCGCAACAGCCAGGGGCGTGGCTCGCGCT
CGGCGTCCACCGGCAGCCTGGTCAGCTACGGGGGGGCGGCGACCAACACGACCGAGACGACCTATTCGATGGGCCCCAGC
GCAAGCTGGGACCTGGATCTGTGGGGCCGCATCCGCCGCAATATCCAAAGCCAGGTGACCGAGGCCCAGGCCAGCGCCGC
CGACCTGGCGAATGCCACCCTGTCCTACCAGGCGCAGCTCGCCACGGCCTATTTCAACCTGCGCTACCAGGATTCGCTGC
AGGAGCTGCTGCGCCGCTACGTGGATTTCAACACCCAGGCGCTGCAGATCACGCAGAACCAGTACGAGGCCGGCACCGCC
GATCCGACGGCGGTGATGCAGGCCCGCACCCAGCTTGAACAGAATCGTGCCACGCTGATCCAGGCCGGCATCGCCCGCGC
GCAGTACGAACACGCCATCGCGGTGCTGATGGGCCGTCCGCCCGCCGACCTGACGATCACGCCCGGCACGCTGCCCCGCC
AGATCCCCGCCATTCCGGTCGGGGTCCCCGCCGACCTGCTGCAGCGCCGCCCCGACGTGGCCGCCGCCGAACGGGCCATG
GAACAGTACAACGCCCAGATCGGCGCCGACATGGCGGCGTTCTTCCCCGACGTCACGCTGACGGCCGACTATTCCTACAG
CGGCGACCCCATCGGGCAACTGGTCCAGGTGGTCAACCGGATCTGGTCGCTCGGCGCCTCGGCCTCGGAAGTCCTGTTCC
AGGGCGGGTCCCGCATGGCGGCGGTGCACGGCGCCAACGCCCAGTACGACAGCGCCGTCGCGACCTACCGCCAGACCGTG
CTGACGGCCCTGCAGAATACCGAGGACAATCTGTCCAACCTGCGCATCCTTGAACAGCAGGCAACCCAGCAGCAGATCGC
CCTGGATTTCGCCAACCAGGCCGTCCAGGTCTCACTGAACCAGTACGAGGCCGGAACCCAGATCTACACCACGGTCATCA
CCAACGAGACCACGGCCCTCAGCAATGCCGAATCCGCCCTGTCCATCCAGCAGCAGCGCATGGTCGATTCCGTGGCGCTG
GTGCAGGCGCTGGGCGGCGGGTGGAACGCCACCAGCCTGCCGTCGAAGGCTTCGATGCAGACCGACAATCCGTTCCTGCC
CTCCTTTATCCAGAAGGACAAAAACCAGTAG

Upstream 100 bases:

>100_bases
TCGACCGCTTCGGCAGGTGGTGCCGGGGCCGATACCTCTCCGCCGCCCGCGCCCTGACGGGCAGGCGCGATCCACTCTCC
CAACAGGATGCCTGAACCGG

Downstream 100 bases:

>100_bases
GGCCGGAACCGGCGATGCCCGAACCGGCTGGGGCGATCACAGGATCGCCTTGGCCGTCAGCAGCGCGATCAGCAGGAAGA
TGACGAAGGCCAGGATGAAG

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTSPFSLMSRRGALHLGGILAPLALAGCMVGPSYHRPAAPVSARFKELTPAAGWERARPAMAELPKGAWWTIYNDPVLNR
LESQVAISNQNVRMYEANYRQARAMIDSVRAQLFPTLSGSLGFNRNSQGRGSRSASTGSLVSYGGAATNTTETTYSMGPS
ASWDLDLWGRIRRNIQSQVTEAQASAADLANATLSYQAQLATAYFNLRYQDSLQELLRRYVDFNTQALQITQNQYEAGTA
DPTAVMQARTQLEQNRATLIQAGIARAQYEHAIAVLMGRPPADLTITPGTLPRQIPAIPVGVPADLLQRRPDVAAAERAM
EQYNAQIGADMAAFFPDVTLTADYSYSGDPIGQLVQVVNRIWSLGASASEVLFQGGSRMAAVHGANAQYDSAVATYRQTV
LTALQNTEDNLSNLRILEQQATQQQIALDFANQAVQVSLNQYEAGTQIYTTVITNETTALSNAESALSIQQQRMVDSVAL
VQALGGGWNATSLPSKASMQTDNPFLPSFIQKDKNQ

Sequences:

>Translated_516_residues
MTSPFSLMSRRGALHLGGILAPLALAGCMVGPSYHRPAAPVSARFKELTPAAGWERARPAMAELPKGAWWTIYNDPVLNR
LESQVAISNQNVRMYEANYRQARAMIDSVRAQLFPTLSGSLGFNRNSQGRGSRSASTGSLVSYGGAATNTTETTYSMGPS
ASWDLDLWGRIRRNIQSQVTEAQASAADLANATLSYQAQLATAYFNLRYQDSLQELLRRYVDFNTQALQITQNQYEAGTA
DPTAVMQARTQLEQNRATLIQAGIARAQYEHAIAVLMGRPPADLTITPGTLPRQIPAIPVGVPADLLQRRPDVAAAERAM
EQYNAQIGADMAAFFPDVTLTADYSYSGDPIGQLVQVVNRIWSLGASASEVLFQGGSRMAAVHGANAQYDSAVATYRQTV
LTALQNTEDNLSNLRILEQQATQQQIALDFANQAVQVSLNQYEAGTQIYTTVITNETTALSNAESALSIQQQRMVDSVAL
VQALGGGWNATSLPSKASMQTDNPFLPSFIQKDKNQ
>Mature_515_residues
TSPFSLMSRRGALHLGGILAPLALAGCMVGPSYHRPAAPVSARFKELTPAAGWERARPAMAELPKGAWWTIYNDPVLNRL
ESQVAISNQNVRMYEANYRQARAMIDSVRAQLFPTLSGSLGFNRNSQGRGSRSASTGSLVSYGGAATNTTETTYSMGPSA
SWDLDLWGRIRRNIQSQVTEAQASAADLANATLSYQAQLATAYFNLRYQDSLQELLRRYVDFNTQALQITQNQYEAGTAD
PTAVMQARTQLEQNRATLIQAGIARAQYEHAIAVLMGRPPADLTITPGTLPRQIPAIPVGVPADLLQRRPDVAAAERAME
QYNAQIGADMAAFFPDVTLTADYSYSGDPIGQLVQVVNRIWSLGASASEVLFQGGSRMAAVHGANAQYDSAVATYRQTVL
TALQNTEDNLSNLRILEQQATQQQIALDFANQAVQVSLNQYEAGTQIYTTVITNETTALSNAESALSIQQQRMVDSVALV
QALGGGWNATSLPSKASMQTDNPFLPSFIQKDKNQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=478, Percent_Identity=27.1966527196653, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI2367348, Length=442, Percent_Identity=24.8868778280543, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55950; Mature: 55819

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPFSLMSRRGALHLGGILAPLALAGCMVGPSYHRPAAPVSARFKELTPAAGWERARPA
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCHHCCCH
MAELPKGAWWTIYNDPVLNRLESQVAISNQNVRMYEANYRQARAMIDSVRAQLFPTLSGS
HHHCCCCCEEEEECCHHHHHHHHHHEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCC
LGFNRNSQGRGSRSASTGSLVSYGGAATNTTETTYSMGPSASWDLDLWGRIRRNIQSQVT
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
EAQASAADLANATLSYQAQLATAYFNLRYQDSLQELLRRYVDFNTQALQITQNQYEAGTA
HHHHHHHHHHHHHHHHHHHEEEHHEECEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
DPTAVMQARTQLEQNRATLIQAGIARAQYEHAIAVLMGRPPADLTITPGTLPRQIPAIPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEECCCCCCCCCCCCCC
GVPADLLQRRPDVAAAERAMEQYNAQIGADMAAFFPDVTLTADYSYSGDPIGQLVQVVNR
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHHH
IWSLGASASEVLFQGGSRMAAVHGANAQYDSAVATYRQTVLTALQNTEDNLSNLRILEQQ
HHHCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ATQQQIALDFANQAVQVSLNQYEAGTQIYTTVITNETTALSNAESALSIQQQRMVDSVAL
HHHHHHHHHHHCCEEEEEHHHHCCCCEEEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHH
VQALGGGWNATSLPSKASMQTDNPFLPSFIQKDKNQ
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TSPFSLMSRRGALHLGGILAPLALAGCMVGPSYHRPAAPVSARFKELTPAAGWERARPA
CCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCHHCCCH
MAELPKGAWWTIYNDPVLNRLESQVAISNQNVRMYEANYRQARAMIDSVRAQLFPTLSGS
HHHCCCCCEEEEECCHHHHHHHHHHEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCC
LGFNRNSQGRGSRSASTGSLVSYGGAATNTTETTYSMGPSASWDLDLWGRIRRNIQSQVT
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
EAQASAADLANATLSYQAQLATAYFNLRYQDSLQELLRRYVDFNTQALQITQNQYEAGTA
HHHHHHHHHHHHHHHHHHHEEEHHEECEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
DPTAVMQARTQLEQNRATLIQAGIARAQYEHAIAVLMGRPPADLTITPGTLPRQIPAIPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEECCCCCCCCCCCCCC
GVPADLLQRRPDVAAAERAMEQYNAQIGADMAAFFPDVTLTADYSYSGDPIGQLVQVVNR
CCCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHHH
IWSLGASASEVLFQGGSRMAAVHGANAQYDSAVATYRQTVLTALQNTEDNLSNLRILEQQ
HHHCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ATQQQIALDFANQAVQVSLNQYEAGTQIYTTVITNETTALSNAESALSIQQQRMVDSVAL
HHHHHHHHHHHCCEEEEEHHHHCCCCEEEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHH
VQALGGGWNATSLPSKASMQTDNPFLPSFIQKDKNQ
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]