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The map label for this gene is cobS [H]

Identifier: 162146666

GI number: 162146666

Start: 861736

End: 862488

Strand: Reverse

Name: cobS [H]

Synonym: GDI_0844

Alternate gene names: 162146666

Gene position: 862488-861736 (Counterclockwise)

Preceding gene: 162146667

Following gene: 162146661

Centisome position: 21.87

GC content: 75.43

Gene sequence:

>753_bases
ATGACCTGGCTTACCCGAGTCCGCGCGGACCTCGCCACGGGCCTGGGCCTGCTGACCCGGCTGCCGGTGTCCTGGCTGGC
GCGGGGCAGCACGGGCGTCGACCTGGCGCGGTCGGCCTGGACATGGCCCCTGGCGGGGCTGGCGATCGGGGCCTTGGGGG
GCACCGTCCTGACCGTGCTGGCTGAGGCCGGCATCGGCCGCCTGCCGGCGGCGGGATGGGCCGTCGCGGCGCAGATCCTG
CTGACCGGGGGACTGCACGAGGACGGGCTGGCCGACATGGCCGACGGGTTCGGCGGCGGCCGGGACGCCGCGCGCAAGCT
GGCGATCATGCGCGACAGCCGCGTGGGCAGTTACGGGGTCATGGCGCTGGGCCTGGCGCTGCTGATCCGGGCGACGGCGG
CGGCGGACCTGCCGCACCCCGTCGCGGCCATGGCGGTGGCCGGGGGCCTGGGCCGGGCCGCGATGGCCGGCCTGGTGGCG
GTGCTGCCCCCGGCCCGCCAGGATGGAATGGCCGCCACGATGACGCGCATTCCGCCGCCCGTTCTGGTCGCCAGCCTGCT
GACGCCCGTGATCGCCGCGCGGCTGACGCTGACGCAGTTCCAGACGGGCAGCGTCTGCCTGGCCGCCTGCGCGGCGGCAA
TATTGATGGGCCGGATGGCCCGGCGCCAGGTCGGCGGACAGACCGGCGACATCCTGGGCGCCACCGCCCTGATCATCGAA
TGCAGCGTACTGAGCGTACTGACGGCCGCCTGA

Upstream 100 bases:

>100_bases
CGCTGGCCGTGCCGCTGCTGCGCGCGGCGGCAGCCTGTCATGGCGGCATGGCGACATTCGCCGAGGCCGAAGTCAGCGAG
AAGAAAGTCAGCGGGGAAAC

Downstream 100 bases:

>100_bases
CGCCATCACCCGGACACATCACCTTGGCACCATCACATCGTCGCGCTCCGCCGGACGACCGCTTGCGCCCGGGCGCCACG
CCCCCACATGCGGCCTGTCG

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTWLTRVRADLATGLGLLTRLPVSWLARGSTGVDLARSAWTWPLAGLAIGALGGTVLTVLAEAGIGRLPAAGWAVAAQIL
LTGGLHEDGLADMADGFGGGRDAARKLAIMRDSRVGSYGVMALGLALLIRATAAADLPHPVAAMAVAGGLGRAAMAGLVA
VLPPARQDGMAATMTRIPPPVLVASLLTPVIAARLTLTQFQTGSVCLAACAAAILMGRMARRQVGGQTGDILGATALIIE
CSVLSVLTAA

Sequences:

>Translated_250_residues
MTWLTRVRADLATGLGLLTRLPVSWLARGSTGVDLARSAWTWPLAGLAIGALGGTVLTVLAEAGIGRLPAAGWAVAAQIL
LTGGLHEDGLADMADGFGGGRDAARKLAIMRDSRVGSYGVMALGLALLIRATAAADLPHPVAAMAVAGGLGRAAMAGLVA
VLPPARQDGMAATMTRIPPPVLVASLLTPVIAARLTLTQFQTGSVCLAACAAAILMGRMARRQVGGQTGDILGATALIIE
CSVLSVLTAA
>Mature_249_residues
TWLTRVRADLATGLGLLTRLPVSWLARGSTGVDLARSAWTWPLAGLAIGALGGTVLTVLAEAGIGRLPAAGWAVAAQILL
TGGLHEDGLADMADGFGGGRDAARKLAIMRDSRVGSYGVMALGLALLIRATAAADLPHPVAAMAVAGGLGRAAMAGLVAV
LPPARQDGMAATMTRIPPPVLVASLLTPVIAARLTLTQFQTGSVCLAACAAAILMGRMARRQVGGQTGDILGATALIIEC
SVLSVLTAA

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes Ado-cobalamin 5'-phosphate from Ado-cobinamide-GDP and alpha-ribazole 5'- phosphate [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=228, Percent_Identity=35.0877192982456, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 25130; Mature: 24999

Theoretical pI: Translated: 10.98; Mature: 10.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWLTRVRADLATGLGLLTRLPVSWLARGSTGVDLARSAWTWPLAGLAIGALGGTVLTVL
CCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AEAGIGRLPAAGWAVAAQILLTGGLHEDGLADMADGFGGGRDAARKLAIMRDSRVGSYGV
HHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHH
MALGLALLIRATAAADLPHPVAAMAVAGGLGRAAMAGLVAVLPPARQDGMAATMTRIPPP
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHH
VLVASLLTPVIAARLTLTQFQTGSVCLAACAAAILMGRMARRQVGGQTGDILGATALIIE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
CSVLSVLTAA
HHHHHHHHCC
>Mature Secondary Structure 
TWLTRVRADLATGLGLLTRLPVSWLARGSTGVDLARSAWTWPLAGLAIGALGGTVLTVL
CHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AEAGIGRLPAAGWAVAAQILLTGGLHEDGLADMADGFGGGRDAARKLAIMRDSRVGSYGV
HHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHH
MALGLALLIRATAAADLPHPVAAMAVAGGLGRAAMAGLVAVLPPARQDGMAATMTRIPPP
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHH
VLVASLLTPVIAARLTLTQFQTGSVCLAACAAAILMGRMARRQVGGQTGDILGATALIIE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
CSVLSVLTAA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1917841 [H]