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The map label for this gene is tsf [H]

Identifier: 161870971

GI number: 161870971

Start: 2102580

End: 2103434

Strand: Direct

Name: tsf [H]

Synonym: NMCC_2066

Alternate gene names: 161870971

Gene position: 2102580-2103434 (Clockwise)

Preceding gene: 161870970

Following gene: 161870972

Centisome position: 97.64

GC content: 52.75

Gene sequence:

>855_bases
ATGGCAGAAATTACTGCAAAAATGGTTGCCGATCTGCGCGCCGCTACCGGCCTGGGCATGATGGAATGCAAAAAAGCCTT
GGTTGAAGCCGAAGGCAACTTCGACAAAGCCGAAGAAATCCTGCGTATCAAATCCGGTGCGAAAGCCGGTAAACTGGCCG
GCCGTACCGCTGCCGAAGGCGTATTGGCTTACGCGATCAACGGCAATGTCGGCGCATTGGTCGAAGTAAACTGCGAAACC
GACTTCGTTGCGAAAGACGCGGGCTTCGTAGAATTTGCCAACTTCGTTGCGAAAACCGCCGCCGAGAAAAAACCGGCTTC
TGTTGAAGAACTGAGCGAACTGGTTGAAGCAGAACGCAAAGCCATCATCGCCAAATTGGGCGAGAACATGTCTGTCCGTC
GCTTCCAAGTGATCGACACTGCCAACCAACTGGTTGCCTACATCCACGGCGCATTGGCGACCGAAGGCGTATTGGTTGAG
TACAAAGGCTCTGAAGACGTAGCACGCAAAATCGGTATGCACATTGTTGCCGCTAAACCACAATGCGTAAGCGAAGCCGA
AGTAGATGCCGAAACCGTTGAAAAAGAACGCCACATCTACACCGAACAAGCCATCGCTTCCGGCAAACCTGCCGACATCG
CCGCTAAAATGGTTGAAGGCCGTATCCGCAAATTCTTGGCTGAAATCACTCTGAACGGCCAAGCATTCGTGATGAACCCC
GATCAAACTGTTGCCCAATTCGCCAAAGAAAACGGCACTGAAGTGGTCAGCTTCGTCCGCTACAAAGTAGGCGACGGTAT
CGAGAAAGCCGTTGTCGACTACGCAGCCGAAGTAGCCGCAGCCGCCAAAGTGTAA

Upstream 100 bases:

>100_bases
CCCTTTTCTCAAATATGCCGTCTGAACGTCCGTTCGCGGCACACGATTCCCGAATGCGGAAAATCCTTTCCGTATTTCCC
AAAAATCTAGGAGATTCAAA

Downstream 100 bases:

>100_bases
GGCACTTATGAAAAAGAAAGCACCTGGATTCCAAACGAATCAGGGTGCTTTTTTTTGAGAAAACCGTTTGCGGTACCTAT
TTTAAGACGACCGAATATTC

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIKSGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCET
DFVAKDAGFVEFANFVAKTAAEKKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMVEGRIRKFLAEITLNGQAFVMNP
DQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAAEVAAAAKV

Sequences:

>Translated_284_residues
MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIKSGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCET
DFVAKDAGFVEFANFVAKTAAEKKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMVEGRIRKFLAEITLNGQAFVMNP
DQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAAEVAAAAKV
>Mature_283_residues
AEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIKSGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETD
FVAKDAGFVEFANFVAKTAAEKKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVEY
KGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMVEGRIRKFLAEITLNGQAFVMNPD
QTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAAEVAAAAKV

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=301, Percent_Identity=27.5747508305648, Blast_Score=95, Evalue=8e-20,
Organism=Escherichia coli, GI1786366, Length=285, Percent_Identity=51.2280701754386, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17561440, Length=292, Percent_Identity=27.3972602739726, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI19921466, Length=305, Percent_Identity=26.8852459016393, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 30271; Mature: 30140

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIKSGAKAGKLAGRTAAEG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCHHHCC
VLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAEKKPASVEELSELVEAERK
EEEEEEECCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVEYKGSEDVARKIGMHIVAAKP
HHHHHHCCCCCEEHEEHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCEEEECCC
QCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMVEGRIRKFLAEITLNGQAFVMNP
CHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCEEEEECC
DQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAAEVAAAAKV
HHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIKSGAKAGKLAGRTAAEG
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCHHHCC
VLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAEKKPASVEELSELVEAERK
EEEEEEECCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVEYKGSEDVARKIGMHIVAAKP
HHHHHHCCCCCEEHEEHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCEEEECCC
QCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMVEGRIRKFLAEITLNGQAFVMNP
CHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCEEEEECC
DQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAAEVAAAAKV
HHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA