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The map label for this gene is topA [H]

Identifier: 161870936

GI number: 161870936

Start: 2072374

End: 2074620

Strand: Reverse

Name: topA [H]

Synonym: NMCC_2030

Alternate gene names: 161870936

Gene position: 2074620-2072374 (Counterclockwise)

Preceding gene: 161870937

Following gene: 161870933

Centisome position: 96.34

GC content: 54.07

Gene sequence:

>2247_bases
TTGGGCGGCGATTTTGAAATCCTTGCATCCTACGGACACGTCCGCGACCTCGTCCCCAAAAGCGGCGCGGTCGATCCCGA
CAACGGCTTTGCGATGAAATACCAACTCATCAGCCGCAACGGCAAACACGTCGATGCCATCGTCGCCGGTGCCAAAGAAG
CTGAAAACATCTACCTCGCCACCGACCCGGATAGGGAAGGCGAAGCCATTTCCTGGCATCTTTTGGAAATCCTCAAATCC
AAACGCGGCTTGAAAAACATCAAGCCGCAGCGTGTCGTGTTCCACGAAATCACCAAAAACGCCGTGCTCGATGCCGTTGC
CCATCCGCGCGAAATCGAAATGGACTTGGTCGATGCGCAACAAGCCCGCCGTGCTTTGGACTATTTGGTCGGCTTTAACC
TTTCGCCATTGTTATGGAAAAAAATCCGCCGAGGTTTGAGCGCGGGGCGTGTGCAAAGCCCTGCTTTGCGCCTGATTTGC
GAACGCGAAAACGAAATCCGCGCGTTTGAAGCGCAGGAATATTGGACGGTGCATCTCGACAGCCACAAAGGCCGCAGCAA
GTTCACCGCCAAACTCGCCCAATACAACGGCGCGAAACTCGAACAATTCGACCTGCCGAACGAAGCCGCGCAAGCCGACG
TATTGAAAGAACTCGAAGGCAAAGAAGCCGTCGTTACCGCCATTGAAAAGAAAAAGCGCAGCCGCAATCCTGCCGCGCCG
TTCACCACTTCTACCATGCAGCAGGATGCCGTGCGCAAACTCGGCTTCACCACCGACCGCACCATGCGTACCGCCCAACA
GCTTTATGAAGGTATAGACGTAGGGCAGGGCGCGATCGGTCTGATTACCTATATGCGTACCGACAGCGTGAATTTGGCGG
ATGAAGCGTTAACCGAAATCCGCCATTACATTGAAAACAAAATCGGCAAAGAATATCTGCCGAGTGCCGCCAAACAATAC
AAAACCAAATCCAAAAACGCCCAAGAAGCCCACGAAGCCATCCGTCCGACTTCCGTGTACCGCACGCCCGAAAGCGTCAA
ACCCTTCCTGAGCGCAGACCAGTTCAAACTCTATCAAATGATTTGGCAGCGCACCGTCGCCTGTCAGATGACGCCTGCCA
AATTCGACCAAACCACCGTCGATATTACCGTCGGCAAAGGCGTATTCCGCGTAACCGGACAAGTGCAAACCTTCGCAGGC
TTTTTGAGCGTGTATGAAGAAAGCAGCGATGACGAAGAAGGCGAAGACAGCAAAAAACTGCCCGAAATGAGCGAAGGCGA
TAAATTGCCCGTGGACAAACTCTACGGCGAACAACACTTTACCACTCCGCCGCCACGCTACAACGAAGCCACGCTGGTTA
AAGCCCTCGAAGAATACGGCATCGGCCGCCCCTCGACCTACGCCAGCATCATCTCCACGCTCAAAGACCGCGAATACGTT
ACCCTTGAGCAAAAACGCTTCATGCCCACCGACACAGGCGACATCGTCAACAAATTCCTGACCGAACACTTCGCCCAATA
CGTCGATTACCACTTTACCGCCAAACTCGAAGACCAGCTTGACGAAATCGCCGGCGGCAAACGCCAATGGATTCCCTTGA
TGGACAAATTCTGGAAACCGTTCATCAAACAAGTGGAAGAAAAAGAAGGCATCGAACGCGCCAAATTTACCACGCAGGAA
CTTGATGAAACCTGCCCGAAATGCGGCGAACACAAACTGCAAATCAAGTTCGGCAAAATGGGCCGTTTCGTTGCGTGTGC
CGGCTATCCCGAGTGCAGCTACACGCGCAATGTCAATGAAACCGCCGAAGAAGCCGCAGAGCGCATCGCCAAAGCCGAAG
CCGAACAGGCAGAACTCGACGGACGCGAATGTCCTAAATGTGGTGGTCGCCTAGTGTACAAATACAGCCGAACCGGCAGC
AAATTCATCGGCTGCGCCAACTATCCGAAATGCAAACACGTCGAGCCGCTGGAAAAACCGAAAGATACCGGCGTCCAGTG
TCCGCAATGCAAAAAAGGCAACCTCGTCGAGCGCAAATCCCGCTACGGCAAACTGTTTTACAGCTGCAGCACCTATCCCG
ACTGCAACTACGCCACCTGGAACCCGCCCGTCGCCGAAGAATGCCCGAACTGCCATTGGCCGGTCTTGACCATCAAAACC
ACCAAACGCTGGGGCGTCGAAAAAGTCTGCCCGCAAAAAGAATGCGGCTGGAAAGAACAGATTGAACCGCCCGCGCCGAA
GGAGTAA

Upstream 100 bases:

>100_bases
TGAAACGCCGGCATCAAAACCACCATCCAGAGAACGACAAATGGCGAAAAACCTATTAATCGTCGAATCCCCGTCCAAAG
CCAAAACCCTGAAAAAATAT

Downstream 100 bases:

>100_bases
GATTAGGTTGGTTTGAAAGAGAAAAGGTCGTCTGAAAAATTTTCAGACGACCTTTGCTTTTCTGTGATTGGTTTATTTGA
ATCCGCGTGTTGTTTTAAAG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 748; Mature: 747

Protein sequence:

>748_residues
MGGDFEILASYGHVRDLVPKSGAVDPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKS
KRGLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWKKIRRGLSAGRVQSPALRLIC
ERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGAKLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAP
FTTSTMQQDAVRKLGFTTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLPSAAKQY
KTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTPAKFDQTTVDITVGKGVFRVTGQVQTFAG
FLSVYEESSDDEEGEDSKKLPEMSEGDKLPVDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYV
TLEQKRFMPTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKPFIKQVEEKEGIERAKFTTQE
LDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEEAAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGS
KFIGCANYPKCKHVEPLEKPKDTGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
TKRWGVEKVCPQKECGWKEQIEPPAPKE

Sequences:

>Translated_748_residues
MGGDFEILASYGHVRDLVPKSGAVDPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKS
KRGLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWKKIRRGLSAGRVQSPALRLIC
ERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGAKLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAP
FTTSTMQQDAVRKLGFTTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLPSAAKQY
KTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTPAKFDQTTVDITVGKGVFRVTGQVQTFAG
FLSVYEESSDDEEGEDSKKLPEMSEGDKLPVDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYV
TLEQKRFMPTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKPFIKQVEEKEGIERAKFTTQE
LDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEEAAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGS
KFIGCANYPKCKHVEPLEKPKDTGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
TKRWGVEKVCPQKECGWKEQIEPPAPKE
>Mature_747_residues
GGDFEILASYGHVRDLVPKSGAVDPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSK
RGLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWKKIRRGLSAGRVQSPALRLICE
RENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGAKLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPF
TTSTMQQDAVRKLGFTTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLPSAAKQYK
TKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTPAKFDQTTVDITVGKGVFRVTGQVQTFAGF
LSVYEESSDDEEGEDSKKLPEMSEGDKLPVDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVT
LEQKRFMPTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKPFIKQVEEKEGIERAKFTTQEL
DETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEEAAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSK
FIGCANYPKCKHVEPLEKPKDTGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKTT
KRWGVEKVCPQKECGWKEQIEPPAPKE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=627, Percent_Identity=24.7208931419458, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI4507635, Length=663, Percent_Identity=24.2835595776772, Blast_Score=132, Evalue=2e-30,
Organism=Escherichia coli, GI1787529, Length=746, Percent_Identity=39.6782841823056, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI48994931, Length=183, Percent_Identity=44.8087431693989, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788061, Length=532, Percent_Identity=27.4436090225564, Blast_Score=130, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32563869, Length=558, Percent_Identity=25.089605734767, Blast_Score=122, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=507, Percent_Identity=25.0493096646943, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=498, Percent_Identity=22.6907630522088, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585251, Length=637, Percent_Identity=23.861852433281, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24640096, Length=555, Percent_Identity=22.8828828828829, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 84738; Mature: 84607

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGDFEILASYGHVRDLVPKSGAVDPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEHHHHHCCCCCCEEEEE
TDPDREGEAISWHLLEILKSKRGLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQ
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QARRALDYLVGFNLSPLLWKKIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLD
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEECCCEEEEEEE
SHKGRSKFTAKLAQYNGAKLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAP
CCCCHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
FTTSTMQQDAVRKLGFTTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEI
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHH
RHYIENKIGKEYLPSAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQM
HHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHCCCCHHHHHHHCHHHHHHHHH
IWQRTVACQMTPAKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKL
HHHHHHHEEECCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHCC
PEMSEGDKLPVDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYV
CCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
TLEQKRFMPTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKP
EEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCCCCHHHHHHHHH
FIKQVEEKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNE
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCCCHH
TAEEAAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCC
KDTGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
CCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCEEEEEE
TKRWGVEKVCPQKECGWKEQIEPPAPKE
CCCCCHHHCCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure 
GGDFEILASYGHVRDLVPKSGAVDPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEHHHHHCCCCCCEEEEE
TDPDREGEAISWHLLEILKSKRGLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQ
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QARRALDYLVGFNLSPLLWKKIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLD
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEECCCEEEEEEE
SHKGRSKFTAKLAQYNGAKLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAP
CCCCHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
FTTSTMQQDAVRKLGFTTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEI
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHH
RHYIENKIGKEYLPSAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQM
HHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHCCCCHHHHHHHCHHHHHHHHH
IWQRTVACQMTPAKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKL
HHHHHHHEEECCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHCC
PEMSEGDKLPVDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYV
CCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
TLEQKRFMPTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKP
EEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCCCCHHHHHHHHH
FIKQVEEKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNE
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCCCHH
TAEEAAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCC
KDTGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
CCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCEEEEEE
TKRWGVEKVCPQKECGWKEQIEPPAPKE
CCCCCHHHCCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]