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The map label for this gene is kefBC [H]

Identifier: 161870853

GI number: 161870853

Start: 1991481

End: 1993628

Strand: Reverse

Name: kefBC [H]

Synonym: NMCC_1940

Alternate gene names: 161870853

Gene position: 1993628-1991481 (Counterclockwise)

Preceding gene: 161870855

Following gene: 161870849

Centisome position: 92.58

GC content: 55.59

Gene sequence:

>2148_bases
ATGGCGGCGGCTGTGGCGGTCATGTCGGTGTGCGGCTCAAAATCGAAAGGGCGTATTTTAGCAGAATTGTATGCCGCGCC
CGTTTCGGATGGTGCGCGGTGTTTTGTTATAATACGGCGGCGTATGCCGTTTCAGACGGCATTTTTCTGTATTTTCCTGT
TCGGACGGCCTATGAACGAATTTTCGCTTGCCCCTATTGTGGTTGTTTTGCTGGTGTCGGTCATTACGGTGATCCTGTGC
CGCAAGTTCAACATTCCCTCCATGCTGGGCTACCTGCTGGTGGGCTTTTTGGCGGGGCCCGGTATGCTCAGCCTGATTCC
GAAAAGCCATGCGACGGATTATTTGGGCGAAATCGGGATTGTGTTCCTGATGTTCAGTATAGGCTTGGAGTTTTCGCTGC
CCAAGCTGAGGGCAATGAGGCGGCTGGTGTTCGGGCTGGGTGGTTTGCAGGTCGGCGTTACGATGCTGTCGGTAATGGGC
ATACTGATGCTGACGGGCGTCCCGTTCAATTGGGCGTTTGCCGTGTCGGGCGCGTTGGCGATGTCGTCCACGGCGATTGT
GAGCCGGATTTTGTCGGAAAAGACGGAATTGGGGCAGCCGCACGGTCAGATGGCGATGGGCGTGCTGCTGATGCAGGACA
TCGCCGTCGTGCCGCTGATGATTCTGATTCCCGCGCTGGCGGGCGGAGGGGACGGAAATATTTGGGCGGCCTTGGGTTTG
GCGTTTGCAAAAATGCTGCTGACGCTGGGGCTGCTGTTTTTCGTCGGCAGCAAAATTATGTCGCGATGGTTCAGGATGGT
GGCAAAACGCAAATCGTCCGAACTCTTTATGATCAATGTGCTGCTGGTAACCTTGGGTGTGGCTTATCTGACTGAGCTGG
AAGGTTTGTCTATGGCGTTGGGCGCATTCGTTGCCGGCATGCTGCTTTCGGAAACGGAATACCGTTTCCAAGTCGAAGAC
GACATCCGCCCGTTCCGCGATATTTTGCTCGGCTTTTTCTTTATCACGGTCGGCATGAAGCTGGACATTCAGGCATTGAT
CGGCGGCTGGCGGCAGGTATTGATGCTGTTGGCAATGCTGCTGGTGTTGAAGGCACTGGTTGTGTTTGCCATTGCCTTCA
AAATGAAACATTCGGTCGGCGACAGCCTCAAAACGGCTTTGTATCTCGCGCAGGGCGGCGAGTTCGGCTTCGTGATGCTG
GCCATTGCCGGGCAGCTTGATATGGTTTCGCCAGAATGGGAACAGGCGGCGACGGCGGCGGTTCTGCTGTCGATGATTAT
CGCGCCCTTCCTCTTGGGCGGCAGCGATGCGCTGGTCGGGCGTTTGGTCAAGTCAAGCTGGGACATGAAGTCGCTCGATC
TGCACAGTATGCTGGTAGAAACCATGAGCAAGTCCGACCATGTGCTGATTGTCGGCTTCGGCAGGGGCGGGCAGACGGTC
GGACGCGTCCTTGCCCAAGAGGATATTCCGTATTTCGCGCTCGACTTGGACATTGCGCGGGTGCAGGTTGCCAGAAGTGC
GGGCGAACCGGTGTCGTTCGGCGATGCGAAACGCAGGGAAGTATTGGAAGCCGCCGGTCTGGGACGGGCGAAAATGGTGG
TGGTTACGCTCAACAATATGCACGAAACGCAACACGTTTTAGACAATGTGCTGTCCATGTATCCCAATATGCCCGTATAT
GTGCGCGCCACCAACGACGATTATGTGAAAACGTTTACCGATATAGGTGCGGAAGAAGCCGTGTCGGACACCAAAGAAAC
CGGACTCGTGCTGGCAGGCTATGCGATGTTAGGCAACGGCGCGTCGTATCGGCACGTCTATCAGACGATGGCAAATATCC
GCCACAGCCGTTATGCCGCGTTGGAGGGACTGTTTGTCGGTAGTGATGATGAGGCAGGATTCGGCGAAACCGGCGAAACC
GTCCGCCACGCCTTTCCTTTGGCTGCAGAAGCATACGCCGTCGGCAAAACAGTCGGCACGCTTCCGATGGCGGCTTACGG
CATCAAACTCTTGTTCGTCCGCCGCCGCACCGGCCGGATTGAAAACCCGGATGCCTCGTTTACATTGGAAGGCGGTGACG
TGTTGGTGGTCGCAGGCAAAAAAGAAGAAATTATCTCTTTTGAAAACTGGAGTTTGCAGGGAATATAA

Upstream 100 bases:

>100_bases
GCAGAGGTTGTACGCTTCGCCTGTTGCGACTGCCTGTGCGTAGGGCAGGCAGCCGTCGTAGCCGCATTCGCGGCATTGGG
TTTGGGGAAGCAGGCGGTCT

Downstream 100 bases:

>100_bases
ATGAAATGCCGAAATAAGGCTTGCGCCATTTCCGGTTATTTGGTTTAATAACGCTTTCGCAAATCGCAAGGGTGATTAGC
TCAGTTGGTAGAGCGTCTGC

Product: glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MAAAVAVMSVCGSKSKGRILAELYAAPVSDGARCFVIIRRRMPFQTAFFCIFLFGRPMNEFSLAPIVVVLLVSVITVILC
RKFNIPSMLGYLLVGFLAGPGMLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVMG
ILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVVPLMILIPALAGGGDGNIWAALGL
AFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSELFMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVED
DIRPFRDILLGFFFITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQGGEFGFVML
AIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDMKSLDLHSMLVETMSKSDHVLIVGFGRGGQTV
GRVLAQEDIPYFALDLDIARVQVARSAGEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVY
VRATNDDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEGLFVGSDDEAGFGETGET
VRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIENPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI

Sequences:

>Translated_715_residues
MAAAVAVMSVCGSKSKGRILAELYAAPVSDGARCFVIIRRRMPFQTAFFCIFLFGRPMNEFSLAPIVVVLLVSVITVILC
RKFNIPSMLGYLLVGFLAGPGMLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVMG
ILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVVPLMILIPALAGGGDGNIWAALGL
AFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSELFMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVED
DIRPFRDILLGFFFITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQGGEFGFVML
AIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDMKSLDLHSMLVETMSKSDHVLIVGFGRGGQTV
GRVLAQEDIPYFALDLDIARVQVARSAGEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVY
VRATNDDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEGLFVGSDDEAGFGETGET
VRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIENPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI
>Mature_714_residues
AAAVAVMSVCGSKSKGRILAELYAAPVSDGARCFVIIRRRMPFQTAFFCIFLFGRPMNEFSLAPIVVVLLVSVITVILCR
KFNIPSMLGYLLVGFLAGPGMLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVMGI
LMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVVPLMILIPALAGGGDGNIWAALGLA
FAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSELFMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDD
IRPFRDILLGFFFITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQGGEFGFVMLA
IAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDMKSLDLHSMLVETMSKSDHVLIVGFGRGGQTVG
RVLAQEDIPYFALDLDIARVQVARSAGEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYV
RATNDDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEGLFVGSDDEAGFGETGETV
RHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIENPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=376, Percent_Identity=26.063829787234, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786232, Length=534, Percent_Identity=32.7715355805243, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789749, Length=555, Percent_Identity=33.5135135135135, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1786685, Length=530, Percent_Identity=29.811320754717, Blast_Score=212, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 77369; Mature: 77238

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAVAVMSVCGSKSKGRILAELYAAPVSDGARCFVIIRRRMPFQTAFFCIFLFGRPMNE
CHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCC
FSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPGMLSLIPKSHATDYLGEIGI
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHH
VFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVMGILMLTGVPFNWAFAVSGALA
HHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
MSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVVPLMILIPALAGGGDGNIWAALGL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSELFMINVLLVTLGVAYLTELEGLSMAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GAFVAGMLLSETEYRFQVEDDIRPFRDILLGFFFITVGMKLDIQALIGGWRQVLMLLAML
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LVLKALVVFAIAFKMKHSVGDSLKTALYLAQGGEFGFVMLAIAGQLDMVSPEWEQAATAA
HHHHHHHHHHHHHHHHHHHCHHHHHHHHEECCCCCCEEEEEECCCCCCCCCCHHHHHHHH
VLLSMIIAPFLLGGSDALVGRLVKSSWDMKSLDLHSMLVETMSKSDHVLIVGFGRGGQTV
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHH
GRVLAQEDIPYFALDLDIARVQVARSAGEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNM
HHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCH
HETQHVLDNVLSMYPNMPVYVRATNDDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNG
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCHHHHHCCHHHCCEEEEEHHHHCCC
ASYRHVYQTMANIRHSRYAALEGLFVGSDDEAGFGETGETVRHAFPLAAEAYAVGKTVGT
CHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHC
LPMAAYGIKLLFVRRRTGRIENPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI
CHHHHHHHHHEEEEHHCCCCCCCCCCEEECCCCEEEEECCCHHEEEECCCCCCCC
>Mature Secondary Structure 
AAAVAVMSVCGSKSKGRILAELYAAPVSDGARCFVIIRRRMPFQTAFFCIFLFGRPMNE
HHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCC
FSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPGMLSLIPKSHATDYLGEIGI
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHH
VFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVMGILMLTGVPFNWAFAVSGALA
HHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
MSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVVPLMILIPALAGGGDGNIWAALGL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSELFMINVLLVTLGVAYLTELEGLSMAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GAFVAGMLLSETEYRFQVEDDIRPFRDILLGFFFITVGMKLDIQALIGGWRQVLMLLAML
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LVLKALVVFAIAFKMKHSVGDSLKTALYLAQGGEFGFVMLAIAGQLDMVSPEWEQAATAA
HHHHHHHHHHHHHHHHHHHCHHHHHHHHEECCCCCCEEEEEECCCCCCCCCCHHHHHHHH
VLLSMIIAPFLLGGSDALVGRLVKSSWDMKSLDLHSMLVETMSKSDHVLIVGFGRGGQTV
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHH
GRVLAQEDIPYFALDLDIARVQVARSAGEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNM
HHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCH
HETQHVLDNVLSMYPNMPVYVRATNDDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNG
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCHHHHHCCHHHCCEEEEEHHHHCCC
ASYRHVYQTMANIRHSRYAALEGLFVGSDDEAGFGETGETVRHAFPLAAEAYAVGKTVGT
CHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHC
LPMAAYGIKLLFVRRRTGRIENPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI
CHHHHHHHHHEEEEHHCCCCCCCCCCEEECCCCEEEEECCCHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]