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The map label for this gene is bioH [H]

Identifier: 161870793

GI number: 161870793

Start: 1926561

End: 1927469

Strand: Reverse

Name: bioH [H]

Synonym: NMCC_1875

Alternate gene names: 161870793

Gene position: 1927469-1926561 (Counterclockwise)

Preceding gene: 161870804

Following gene: 161870792

Centisome position: 89.51

GC content: 60.73

Gene sequence:

>909_bases
ATGAAACACAGGGGGCAGCTGTTTGCCGCGTCGGTGCGGTATGCCGCCAAATCGTCGCGGCAGCCGGCGCAGATGCCGTC
TGAAACGCCGGACGAACCGTGGCATAATACGCAACGCCTGATAGTGGGCGCGTCTGCGATGCGCCGCCAACAAGAGAGAA
AATCCATGCCTGATGCCGTCAAAAAAGTTTACCTGATACACGGTTGGGGGGCGAACCGCCACGTGTTTGACGATTTGATG
CCGCGCCTGCCTGCAACGTGGGCGGTGTCCGCCGTCGATTTGCCCGGACACGGCGACGCGCCCTTCGTGCAACCCTTCGA
TATTGAGGCAGCCGCCGACGCTGTTGCCGCGCAAATCGATACGCCCGCCGACATTCTCGGCTGGTCGCTCGGCGGATTGG
TCGCGCTGTATCTGGCGGCGCGCCATCCCGACAAAGTCCGTTCGCTCTGCCTGACGGCGAGTTTCGCACGGCTGACGGCT
GCCGAAGATTATCCCGAAGGGCTTGCCGCGCCTGCACTGGGCAAAATGGTCGGCGCGTTCCGAACGGATTATGCCAAACA
TATCAAACAGTTTCTACAATTGCAGCTTCTGCACACGCCTGATGCGGACGGAATCATAGGCAGGATTCTGCCCGATTTGG
CGCGCTGCGGCACGCCCTCCGCCCTGCAGGAGGCGTTGGACGCGGCGGAAAGGGCGGATGCGCGGCATTTGTTGGACAAG
ATAGATGTTCCGGTACTGCTGGTGTTCGGCGGCAAAGACGCGATTACACCGCCGCGTATGGGTGAGTATCTGCATCGGCA
TTTGAAAGGCAGCAGGCTGGTTGTGATGGAAAAGGCGGCACACGCGCCGTTTTTGAGCCATGCGGAAGCGTTTGCCGCGC
TGTACCGCGACTTTGTCGAAGGGGTTTGA

Upstream 100 bases:

>100_bases
ATTGCTGACGGGCGGTTCGTAATGCAGCGAAGCCCACATCCGGTCGAATGCGGGCGGTTTTTTCTGACAGCCGCCGCACA
CCGCGCCGCCTTGAACGTGC

Downstream 100 bases:

>100_bases
CATGAATCCGCAGGACAAATGTTGGCAGGTTCACCGCCATCTTGCCGAACATGCCGACCAACGGCTGACACTCGTCCGCA
ACGCGCCCAAACATATCCTG

Product: hydrolase

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLM
PRLPATWAVSAVDLPGHGDAPFVQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTA
AEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGTPSALQEALDAAERADARHLLDK
IDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSHAEAFAALYRDFVEGV

Sequences:

>Translated_302_residues
MKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLM
PRLPATWAVSAVDLPGHGDAPFVQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTA
AEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGTPSALQEALDAAERADARHLLDK
IDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSHAEAFAALYRDFVEGV
>Mature_302_residues
MKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLM
PRLPATWAVSAVDLPGHGDAPFVQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTA
AEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGTPSALQEALDAAERADARHLLDK
IDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSHAEAFAALYRDFVEGV

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=240, Percent_Identity=29.5833333333333, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 32954; Mature: 32954

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKSMPDAV
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEECHHHHHHHHHHHCCHHHH
KKVYLIHGWGANRHVFDDLMPRLPATWAVSAVDLPGHGDAPFVQPFDIEAAADAVAAQID
HHEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCC
TPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTAAEDYPEGLAAPALGKMVGAF
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
RTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGTPSALQEALDAAERADARHLLDK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSHAEAFAALYRDFVE
CCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHH
GV
CC
>Mature Secondary Structure
MKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKSMPDAV
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEECHHHHHHHHHHHCCHHHH
KKVYLIHGWGANRHVFDDLMPRLPATWAVSAVDLPGHGDAPFVQPFDIEAAADAVAAQID
HHEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCC
TPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTAAEDYPEGLAAPALGKMVGAF
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
RTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGTPSALQEALDAAERADARHLLDK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSHAEAFAALYRDFVE
CCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHH
GV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]