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The map label for this gene is ostA [H]

Identifier: 161870783

GI number: 161870783

Start: 1913909

End: 1916287

Strand: Reverse

Name: ostA [H]

Synonym: NMCC_1865

Alternate gene names: 161870783

Gene position: 1916287-1913909 (Counterclockwise)

Preceding gene: 161870785

Following gene: 161870782

Centisome position: 88.99

GC content: 59.77

Gene sequence:

>2379_bases
GTGCTGGCATTGGGCCTCTGCTTCGGCACGCATTGCGCCGCCGCCGATGCCGTTGCGGCGGAGGAAACGGACAATCCGAC
CGCCGGAGGAAGCGTTCGGAGCGTGTCCGAACCCATACAGCCTACCAGCCTGAGCCTCGGTTCGACCTGCCTGTTTTGCA
GTAACGAAAGCGGCAGCCCCGAGAGAACCGAAGCCGCCGTCCAAGGCAGCGGCGAAGCATCCATCCCCGAAGACTATACG
CGCATTGTTGCCGACAGGATGGAAGGACAGTCGCAGGTGCAGGTGCGTGCCGAAGGCAACGTCGTCGTCGAACGCAATCG
GACGACCCTCAATGCCGATTGGGCGGATTACGACCAGTCGGGCGACACCGTTACCGCAGGCGACCGGTTCGCCCTCCAAC
AGGACGGTACGCTGATTCGGGGCGAAACCCTGACCTACAATCTCGAGCAGCAGACCGGGGAAGCGCACAACGTCCGTATG
GAAACCGAACACGGCGGACGGCGGCTGCAAAGCGTCAGCCGCACCGCCGAAATGTTGGGCGAAGGGCATTACAAACTGAC
GGAAACCCAATTCAACACCTGTTCCGCCGGCGATGCCGGCTGGTATGTCAAGGCCGCTTCCGTCGAAGCCGATCGGGAAA
AAGGCATAGGCGTTGCCAAACACGCCGCCTTCGTGTTCGGCGGCGTTCCCATTTTCTACACCCCTTGGGCGGACTTCCCG
CTTGACGGCAACCGCAAAAGCGGCCTGCTCGTTCCCTCACTGTCCGCCGGTTCGGACGGCGTTTCCCTTTCCGTTCCCTA
TTATTTCAACCTTGCCCCCAATCTCGATGCCACGTTCGCGCCCGGCGTGATCGGCGAACGCGGCGCGGTCTTTGACGGGC
AGGTACGCTACCTGCGGCCGGATTATGCCGGCCAGTCCGACCTGACCTGGCTGCCGCACGACAAGAAAAGCGGCAGGAAT
AACCGCTATCAGGCGAAATGGCAGCACCGGCACGACATTTCCGACACGCTTCAGGCGGGTGTCGATTTCAACCAAGTCTC
CGACAGCGGCTACTACCGCGACTTTTACGGCAACAAAGAAATCGCCGGCAACGTCAACCTCAACCGCCGTGTATGGCTGG
ATTATGGCGGCAGGGCGGCGGGCGGCAGCCTGAATGCCGGCCTTTCGGTTCTGAAATACCAGACGCTGGCAAACCAAAGC
GGCTACAAAGACAAACCGTATGCCCTGATGCCGCGCCTTTCCGCCGATTGGCGCAAAAACACCGGCAGGGCGCAAATCGG
CGTGTCCGCCCAATTTACCCGCTTCAGCCACGACAGCCGCCAAGACGGCAGCCGCCTCGTCGTCTATCCCGACATCAAAT
GGGATTTCAGCAACAGCTGGGGTTACGTCCGTCCCAAACTCGGACTGCACGCCACCTATTACAGCCTCAACCGCTTCGGC
AGCCAAGAAGCCCGACGCGTCAGCCGCACTCTGCCCATCGTCAACATCGACAGCGGCATGACCTTCGAACGCAATACGCG
GATGTTCGGCGGCGGAGTCCTGCAAACCCTCGAGCCGCGCCTGTTCTACAACTATATTCCTGCCAAATCCCAAAACGACC
TGCCCAATTTCGATTCGTCGGAAAGCAGCTTCGGCTACGGGCAGCTTTTTCGTGAAAACCTCTATTACGGCAACGACAGG
ATTAACACCGCAAACAGCCTTTCCGCCGCCGTGCAAAGCCGTATTTTGGACGGCGCGACGGGGGAAGAGCGTTTCCGCGC
CGGCATCGGGCAGAAATTCTACTTCAAAAACGACGCAGTCATGCTTGACGGCAGTGTCGGCAAAAAACCGCGCAGCCGTT
CCGACTGGGTGGCATTCGCCTCCAGCGGCATCGGCAGCCGCTTCATCCTCGACAGCAGCATCCACTACAACCAAAACGAC
AAACGCGCCGAGAACTACGCCGTCGGTGCAAGCTACCGTCCCGCACAGGGCAAAGTGCTGAACGCCCGCTACAAATACGG
GCGCAACGAAAAAATCTACCTGAAGTCCGACGGTTCCTATTTTTACGACAAACTCAGCCAGCTCGACCTGTCCGCACAAT
GGCCGCTGACGCGCAACCTGTCGGCCGTCGTCCGTTACAACTACGGTTTTGAAGCCAAAAAACCGATAGAGGTGCTGGCG
GGTGCGGAATACAAAAGCAGTTGCGGCTGCTGGGGCGCGGGCGTGTACGCCCAACGCTACGTTACCGGCGAAAACACCTA
CAAAAACGCTGTCTTTTTCTCACTTCAGTTGAAAGACCTCAGCAGTGTCGGCAGAAACCCCGCAGACAGGATGGATGTCG
CCGTTCCCGGCTATATCCCCGCCCACTCTCTTTCCGCCGGACGCAACAAACGGCCCTGA

Upstream 100 bases:

>100_bases
TAACAGTACTAAAGCATTTCAGGTTACAATAAACGCTATTCTAACTGGCAAACCGACTTGAGGGGCGATTTTGGCTCGTT
TATTTTCACTCAAACCACTG

Downstream 100 bases:

>100_bases
CCGTCGGAAACCCGGCAGGAGCACCGTTCCCGCACAAGACGGCATTCCACCGACAACCCCAAACCCGCCATCAAAGGCAG
GATTCAAACGATAAGGAAAG

Product: organic solvent tolerance protein, putative

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 792; Mature: 792

Protein sequence:

>792_residues
MLALGLCFGTHCAAADAVAAEETDNPTAGGSVRSVSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYT
RIVADRMEGQSQVQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQQTGEAHNVRM
ETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASVEADREKGIGVAKHAAFVFGGVPIFYTPWADFP
LDGNRKSGLLVPSLSAGSDGVSLSVPYYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRN
NRYQAKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGGSLNAGLSVLKYQTLANQS
GYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSRQDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFG
SQEARRVSRTLPIVNIDSGMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYGNDR
INTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRSDWVAFASSGIGSRFILDSSIHYNQND
KRAENYAVGASYRPAQGKVLNARYKYGRNEKIYLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLA
GAEYKSSCGCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGRNKRP

Sequences:

>Translated_792_residues
MLALGLCFGTHCAAADAVAAEETDNPTAGGSVRSVSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYT
RIVADRMEGQSQVQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQQTGEAHNVRM
ETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASVEADREKGIGVAKHAAFVFGGVPIFYTPWADFP
LDGNRKSGLLVPSLSAGSDGVSLSVPYYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRN
NRYQAKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGGSLNAGLSVLKYQTLANQS
GYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSRQDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFG
SQEARRVSRTLPIVNIDSGMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYGNDR
INTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRSDWVAFASSGIGSRFILDSSIHYNQND
KRAENYAVGASYRPAQGKVLNARYKYGRNEKIYLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLA
GAEYKSSCGCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGRNKRP
>Mature_792_residues
MLALGLCFGTHCAAADAVAAEETDNPTAGGSVRSVSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYT
RIVADRMEGQSQVQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQQTGEAHNVRM
ETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASVEADREKGIGVAKHAAFVFGGVPIFYTPWADFP
LDGNRKSGLLVPSLSAGSDGVSLSVPYYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRN
NRYQAKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGGSLNAGLSVLKYQTLANQS
GYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSRQDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFG
SQEARRVSRTLPIVNIDSGMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYGNDR
INTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRSDWVAFASSGIGSRFILDSSIHYNQND
KRAENYAVGASYRPAQGKVLNARYKYGRNEKIYLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLA
GAEYKSSCGCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGRNKRP

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=767, Percent_Identity=27.7705345501956, Blast_Score=247, Evalue=3e-66,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 87347; Mature: 87347

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALGLCFGTHCAAADAVAAEETDNPTAGGSVRSVSEPIQPTSLSLGSTCLFCSNESGSP
CEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCC
ERTEAAVQGSGEASIPEDYTRIVADRMEGQSQVQVRAEGNVVVERNRTTLNADWADYDQS
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEECCEEEECCCCCCCCC
GDTVTAGDRFALQQDGTLIRGETLTYNLEQQTGEAHNVRMETEHGGRRLQSVSRTAEMLG
CCEEECCCEEEEECCCCEEECCEEEEEEHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHC
EGHYKLTETQFNTCSAGDAGWYVKAASVEADREKGIGVAKHAAFVFGGVPIFYTPWADFP
CCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHCCCHHHHHEEEECCCCEEECCCCCCC
LDGNRKSGLLVPSLSAGSDGVSLSVPYYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRP
CCCCCCCCEEEECCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCEECCEEEEECC
DYAGQSDLTWLPHDKKSGRNNRYQAKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKE
CCCCCCCCEECCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCHHHCCCCCCEEEECCCCE
IAGNVNLNRRVWLDYGGRAAGGSLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKN
EEECCCCCEEEEEECCCCCCCCCCCCCHHHEEEHHHHCCCCCCCCCEEECCCCCCCHHCC
TGRAQIGVSAQFTRFSHDSRQDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFG
CCCEEEECEEEEEECCCCCCCCCCEEEEECCEEEECCCCCCEECCCCCCEEEEEEHHHCC
SQEARRVSRTLPIVNIDSGMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSS
CHHHHHHHHHCCEEECCCCCEEECCCEEECCCHHHHCCCEEEEEECCCCCCCCCCCCCCC
ESSFGYGQLFRENLYYGNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAV
CCCCCHHHHHHHHCEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCE
MLDGSVGKKPRSRSDWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVL
EEECCCCCCCCCCCCEEEEECCCCCCEEEEECCCEECCCCCCCCCEEECCCCCCCCCCEE
NARYKYGRNEKIYLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLA
EEEHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHEEE
GAEYKSSCGCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIP
CCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHHCCCCCHHCEEECCCCCC
AHSLSAGRNKRP
CCCCCCCCCCCC
>Mature Secondary Structure
MLALGLCFGTHCAAADAVAAEETDNPTAGGSVRSVSEPIQPTSLSLGSTCLFCSNESGSP
CEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCC
ERTEAAVQGSGEASIPEDYTRIVADRMEGQSQVQVRAEGNVVVERNRTTLNADWADYDQS
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEECCEEEECCCCCCCCC
GDTVTAGDRFALQQDGTLIRGETLTYNLEQQTGEAHNVRMETEHGGRRLQSVSRTAEMLG
CCEEECCCEEEEECCCCEEECCEEEEEEHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHC
EGHYKLTETQFNTCSAGDAGWYVKAASVEADREKGIGVAKHAAFVFGGVPIFYTPWADFP
CCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHCCCHHHHHEEEECCCCEEECCCCCCC
LDGNRKSGLLVPSLSAGSDGVSLSVPYYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRP
CCCCCCCCEEEECCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCEECCEEEEECC
DYAGQSDLTWLPHDKKSGRNNRYQAKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKE
CCCCCCCCEECCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCHHHCCCCCCEEEECCCCE
IAGNVNLNRRVWLDYGGRAAGGSLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKN
EEECCCCCEEEEEECCCCCCCCCCCCCHHHEEEHHHHCCCCCCCCCEEECCCCCCCHHCC
TGRAQIGVSAQFTRFSHDSRQDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFG
CCCEEEECEEEEEECCCCCCCCCCEEEEECCEEEECCCCCCEECCCCCCEEEEEEHHHCC
SQEARRVSRTLPIVNIDSGMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSS
CHHHHHHHHHCCEEECCCCCEEECCCEEECCCHHHHCCCEEEEEECCCCCCCCCCCCCCC
ESSFGYGQLFRENLYYGNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAV
CCCCCHHHHHHHHCEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCE
MLDGSVGKKPRSRSDWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVL
EEECCCCCCCCCCCCEEEEECCCCCCEEEEECCCEECCCCCCCCCEEECCCCCCCCCCEE
NARYKYGRNEKIYLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLA
EEEHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHEEE
GAEYKSSCGCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIP
CCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHHCCCCCHHCEEECCCCCC
AHSLSAGRNKRP
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]