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The map label for this gene is purB [H]

Identifier: 161870779

GI number: 161870779

Start: 1906715

End: 1908085

Strand: Reverse

Name: purB [H]

Synonym: NMCC_1861

Alternate gene names: 161870779

Gene position: 1908085-1906715 (Counterclockwise)

Preceding gene: 161870780

Following gene: 161870778

Centisome position: 88.61

GC content: 55.0

Gene sequence:

>1371_bases
ATGATCAACCCCATCGCCTCGCTTTCCCCTTTAGACGGCCGTTATGCCCAATCCGTTGAAGCATTGCGCCCGATTTTTTC
CGAATACGGCCTGATGAAAGCGCGCGTCAAAGTCGAATTAAACTGGCTCAAAGCCCTCGCCGCCGAGCCGAAGATTGCCG
AAGTGCCGCCCTTCAGTGCCGAAACGCTTGCCGAAATCGACACGGTGATTGAAAACTTTTCATTGGAAGACGCGGCCGCC
GTCAAAGCCATCGAAGCCACGACCAATCATGATGTCAAAGCCATTGAATATTGGCTGAAAAAACGTTTTGCCGAAGTGCC
GGAAGTCGCCGCCGTGAGTGAGTTCATCCACTTCGCCTGCACCAGCGAAGACATCAACAACCTGTCCCACGCTTTAATGC
TGCAAGAAGCGCGTGAGGCTGTTTTGCTGCCGAAGCTGGCCGAAATCATCGAAAAACTGACCGCTATGGCGCACGACCTT
GCCGCCGTCCCGATGATGAGCCGCACCCACGGCCAGCCCGCCACGCCGACCACTTTGGGCAAAGAAACCGCCAATGTCGT
GTACCGCCTGCAACGCCAGTTTAAAAACCTGCAAGCGCAAGAGTTCCTCGGCAAAATCAACGGCGCGGTCGGCAATTACA
ACGCTCACATGGTCGTCTATCCCGACGTCGATTGGGAAACCCACTGCCGTAACTTCGTTGAAATCAGCCTCGGCCTGACC
TTCAACCCCTACACCATCCAAATCGAGCCGCACGACTACATGGCTGAATTCTTCCAAGCCATCAGCCGTGTCAACACGAT
TCTGATCGACTTCAACCGCGATGTTTGGGGCTACATTTCTTTGGGTTACTTCAAACAAAAAGTCAAAGCAGGCGAAGTCG
GTTCTTCCACCATGCCGCACAAAGTCAACCCCATTGACTTTGAAAACTCCGAGGGCAACCTCGGTATGGCCAACGCCGTA
TTGGGCTTTTTGTCCGAAAAACTGCCGATTTCCCGTTGGCAGCGCGACCTGACCGACAGCACCGTCCTGCGCAATATGGG
CGTGGGCGTGGGCTATGCCGTGTTGGGTTTCGCCGCCCACTTGCGCGGTCTGAACAAGCTTGAACCGAATCCTGCCGCCC
TTGCCGCCGATTTGGACGCTACTTGGGAGCTGCTCGCCGAGCCGATTCAAACCGTAATGCGTCGTTACGGCGTGGCCAAT
CCTTACGAGAAGCTGAAAGACCTGACGCGCGGTAAAGACGGCATTACGCCCGAAGTGCTGAAAGTCTTCATCGAATCGCT
GGAAATCCCTACCGAAGCCAAAGCAAAATTGCTTGAGCTGACCCCCGCACTGTATGTGGGCAAGGCTGAAGCGTTGGCGA
AACGGATTTGA

Upstream 100 bases:

>100_bases
TGTCGGAACACGGGCTTCAGACGGCATAGCGTCCTGAGAGTGATGTATAATTGCAAACAATTTAACAATTTTTGATGTCT
TTCCCGAACAGGATGCCGAA

Downstream 100 bases:

>100_bases
GCGTTTACTGAAACCGATGCCGTCTGAACGCGCGTTCAGACGGCATTTTTAAAATACCGGATATACAGGGGCGATATTGA
TGCAAGCTGTCCGATACAGG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLKALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAA
VKAIEATTNHDVKAIEYWLKKRFAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMVVYPDVDWETHCRNFVEISLGLT
FNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVWGYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAV
LGFLSEKLPISRWQRDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTVMRRYGVAN
PYEKLKDLTRGKDGITPEVLKVFIESLEIPTEAKAKLLELTPALYVGKAEALAKRI

Sequences:

>Translated_456_residues
MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLKALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAA
VKAIEATTNHDVKAIEYWLKKRFAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMVVYPDVDWETHCRNFVEISLGLT
FNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVWGYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAV
LGFLSEKLPISRWQRDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTVMRRYGVAN
PYEKLKDLTRGKDGITPEVLKVFIESLEIPTEAKAKLLELTPALYVGKAEALAKRI
>Mature_456_residues
MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLKALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAA
VKAIEATTNHDVKAIEYWLKKRFAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMVVYPDVDWETHCRNFVEISLGLT
FNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVWGYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAV
LGFLSEKLPISRWQRDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTVMRRYGVAN
PYEKLKDLTRGKDGITPEVLKVFIESLEIPTEAKAKLLELTPALYVGKAEALAKRI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=448, Percent_Identity=61.8303571428571, Blast_Score=598, Evalue=1e-172,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50596; Mature: 50596

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLKALAAEPKIAEVPPFSA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHEEEEHHHHHHHHCCCCCCCCCCCCH
ETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKRFAEVPEVAAVSEFIHFAC
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
TSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDLAAVPMMSRTHGQPATPTTLG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
KETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMVVYPDVDWETHCRNFVEISLGLT
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCEEEEEEEEE
FNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVWGYISLGYFKQKVKAGEVGSSTMPH
ECCEEEEECCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCC
KVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQRDLTDSTVLRNMGVGVGYAVLGFAAH
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LRGLNKLEPNPAALAADLDATWELLAEPIQTVMRRYGVANPYEKLKDLTRGKDGITPEVL
HCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
KVFIESLEIPTEAKAKLLELTPALYVGKAEALAKRI
HHHHHHHCCCCHHHHHHHHHCHHHHHCCHHHHHHCC
>Mature Secondary Structure
MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLKALAAEPKIAEVPPFSA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHEEEEHHHHHHHHCCCCCCCCCCCCH
ETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKRFAEVPEVAAVSEFIHFAC
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
TSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDLAAVPMMSRTHGQPATPTTLG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
KETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMVVYPDVDWETHCRNFVEISLGLT
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCEEEEEEEEE
FNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVWGYISLGYFKQKVKAGEVGSSTMPH
ECCEEEEECCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCC
KVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQRDLTDSTVLRNMGVGVGYAVLGFAAH
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LRGLNKLEPNPAALAADLDATWELLAEPIQTVMRRYGVANPYEKLKDLTRGKDGITPEVL
HCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
KVFIESLEIPTEAKAKLLELTPALYVGKAEALAKRI
HHHHHHHCCCCHHHHHHHHHCHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]