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The map label for this gene is ispB [H]

Identifier: 161870743

GI number: 161870743

Start: 1862360

End: 1863334

Strand: Reverse

Name: ispB [H]

Synonym: NMCC_1817

Alternate gene names: 161870743

Gene position: 1863334-1862360 (Counterclockwise)

Preceding gene: 161870748

Following gene: 161870742

Centisome position: 86.53

GC content: 51.49

Gene sequence:

>975_bases
ATGCTCGAGAATCTGCCCTATTTCCAGCGACATCTGCCTGAAGACCTTGCCAAAGTCAATGAAGTCATCAACCGTGCGGT
GCAATCCGATGTCGCACTGATTTCGCAAATCGGTACATATATCATCAGCGCGGGCGGCAAACGCCTGCGTCCGATTATGA
CGATTTTGGCGGGTAAGGCGGTCGGTTATGATGACGAGAAACTGTATTCGCTGGCGGCGATGGTCGAGTTTATCCACACT
TCCACCCTCCTGCACGACGATGTCGTCGATGAAAGCGATTTGCGCCGTGGGCGGGCAACGGCAAACAATCTGTTCGGCAA
TGCGGCGGCTGTGTTGGTTGGCGACTTTTTATACACGCGCGCCTTTCAACTGATGGTTGCCTCGGGCAGTATGCGCGTTT
TGGAAGTGATGGCGGATGCAACCAACATTATTGCCGAGGGCGAAGTCATGCAGCTGATGAACATCGGCAATACGGACATT
ACCGAAGAACAATATATCCAAGTCATCCAATATAAAACGGCAAAATTGTTTGAAGCTGCCGCTCAAGTCGGCGCAATTTT
GGGCAAGGCTTCCCCCGAACACGAACGGGCGTTGAAAGACTACGGTATGTATGTCGGTACGGCATTCCAAATTATTGACG
ATGTGCTGGACTATTCTGGCGAAACCGAAGAAACCGGCAAAAACGTCGGCGACGATTTGGCGGAAGGAAAACCGACTTTG
CCTTTGATTTATCTGATGCGTCAGGGTTCCGAACAGGTTGCGAACGATGTGCGTACTGCTTTGGAAAATGCAGATCGCAG
CTATTTTGAGAAAATCCACGATTATGTCGTCCGTTCGGATGCGTTGGCATATTCGATAGGCGAGGCGCGCAAAGCAGTCG
ATTGTGCCGTTACCGCCTTGGATGCCCTGCCCGACAGCGAAGTGAAGGATGCCATGATTCAGCTGGCGAAGGAATCTTTG
GTCAGGGTGTCTTGA

Upstream 100 bases:

>100_bases
GATGTACCCTGATGGCTTTGCTGATATAATTGCCCGCTATTTGAATCAGCTTTCAAGCGGTATCTGCCGTTTGACGGAAA
CGTAAACCTGAGAGTCTGCC

Downstream 100 bases:

>100_bases
GGCGATGAATGTCAGTTTTGCTCCCCTGTTTCTGGTTACGCTGATTCTGTTGGGGGTGGTCAGCAACAACAATTCGATTA
CCATCTCGGCAACCATATTG

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISAGGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHT
STLLHDDVVDESDLRRGRATANNLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDYSGETEETGKNVGDDLAEGKPTL
PLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYVVRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESL
VRVS

Sequences:

>Translated_324_residues
MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISAGGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHT
STLLHDDVVDESDLRRGRATANNLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDYSGETEETGKNVGDDLAEGKPTL
PLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYVVRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESL
VRVS
>Mature_324_residues
MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISAGGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHT
STLLHDDVVDESDLRRGRATANNLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDYSGETEETGKNVGDDLAEGKPTL
PLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYVVRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESL
VRVS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=317, Percent_Identity=29.3375394321767, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI4758430, Length=229, Percent_Identity=25.3275109170306, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI83700220, Length=229, Percent_Identity=25.3275109170306, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789578, Length=320, Percent_Identity=53.4375, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1786623, Length=299, Percent_Identity=29.4314381270903, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17505681, Length=353, Percent_Identity=32.0113314447592, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319475, Length=301, Percent_Identity=31.5614617940199, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=242, Percent_Identity=26.8595041322314, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24651612, Length=251, Percent_Identity=30.6772908366534, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI281365769, Length=233, Percent_Identity=25.3218884120172, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24660002, Length=233, Percent_Identity=25.3218884120172, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35538; Mature: 35538

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISAGGKRLRPIMTILAGKA
CCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
VGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATANNLFGNAAAVLVGDFLYTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDITEEQYIQVIQYKTAKLFEAA
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDYSGETEETGKNVGDDLAEGKPTL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHCCCCCC
PLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYVVRSDALAYSIGEARKAVDCAVTAL
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALPDSEVKDAMIQLAKESLVRVS
HCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISAGGKRLRPIMTILAGKA
CCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
VGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATANNLFGNAAAVLVGDFLYTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDITEEQYIQVIQYKTAKLFEAA
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDYSGETEETGKNVGDDLAEGKPTL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHCCCCCC
PLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYVVRSDALAYSIGEARKAVDCAVTAL
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALPDSEVKDAMIQLAKESLVRVS
HCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]