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The map label for this gene is murF [H]

Identifier: 161870658

GI number: 161870658

Start: 1771985

End: 1773376

Strand: Reverse

Name: murF [H]

Synonym: NMCC_1728

Alternate gene names: 161870658

Gene position: 1773376-1771985 (Counterclockwise)

Preceding gene: 161870659

Following gene: 161870657

Centisome position: 82.35

GC content: 55.82

Gene sequence:

>1392_bases
GTGTCCGCCGCCCGAATATTAACGAAAGTAAATATGAAACCACTGGACCTAAATTTCATCTGCCAAGCCCTCAAGCTTCC
GATGCCGTCTGAAAGCAAACCCGTGTCGCGCATCGTAACCGACAGCCGCGACATCCGCGTAGGCGATGTGTTTTTCGCAT
TGGCGGGCGGGCGGTTTGACGCGCATGATTTTGTTGAAGACGTATTGGCTGCGGGTGCGGCGGCGGTTGTGGTTTCGCGC
GAAGATTGCGTTGCAATGGATGGCGCGTTGAAAGTCGATGACACGCTTGCCGCATTGCAAACGCTGGCAAAGGCGTGGCG
CGAGAATGTGAACCCGTTTGTGTTCGGTATTACCGGCTCGGGCGGTAAGACGACGGTGAAGGAAATGTTGGCTGCGGTAT
TGCGCCGCCGTTTCGGCGATAATGCCGTTTTGGCGACGGCAGGCAACTTCAACAACCACATCGGATTGCCGTTGACTTTG
TTGAAATTAAACGAAAAACACCGCTATGCCGTGATTGAAATGGGTATGAACCATTTTGGCGAACTGGCGGTTTTGACACA
AATCGCCAAACCCGATGCCGCATTGGTCAACAACGCCATGCGCGCCCATGTCGGCTGCGGTTTCGACGGAGTGGGCGATA
TTGCCAAAGCGAAAAGCGAGATTTATCAAGGCTTATGTTCAGACGGCATGGCGCTGATTCCTCAAGAAGATGCCAATATG
GCTGTCTTCAAAACGGCAACGCTTAATTTGAATACGCGCACTTTCGGCATCGATAGCGGCGATGTCCACGCGGAAAATAT
CGTGCTGAAACCGTTGTCGTGCGAATTTGATTTGGTGTGCGGCGACGAACGCGCCGCCGTGGTGCTGCCTGTTCCCGGCC
GCCACAATGTCCACAACGCCGCCGCTGCCGCCGCGCTGTCTTTGGCTGCGGGTTTGAGTCTGAACGATGTGGCGGAAGGT
TTGCAAGGCTTCAGCAACATCAAAGGCCGTCTGAACGTCAAAGCCGGCATCAAGGGCGCGACCCTGATTGACGATACCTA
CAACGCGAACCCCGACAGCATGAGGGCAGCGATTGACGTGTTGGCGCGTATGCCTGCGCCGCGCATTTTTGTGATGGGCG
ATATGGGCGAACTGGGTGAGGACGAAGCCGCCGCCATGCACGCCGAAGTCGGCGCGTACGCCCGCGACCAAGGCATCGAA
GCGGCTTATTTTGTCGGAGACAACAGCGTCGAAGCGGCGGAAAAATTTGGCGCGGACGGTTTGTGGTTCGCCGCCAAAGA
CCCGTTGATTCAAGTGTTGCGCCACGATTTGCCCGAACGCGCCACCGTGTTGGTGAAAGGTTCGCGCTTTATGCAGATGG
AAGAAGTGGTCGAGGCATTGGAGGATAAGTGA

Upstream 100 bases:

>100_bases
AAAAAATGATACGTACAGAAAAAATGCCGAATGAGAATGGGAAAATAATCTGTGTTTTACCACAGCAAAACAGGCGATAA
AAAAATCAGCCGCTGCCGAT

Downstream 100 bases:

>100_bases
AAATGAAAAGTCGACGTTTTTTTAAAGCCTTATTGTTGATTGCCGCGCTGGTCGGCGCGTTTTATGCCGGAATGCAGACG
CAGGCGTATCTTTATGAAGA

Product: UDP-MurNAc-pentapeptide synthetase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSAARILTKVNMKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRVGDVFFALAGGRFDAHDFVEDVLAAGAAAVVVSR
EDCVAMDGALKVDDTLAALQTLAKAWRENVNPFVFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTL
LKLNEKHRYAVIEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDGMALIPQEDANM
AVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAVVLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEG
LQGFSNIKGRLNVKAGIKGATLIDDTYNANPDSMRAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIE
AAYFVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALEDK

Sequences:

>Translated_463_residues
MSAARILTKVNMKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRVGDVFFALAGGRFDAHDFVEDVLAAGAAAVVVSR
EDCVAMDGALKVDDTLAALQTLAKAWRENVNPFVFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTL
LKLNEKHRYAVIEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDGMALIPQEDANM
AVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAVVLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEG
LQGFSNIKGRLNVKAGIKGATLIDDTYNANPDSMRAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIE
AAYFVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALEDK
>Mature_462_residues
SAARILTKVNMKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRVGDVFFALAGGRFDAHDFVEDVLAAGAAAVVVSRE
DCVAMDGALKVDDTLAALQTLAKAWRENVNPFVFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLL
KLNEKHRYAVIEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDGMALIPQEDANMA
VFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAVVLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGL
QGFSNIKGRLNVKAGIKGATLIDDTYNANPDSMRAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEA
AYFVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALEDK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=429, Percent_Identity=42.1911421911422, Blast_Score=255, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49346; Mature: 49215

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARILTKVNMKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRVGDVFFALAGGRFD
CCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEEEEEEEECCCCC
AHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLAALQTLAKAWRENVNPFVFGITGS
HHHHHHHHHHCCCEEEEEECCCCEEECCCEECHHHHHHHHHHHHHHHCCCCEEEEEEECC
GGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAVIEMGMNHFG
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHH
ELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDGMALIPQEDANM
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCE
AVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAVVLPVPGRHNVHNA
EEEEEEEEEECCEEECCCCCCCEECEEEEEECCCCEEEEECCCCCEEEEECCCCCCCHHH
AAAAALSLAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDDTYNANPDSMRAAIDV
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
LARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAYFVGDNSVEAAEKFGADG
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHCCCCEEEEEECCCCHHHHHHCCCCC
LWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALEDK
EEEECCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAARILTKVNMKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRVGDVFFALAGGRFD
CHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEEEEEEEECCCCC
AHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLAALQTLAKAWRENVNPFVFGITGS
HHHHHHHHHHCCCEEEEEECCCCEEECCCEECHHHHHHHHHHHHHHHCCCCEEEEEEECC
GGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAVIEMGMNHFG
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHH
ELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDGMALIPQEDANM
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCE
AVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAVVLPVPGRHNVHNA
EEEEEEEEEECCEEECCCCCCCEECEEEEEECCCCEEEEECCCCCEEEEECCCCCCCHHH
AAAAALSLAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDDTYNANPDSMRAAIDV
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
LARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAYFVGDNSVEAAEKFGADG
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHCCCCEEEEEECCCCHHHHHHCCCCC
LWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALEDK
EEEECCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]