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The map label for this gene is murD [H]

Identifier: 161870654

GI number: 161870654

Start: 1768668

End: 1770005

Strand: Reverse

Name: murD [H]

Synonym: NMCC_1724

Alternate gene names: 161870654

Gene position: 1770005-1768668 (Counterclockwise)

Preceding gene: 161870655

Following gene: 161870653

Centisome position: 82.2

GC content: 56.5

Gene sequence:

>1338_bases
ATGACTTTCCAAAACAAAAAAATCCTCGTCGCCGGACTTGGCGGCACGGGTATTTCCATGATTGCCTACCTGCGCAAAAA
CGGCGTGGAGGTTGCCGCTTATGATGCGGAGTTGAAGCCCGAGCGCGTGGCGCAAATCGGTAAGATGTTTGACGGGCTGG
TGTTTTACACAGGCCGTCTGAAAGATGCGCTGGACAACGGTTTTGATATTCTGGCGCTCAGCCCCGGCATCAGCGAGCGG
CAGCCGGATATCGAGGCGTTCAAGCAAAACGGCGGGCGCGTGTTGGGTGATATCGAATTGCTGGCGGATATTGTGAACCG
CCGAGGCGACAAGGTGATTGCGATTACCGGCAGCAACGGCAAAACCACGGTAACGAGCCTGGTCGGCTATCTCTGCATCA
AGTGCGGGCTGGATACCGTTATCGCGGGCAATATCGGCACGCCGGTTTTGGAGGCGGAATGGCAGCGCGAAGGCAAAAAG
GCGGACGTGTGGGTGTTGGAGCTTTCCAGCTTCCAACTGGAAAACACCGAAAGCCTGCGCCCGACTGCGGCGACGGTGCT
GAACATTTCCGAAGACCATCTCGACCGCTACGACGACTTGCTCGACTATGCGCATACCAAGGATAAGATTTTCCGTGGCG
ATGGCGTGCAGGTTTTGAATGCGGACGATGCGTTCTGTCGTGCGATGAAGCGCGCCGGGCGCGAGGTGAAATGGTTTTCG
TTGGAATACGAAGCCGATTTTTGGTTGGAACGTGAGACAGGTCGCCTGAAACAAGGCAATGAAGATTTGATTGCCACGCA
AGATATTCCGCTGCAAGGCTTGCACAACGCCACTAACGTGATGGCTGCCGTTGCCTTGTGCGAAGCCGTCGGTTTGCCGC
GCGAAGCATTGCTCGAACACGTCAAAACCTTTCAAGGCCTGCCGCACCGCGTGGAAAAAATCGGCGAGAAAAATGGCGTG
GTGTTCATCGACGACAGCAAAGGCACGAACGTCGGCGCGACAGCCGCCGCGATTGCCGGTTTGCAAAATCCGCTATTCGT
CATTTTGGGCGGCATGGGCAAAGGGCAGGACTTCACGCCCCTGCGCGACGCGCTTGCCGGCAAGGCAAAAGGCGTGTTCC
TGATTGGTGTCGATGCGCTGCAAATCCGCCGCGATTTGGACGGCTGCGATCTGAATATGACCGACTGCGCCACTTTGGAA
GAAGCGGTTCAGAAGGCATATGCCCAAGCCGAAGCGGGCGATATCGTGCTGCTCAGCCCCGCCTGCGCGAGCTTCGATAT
GTTTAAAGGCTATGCGCACCGTTCGGAAGTGTTTATCGGGGCGTTTAAGGCTTTGTGA

Upstream 100 bases:

>100_bases
GAAAGCGGGTTATTGAACAGCTCGCGATGCCGTCTGAAAGCGGCTTGAGCAGGAGCAGACATCTTCCTGCCCAATATGAT
TTGTTTTAGGACAAACCAAA

Downstream 100 bases:

>100_bases
TACCGTCTGAAACGCAAACGCCGTCATTGTTGGGCGGCAAGTAAAGATTTAGAATACCGATTTGGGATGTATCGTATGTT
CGGACGGCATTGTCTGCCGT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTFQNKKILVAGLGGTGISMIAYLRKNGVEVAAYDAELKPERVAQIGKMFDGLVFYTGRLKDALDNGFDILALSPGISER
QPDIEAFKQNGGRVLGDIELLADIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQVLNADDAFCRAMKRAGREVKWFS
LEYEADFWLERETGRLKQGNEDLIATQDIPLQGLHNATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGV
VFIDDSKGTNVGATAAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDALQIRRDLDGCDLNMTDCATLE
EAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL

Sequences:

>Translated_445_residues
MTFQNKKILVAGLGGTGISMIAYLRKNGVEVAAYDAELKPERVAQIGKMFDGLVFYTGRLKDALDNGFDILALSPGISER
QPDIEAFKQNGGRVLGDIELLADIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQVLNADDAFCRAMKRAGREVKWFS
LEYEADFWLERETGRLKQGNEDLIATQDIPLQGLHNATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGV
VFIDDSKGTNVGATAAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDALQIRRDLDGCDLNMTDCATLE
EAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL
>Mature_444_residues
TFQNKKILVAGLGGTGISMIAYLRKNGVEVAAYDAELKPERVAQIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQ
PDIEAFKQNGGRVLGDIELLADIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKKA
DVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQVLNADDAFCRAMKRAGREVKWFSL
EYEADFWLERETGRLKQGNEDLIATQDIPLQGLHNATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVV
FIDDSKGTNVGATAAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDALQIRRDLDGCDLNMTDCATLEE
AVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=444, Percent_Identity=38.0630630630631, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1786274, Length=259, Percent_Identity=30.1158301158301, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48259; Mature: 48128

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFQNKKILVAGLGGTGISMIAYLRKNGVEVAAYDAELKPERVAQIGKMFDGLVFYTGRL
CCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECHH
KDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLADIVNRRGDKVIAITGSNG
HHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCC
KTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKKADVWVLELSSFQLENTESLR
CHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCCCCCEEEEEECCEEECCCCCCC
PTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQVLNADDAFCRAMKRAGREVKWFS
CCCCEEEECCHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHHHHHCCCEEEEEE
LEYEADFWLERETGRLKQGNEDLIATQDIPLQGLHNATNVMAAVALCEAVGLPREALLEH
EEECCCEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGATAAAIAGLQNPLFVILGGMGKGQDFTP
HHHHCCCCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCH
LRDALAGKAKGVFLIGVDALQIRRDLDGCDLNMTDCATLEEAVQKAYAQAEAGDIVLLSP
HHHHHCCCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECC
ACASFDMFKGYAHRSEVFIGAFKAL
CCCCHHHHHHHCCCCCEEEEHHHCC
>Mature Secondary Structure 
TFQNKKILVAGLGGTGISMIAYLRKNGVEVAAYDAELKPERVAQIGKMFDGLVFYTGRL
CCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECHH
KDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLADIVNRRGDKVIAITGSNG
HHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCC
KTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKKADVWVLELSSFQLENTESLR
CHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCCCCCEEEEEECCEEECCCCCCC
PTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQVLNADDAFCRAMKRAGREVKWFS
CCCCEEEECCHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHHHHHCCCEEEEEE
LEYEADFWLERETGRLKQGNEDLIATQDIPLQGLHNATNVMAAVALCEAVGLPREALLEH
EEECCCEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGATAAAIAGLQNPLFVILGGMGKGQDFTP
HHHHCCCCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCH
LRDALAGKAKGVFLIGVDALQIRRDLDGCDLNMTDCATLEEAVQKAYAQAEAGDIVLLSP
HHHHHCCCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECC
ACASFDMFKGYAHRSEVFIGAFKAL
CCCCHHHHHHHCCCCCEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA