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The map label for this gene is ddl [H]

Identifier: 161870650

GI number: 161870650

Start: 1763556

End: 1764440

Strand: Reverse

Name: ddl [H]

Synonym: NMCC_1720

Alternate gene names: 161870650

Gene position: 1764440-1763556 (Counterclockwise)

Preceding gene: 229597116

Following gene: 161870649

Centisome position: 81.94

GC content: 53.56

Gene sequence:

>885_bases
ATGGGCGGTTTTTCCAGCGAACGAGAAATCTCGCTGGACAGCGGCACCGCCATTTTGAATGCTTTAAAAAGCAAAGGTAT
AGACGCATACGCCTTCGATCCCAAGGAAACCCCATTGTCTGAATTGAAGGCACAAGGTTTTCAGACGGCATTCAACATCC
TTCACGGTACTTACGGCGAAGACGGGGCGGTTCAAGGCGCGTTGGAACTGTTGGGCATTCCCTATACCGGCAGCGGTGTC
GCCGCCTCCGCCATCGGCATGGACAAATACCGCTGCAAACTGATTTGGCAGGCATTGGGGCTGCCCGTTCCCGAGTTCGC
CGTCCTGCACGACGACACTGATTTCGATGCCGTCGAAGAAAAATTGGGCCTGCCGATGTTTGTGAAACCGGCGGCCGAAG
GCAGCAGCGTAGGCGTGGTAAAAGTCAAAGGAAAAGGCCGTCTGAAAAGCGTTTACGAAGAATTGAAACACCTTCAGGGC
GAAATCATTGCCGAACGGTTTATCGGCGGCGGCGAATATTCCTGCCCCGTCCTGAACGGCAAAGGGCTGCCCGGCATACA
CATCATTCCCGCAACCGAGTTTTACGACTACGAAGCCAAGTACAACCGCGACGACACCATTTATCAATGTCCTTCGGAAG
ATTTGACCGAAGCCGAAGAAAGCCTGATGCGCGAACTGGCGGTTCGCGGGGCGCAGGCAATCGGTGCGGAAAGCTGCGTG
CGCGTCGATTTCCTCAAAGATACCGACGGCAAACTCTATCTGTTGGAAATCAACACCCTGCCCGGTATGACGAGCCATAG
TTTAGTACCGAAATCCGCTGCCGTTACGGGCGTGGGTTTTGCCGATTTATGTATTGAAATTTTGAAGACCGCACATGTGG
GATAA

Upstream 100 bases:

>100_bases
TGAAACCGCAAATCAGGTTTCAGACGACCTCTGGCAACAAGCATAAAGCAATCAGGAAAGAACAAAAACAATGCAGAATT
TTGGCAAAGTGGCCGTATTG

Downstream 100 bases:

>100_bases
TGCCGAAGCGATGGAACGGCTGACGCGCTGGCTGCTTGTCATGATGGCGATGCTGCTTGCTGCGTCCGGGCTGGTTTGGT
TTTACAATTCGAATCATCTG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MGGFSSEREISLDSGTAILNALKSKGIDAYAFDPKETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGV
AASAIGMDKYRCKLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKSVYEELKHLQG
EIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIYQCPSEDLTEAEESLMRELAVRGAQAIGAESCV
RVDFLKDTDGKLYLLEINTLPGMTSHSLVPKSAAVTGVGFADLCIEILKTAHVG

Sequences:

>Translated_294_residues
MGGFSSEREISLDSGTAILNALKSKGIDAYAFDPKETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGV
AASAIGMDKYRCKLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKSVYEELKHLQG
EIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIYQCPSEDLTEAEESLMRELAVRGAQAIGAESCV
RVDFLKDTDGKLYLLEINTLPGMTSHSLVPKSAAVTGVGFADLCIEILKTAHVG
>Mature_293_residues
GGFSSEREISLDSGTAILNALKSKGIDAYAFDPKETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVA
ASAIGMDKYRCKLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKSVYEELKHLQGE
IIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIYQCPSEDLTEAEESLMRELAVRGAQAIGAESCVR
VDFLKDTDGKLYLLEINTLPGMTSHSLVPKSAAVTGVGFADLCIEILKTAHVG

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=298, Percent_Identity=45.6375838926174, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI1786579, Length=342, Percent_Identity=32.7485380116959, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 31496; Mature: 31365

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGFSSEREISLDSGTAILNALKSKGIDAYAFDPKETPLSELKAQGFQTAFNILHGTYGE
CCCCCCCCEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
DGAVQGALELLGIPYTGSGVAASAIGMDKYRCKLIWQALGLPVPEFAVLHDDTDFDAVEE
CCHHHHHHHHEECCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
KLGLPMFVKPAAEGSSVGVVKVKGKGRLKSVYEELKHLQGEIIAERFIGGGEYSCPVLNG
HCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
KGLPGIHIIPATEFYDYEAKYNRDDTIYQCPSEDLTEAEESLMRELAVRGAQAIGAESCV
CCCCCEEEEECCCEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
RVDFLKDTDGKLYLLEINTLPGMTSHSLVPKSAAVTGVGFADLCIEILKTAHVG
EEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GGFSSEREISLDSGTAILNALKSKGIDAYAFDPKETPLSELKAQGFQTAFNILHGTYGE
CCCCCCCEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
DGAVQGALELLGIPYTGSGVAASAIGMDKYRCKLIWQALGLPVPEFAVLHDDTDFDAVEE
CCHHHHHHHHEECCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
KLGLPMFVKPAAEGSSVGVVKVKGKGRLKSVYEELKHLQGEIIAERFIGGGEYSCPVLNG
HCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
KGLPGIHIIPATEFYDYEAKYNRDDTIYQCPSEDLTEAEESLMRELAVRGAQAIGAESCV
CCCCCEEEEECCCEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
RVDFLKDTDGKLYLLEINTLPGMTSHSLVPKSAAVTGVGFADLCIEILKTAHVG
EEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA