Click here to switch to the map view.

The map label for this gene is bcr [H]

Identifier: 161870629

GI number: 161870629

Start: 1734435

End: 1735688

Strand: Reverse

Name: bcr [H]

Synonym: NMCC_1698

Alternate gene names: 161870629

Gene position: 1735688-1734435 (Counterclockwise)

Preceding gene: 161870630

Following gene: 161870628

Centisome position: 80.6

GC content: 56.7

Gene sequence:

>1254_bases
TTGCGAATCGGACGAGAATTTATGCCTTCTGCCCATTATCCTGAAATGAGCGAAAAACTGATGGCGGTTTTGATGGCGAT
GCTGGTTACGCTGATGCCGTTTTCCATCGATGCCTACCTGCCCGCGATTCCCGAAATGGCGCAATCGCTGAACGCGGATG
TTCACCGCATCGAACAGAGTTTGAGTTTGTTTATGTTCGGCACGGCGTTCGGACAGGTGGTCGGCGGTTCGGTGTCCGAC
ATCAAAGGGCGCAAACCCGTCGCCCTGACCGGTTTGATTGTATATTGCCTTGCCGTTGCCGCCATCGTATTTGTTTCGAG
TGCCGAACAGCTCCTCAACCTGCGCGTCGTGCAGGCATTCGGTGCGGGCATGACTGTGGTCATCGTCGGCGCAATGGTGC
GCGATTATTATTCCGGACGCAAAGCCGCCCAGATGTTTGCCCTTATCGGCATCATTTTGATGGTTGTGCCGCTGGTCGCA
CCCATGGTCGGCGCATTGTTGCAGGGCTTGGGTGGCTGGCAGGCGATTTTTGTTTTTCTGGCGGCGTATTCGCTGGTGCT
GCTCGGTTTGGTACAGTATTTCCTGCCCAAGCCCGCCGTCGGCGGCAAAATCGGACGGGACGTGTTCGGGCTGGTGGCGG
GGCGGTTCAAGCGCGTATTGAAAACCCGTGCCGCGATGGGTTATCTGTTTTTTCAGGCATTCAGCTTCGGTTCGATGTTC
GCCTTTCTGACCGAATCTTCCTTCGTGTACCAGCAGCTTTACCGTGTTACGCCTCATCAATACGCTTGGGCGTTTGCACT
CAACATCATCACGATGATGTTTTTCAACCGCGTTACCGCGTGGCGGCTCAAAACCGGCGTGCATCCGCAAAGCATCCTGC
TATGGGGGATTGTCGTCCAATTTGCCGCCAACCTGTCCCAACTCGCCGCCGTGCTGTTTTTCGGGTTGCCCCCGTTTTGG
CTGCTGGTTGCGTGCGTGATGTTTTCCGTCGGCACGCAGGGCTTGGTCGGCGCGAACACGCAGGCATGCTTTATGTCTTA
TTTCAAAGAAGAGGGCGGCAGCGCGAACGCCGTGTTGGGCGTGTTCCAGTCCTTAATCGGCGCGGGCGTGGGTATGGCGG
CGACCTTCTTGCACGACGGTTCGGCAACCGTGATGGCGGCAACGATGACCGCGTCCACCTCTTGCGGCATTGCGCTTCTG
TGGCTGTGTTCGCACCGGGCGTGGAAGGAAAACGAACAGAGCGAATACCTTTAA

Upstream 100 bases:

>100_bases
TCGTCCGGTTTTGCTGGGGCTTTTGTTGCCGCCTGTTTGTGCCGGTGTGTTAAAATTTTCCGTTTCCGCGTATTGTGTTT
TCCGCCGCCGGGCGGTTTGT

Downstream 100 bases:

>100_bases
CGGAAAATGCCGTCTGAAACCGTTTCAGACGGCATTTGATGTTAGAATGCACGATAAATTACTGTTCAGGCGAAATTATG
TCCCAAACTATCGACGAACT

Product: bicyclomycin resistance protein, putative

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MRIGREFMPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLNADVHRIEQSLSLFMFGTAFGQVVGGSVSD
IKGRKPVALTGLIVYCLAVAAIVFVSSAEQLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVA
PMVGALLQGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAAMGYLFFQAFSFGSMF
AFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRLKTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFW
LLVACVMFSVGTQGLVGANTQACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIALL
WLCSHRAWKENEQSEYL

Sequences:

>Translated_417_residues
MRIGREFMPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLNADVHRIEQSLSLFMFGTAFGQVVGGSVSD
IKGRKPVALTGLIVYCLAVAAIVFVSSAEQLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVA
PMVGALLQGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAAMGYLFFQAFSFGSMF
AFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRLKTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFW
LLVACVMFSVGTQGLVGANTQACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIALL
WLCSHRAWKENEQSEYL
>Mature_417_residues
MRIGREFMPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLNADVHRIEQSLSLFMFGTAFGQVVGGSVSD
IKGRKPVALTGLIVYCLAVAAIVFVSSAEQLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVA
PMVGALLQGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAAMGYLFFQAFSFGSMF
AFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRLKTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFW
LLVACVMFSVGTQGLVGANTQACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIALL
WLCSHRAWKENEQSEYL

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=354, Percent_Identity=30.225988700565, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI48994889, Length=235, Percent_Identity=34.8936170212766, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1790146, Length=344, Percent_Identity=27.6162790697674, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790794, Length=261, Percent_Identity=24.904214559387, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082312, Length=142, Percent_Identity=30.9859154929577, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787065, Length=250, Percent_Identity=24.8, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45228; Mature: 45228

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCHHHHHHHHCCCCCCEEEEEHHCCCCCHHHHHHHHHCHHCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHCCHHHHHHHHCCCCCCEEEEEHHCCCCCHHHHHHHHHCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]