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The map label for this gene is dnaG [H]

Identifier: 161870398

GI number: 161870398

Start: 1465610

End: 1467283

Strand: Direct

Name: dnaG [H]

Synonym: NMCC_1445

Alternate gene names: 161870398

Gene position: 1465610-1467283 (Clockwise)

Preceding gene: 161870397

Following gene: 161870399

Centisome position: 68.06

GC content: 53.46

Gene sequence:

>1674_bases
ATGGCGTGTTGCCCGTTCCACAAGGAAAAAACACCGTCGTTTTCGGTCAGTCCGACCAAGCAGTTTTACCACTGTTTCAG
TTGCGGGGCGCACGGTTCGGCGATTGGTTTTGTGATGGAGCATCAGGGGCTGTCGTTTCCGGAGGCGGTTCAGTTCCTTG
CCGACCGCGTGGGTATGATTGTGCCTAAAGTGCGCGGGCAGAACGATAATCCCGAAGTCCGTGCCGAACGTAAGAAAAAG
CAGCAGACGCTGGAAGAAACGACGGCGGCGGCGGCTGATTTTTATGCGCAACAGTTGCGCTTCAATCCGACGGCGAAGGC
TTATTTGGACAAGCGCGGCTTGAGCGCGGAAGTCATCGCGCATTATGGTTTGGGCTACGCGCCCGACGGCTGGCAGCCTT
TGGCGCAAGTATTCCAACCGTACCCGAATACCGCGTTGGTGGATACGGGTATGGTGATTGACAATGAGGGGCGGTATTAC
GACCGATTCCGTCATCGGATTATGTTCCCCATCCGCAATCCGCGCGGGCAGGTCATCGGTTTCGGCGGCAGGGTGCTGGA
CGACTCCAAACCCAAATATCTAAACTCTCCCGATACGCCTTTGTTTGATAAGGGGAAAAACCTTTACGGATTGTATGAAG
GGCGTGCTGCTGTCAAGGAAGTGGGGCGGATTCTGGTGGTCGAAGGCTATATGGACGTGGTCGCGCTGGCACAGTTCGGC
GTAGGCTACGGTGTGGCGGCCTTGGGCACGGCAACAACAGCGGAACACGTCAAAATCCTGATGCGTCAGGCGGACAGTAT
TTATTTCTGTTTCGACGGCGACAGCGCGGGGCGCAAAGCGGCTTGGCGCGCGCTGGAAAACGCGCTGCCGCAATTGAAGG
ACGACAAATCGCTGCATTTTTTGTTCCTGCCCGAGGAACACGACCCCGACAGCTACATCCGCGCCTACGGCAAAGCGCAA
TTTGAAGATGCGCTGTTGAATCAAAGCAAGCCCTTGTCGGAATATTTTTGGGAACATCTTACAGACGGCATTCATCTCAA
TACGCAGGAAGGCAAGGCGGAATTGGTAAAAACCAGTTCGCCGCTTTTGGCGCAGATTACCGCGCCGGCATTGGCTTATT
TGTTAAAACAACGGCTTAGCGAGCTGGTCGGCATCGACCCCGACAACCTCGCGCAACTGCTCGGACAGGAAGCGCCAAAG
CGGCACGTCAAACAAAAAAACTACAAACTGCCCCCGATTTCCGTCAAACAGCCCGTTATGCCGACATTGGTGCAACGGCA
AATCCGCAGCCTCTTGATAAATCCGGATTGGGCTGCATATATAGACCTGCCCGATTATTTGGCGTTGGACGGCGATTTCG
CCTGCCTTGCCAACCTCGCCGAAACCATTAAAAACCATCCTTCCGTGCCGGCAACCGCACAGGTTTTGGAACATATGCGC
GGTTCGCCCTACGAAGAAACAATCAACCGCATCTTCCGGTCGGCCCTTCAATCGGAAGAAATGGAAGGCGGCGGCGAAGA
AGATTGCGAAAACTTCCAAATCGGCATCAAAAAACTGCTCAATGAGTTAAAATACAGCCAAATCGAAACATTAAAACAAA
AAAGCCTGCAATCCGGCTTAAATGAAAGCGAGAAAAAACTTTTGCTGTCGCTGCTGACCGCAAAACAAAATTGA

Upstream 100 bases:

>100_bases
TATGATTCCATCCGACTTCATTGACGAACTTCTAGCCAAAACCGATATTGTCGACATCATCGACGAACAGGTTCCGCTGA
AAAAAGGCGGGGCGAACTAT

Downstream 100 bases:

>100_bases
CCGGCGGATTCCGCCATCCGTAAACCGTTATGCCGTCTGAAAAGCATTCACCCCGGCTGCAACAACGACACCTGCAGAAC
ACCCATCCCAAAAAGCCTTC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MACCPFHKEKTPSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQNDNPEVRAERKKK
QQTLEETTAAAADFYAQQLRFNPTAKAYLDKRGLSAEVIAHYGLGYAPDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYY
DRFRHRIMFPIRNPRGQVIGFGGRVLDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFG
VGYGVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFLFLPEEHDPDSYIRAYGKAQ
FEDALLNQSKPLSEYFWEHLTDGIHLNTQEGKAELVKTSSPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPK
RHVKQKNYKLPPISVKQPVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQVLEHMR
GSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQKSLQSGLNESEKKLLLSLLTAKQN

Sequences:

>Translated_557_residues
MACCPFHKEKTPSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQNDNPEVRAERKKK
QQTLEETTAAAADFYAQQLRFNPTAKAYLDKRGLSAEVIAHYGLGYAPDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYY
DRFRHRIMFPIRNPRGQVIGFGGRVLDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFG
VGYGVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFLFLPEEHDPDSYIRAYGKAQ
FEDALLNQSKPLSEYFWEHLTDGIHLNTQEGKAELVKTSSPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPK
RHVKQKNYKLPPISVKQPVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQVLEHMR
GSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQKSLQSGLNESEKKLLLSLLTAKQN
>Mature_556_residues
ACCPFHKEKTPSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQNDNPEVRAERKKKQ
QTLEETTAAAADFYAQQLRFNPTAKAYLDKRGLSAEVIAHYGLGYAPDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYD
RFRHRIMFPIRNPRGQVIGFGGRVLDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGV
GYGVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFLFLPEEHDPDSYIRAYGKAQF
EDALLNQSKPLSEYFWEHLTDGIHLNTQEGKAELVKTSSPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKR
HVKQKNYKLPPISVKQPVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQVLEHMRG
SPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQKSLQSGLNESEKKLLLSLLTAKQN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=499, Percent_Identity=43.8877755511022, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 62242; Mature: 62110

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACCPFHKEKTPSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMI
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
VPKVRGQNDNPEVRAERKKKQQTLEETTAAAADFYAQQLRFNPTAKAYLDKRGLSAEVIA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
HYGLGYAPDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIG
HCCCCCCCCCHHHHHHHHCCCCCCEEEECCCEEECCCCHHHHHHHHEECEEECCCCCEEE
FGGRVLDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFG
CCCEEECCCCCCCCCCCCCCHHHCCCCEEEEHHHHHHHHHCCCEEEEHHHHHHHHHHHHC
VGYGVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHF
CCCCHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEE
LFLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLTDGIHLNTQEGKAELVKTSS
EECCCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHCCC
PLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQPVM
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHH
PTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQVLEHMR
HHHHHHHHHHHCCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQKSLQSGL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NESEKKLLLSLLTAKQN
CHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ACCPFHKEKTPSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMI
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
VPKVRGQNDNPEVRAERKKKQQTLEETTAAAADFYAQQLRFNPTAKAYLDKRGLSAEVIA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
HYGLGYAPDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIG
HCCCCCCCCCHHHHHHHHCCCCCCEEEECCCEEECCCCHHHHHHHHEECEEECCCCCEEE
FGGRVLDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFG
CCCEEECCCCCCCCCCCCCCHHHCCCCEEEEHHHHHHHHHCCCEEEEHHHHHHHHHHHHC
VGYGVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHF
CCCCHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEE
LFLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLTDGIHLNTQEGKAELVKTSS
EECCCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCHHHHHCCC
PLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQPVM
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHH
PTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQVLEHMR
HHHHHHHHHHHCCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQKSLQSGL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NESEKKLLLSLLTAKQN
CHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]