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The map label for this gene is ackA1

Identifier: 161870377

GI number: 161870377

Start: 1445557

End: 1446753

Strand: Direct

Name: ackA1

Synonym: NMCC_1423

Alternate gene names: 161870377

Gene position: 1445557-1446753 (Clockwise)

Preceding gene: 161870369

Following gene: 161870378

Centisome position: 67.13

GC content: 58.06

Gene sequence:

>1197_bases
ATGTCCCAAAAATTGATCTTGGTTTTGAACTGCGGCAGCTCGTCCCTCAAAGGCGCGGTCCTGGATAACGGCAGCGGCGA
AGTCCTGCTCAGCTGCCTTGCCGAAAAACTCAACCTGCCCGATGCCTACATCACATTCAAAGTAAACGGCGAAAAACACA
AAGTCGATCTGTCCGCACATCCCGACCACACCGGCGCGGTCGAAGCCCTGATGGAAGAACTCAAAGCCCACGGCCTCGAC
AGCCGCATCGGCGCCATCGGCCACCGCGTCGTCAGCGGCGGCGAACTGTACAGCGAATCCATCCTCGTTGACGACGAAGT
CATTGCCGGCATCGAAAAATGCATCCCGCTCGCCCCCCTGCACAACCCCGCCCACCTCTTGGGCCTGCGTGCCGCGCAAA
GCATTTTCAAAGACCTGCCCAACGTCGTCGTATTCGATACCTCCTTCCACCAAACCATGCCCGAAGTCGCCTACAAATAC
GCCGTTCCGCAGGAGTTGTATGAAAAATACGGCCTGCGCCGTTACGGCGCGCACGGTACCAGCTACCGCTTCGTCGCCGA
CGAAACCGCGCGCTTCCTCGGCAAAGACAAAAAAGACCTGCGTATGGTCATTGCCCACTTGGGCAACGGCGCGTCCATCA
CCGCCGTCGCCAACGGCGAATCGCGCGACACCAGTATGGGCCTGACCCCGCTGGAAGGGCTGGTAATGGGTACGCGCAGC
GGCGACATCGATCCTTCCGTATTCGGCTTCCTTGCCGAAAACGCCAATATGACCATCGCCCAAATCACTGAAATGCTGAA
CAAAAAATCCGGTCTGCTCGGCATTTCCGGCCTGTCCAACGACTGCCGCACCATTGAAGAAGAAGCCGCCAAGGGGCATA
AAGGCGCGAAATTGGCCTTGGATATGTTTATCTACCGCCTTGCCAAATACATCGGCAGTATGGCGGTTGCCGCAGGCGGT
TTGGACGCACTGGTCTTTACCGGCGGCATCGGCGAAAACTCCGACATCATCCGCGAACGCGTGATCGGCTACTTGGGCTT
CCTCGGTCTGAACATCGACCAAGAAGCCAACCTGAAAGCCCGCTTCGGCAACGCCGGCGTGATTACCACTGCCGACAGCA
AAGCCGTTGCCGTGGTCATTCCGACCAACGAAGAGCTGATGATTGCCCACGACACTGCCCGTTTGAGCGGTCTGTAA

Upstream 100 bases:

>100_bases
CACTACTTTCCCGCCCAGGAAGGCAAAACGGATTCGGCACGAATCCGGTTAGTATCCGTGTCCGATTCCAATGCCGTCTG
AAACTTTCCGGAGTAAGAAA

Downstream 100 bases:

>100_bases
GGTTTTATCCGCACACGAACTGCCTCCGGAAATGGAGGCAGTTTTTTATCCGGCTTTCCATGCTTAAACAGCACTGCCTC
TTTTCAGACATTGACGGTTG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYITFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLD
SRIGAIGHRVVSGGELYSESILVDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVANGESRDTSMGLTPLEGLVMGTRS
GDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGISGLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGG
LDALVFTGGIGENSDIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDTARLSGL

Sequences:

>Translated_398_residues
MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYITFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLD
SRIGAIGHRVVSGGELYSESILVDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVANGESRDTSMGLTPLEGLVMGTRS
GDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGISGLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGG
LDALVFTGGIGENSDIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDTARLSGL
>Mature_397_residues
SQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYITFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDS
RIGAIGHRVVSGGELYSESILVDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKYA
VPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVANGESRDTSMGLTPLEGLVMGTRSG
DIDPSVFGFLAENANMTIAQITEMLNKKSGLLGISGLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGL
DALVFTGGIGENSDIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDTARLSGL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=401, Percent_Identity=51.1221945137157, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI145693184, Length=391, Percent_Identity=46.0358056265985, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA1_NEIMA (Q9JTM0)

Other databases:

- EMBL:   AL157959
- PIR:   A81868
- RefSeq:   YP_002343012.1
- ProteinModelPortal:   Q9JTM0
- SMR:   Q9JTM0
- EnsemblBacteria:   EBNEIT00000000706
- GeneID:   908107
- GenomeReviews:   AL157959_GR
- KEGG:   nma:NMA1718
- GeneTree:   EBGT00050000020921
- HOGENOM:   HBG285863
- ProtClustDB:   PRK00180
- BioCyc:   NMEN122587:NMA1718-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 42468; Mature: 42337

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYITFKVNGEKHKVDLSAH
CCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCC
PDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESILVDDEVIAGIEKCIPLAPL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHCCCEEECHHHHHHHHHHCCCCCC
HNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKYAVPQELYEKYGLRRYGAHGT
CCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCC
SYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVANGESRDTSMGLTPLEGLVMGTRS
EEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEEEECCC
GDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGISGLSNDCRTIEEEAAKGHKGAKLAL
CCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHH
DMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENSDIIRERVIGYLGFLGLNIDQEANLKA
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEE
RFGNAGVITTADSKAVAVVIPTNEELMIAHDTARLSGL
EECCCCEEEECCCCEEEEEEECCCCEEEEECCHHHCCC
>Mature Secondary Structure 
SQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYITFKVNGEKHKVDLSAH
CCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCC
PDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESILVDDEVIAGIEKCIPLAPL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHCCCEEECHHHHHHHHHHCCCCCC
HNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKYAVPQELYEKYGLRRYGAHGT
CCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCC
SYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVANGESRDTSMGLTPLEGLVMGTRS
EEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHEEEECCC
GDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGISGLSNDCRTIEEEAAKGHKGAKLAL
CCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHH
DMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENSDIIRERVIGYLGFLGLNIDQEANLKA
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEE
RFGNAGVITTADSKAVAVVIPTNEELMIAHDTARLSGL
EECCCCEEEECCCCEEEEEEECCCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919