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The map label for this gene is lysS [H]

Identifier: 161870293

GI number: 161870293

Start: 1344346

End: 1345821

Strand: Reverse

Name: lysS [H]

Synonym: NMCC_1335

Alternate gene names: 161870293

Gene position: 1345821-1344346 (Counterclockwise)

Preceding gene: 161870294

Following gene: 161870289

Centisome position: 62.5

GC content: 54.88

Gene sequence:

>1476_bases
TTGGACGAAAACCAAATCATCGCCCTGCGCCGCGAAAAACTGCACAACATCCGCCAACAGCGCAACGCCTATCCCAACGA
CTTCAAACGCGACAGCTTCGCCGCCGATTTGCACGCCCAATACGGCGAAATCGGCAAAGAAGAACTCGATCCGCAAGGCA
TTCCCGTCAAAGTGGCCGGCCGCATGATGCTGAAGCGTCAAATGGGCAAGGCGAGCTTTGCCACCATTCAAGACGTGTCC
GGGCAAATCCAGCTTTATCTGAACAACAAAGGCGTGAGCCAAGAAGTTTTGGACGACTTCAACCATTGGGATTTGGGCGA
CATCGTCGGCGCGGAAGGCACTTTGTTCAAAACCAACCACGGCGAACTGACCGTACGCGTGTCCGGCATCCGCCTGCTGT
CCAAATCCCTGCGCCCTCTGCCGGACAAACATAAAGGCTTGAGCGATCAGGAAACCAAATACCGTCAACGCTACGTCGAT
TTGATTGCCAACGAAGAATCGCGCAATACCTTCATCAAACGCAGCCAAATCATCCAATCCGTGCGTAATTTTATGGTGGG
CGAGCATTATCTCGAAGTCGAAACCCCGATGATGCACCCGATTCCCGGCGGCGCGACGGCAAAACCCTTCGTTACCCATC
ACAATGCCTTAGATATTCCGCTTTATTTGCGAATCGCCCCCGAGCTGTATCTGAAACGCCTGGTTGTCGGTGGTTTGGAA
CGCGTGTTTGAAATCAACCGCAGCTTCCGCAACGAAGGCATGTCCGTGCGCCACAACCCCGAATTCACCATGATCGAATT
CTACGAAGCCTTCTCCGACTACGAACGCATGATGCAGATGGCGGAAGACATCATCCGCAACGCATCGCACACGGTAAACG
GCACGGCAAACATCACTTACAACGGCAAAGAAGTCGATTTGGAAAGCCCGTTTGAACGCCTGACCATTCTCGAAGCCATC
AAAAAATACAATCCGCACTACACCGACGAGCAGTTGAACGATGCGGAATGGCTGAAAAAAGAAATCGTCAAACACGGCGA
AAGCCTGCCGCCGTCCCCGGGCATCGGCAGCCTGCAACTCGCGCTGTTTGAAGGTTGCGCCGAGGGCAAGCTGTGGAATC
CGACCTTCATCGTCGATTACCCGGTCGAAGTTTCCCCGTTGGCGCGCGCTTCGGATACCAAACAAGGTCTGACCGAACGT
TTCGAATTGTTCGTTGTCGGGCGCGAACTGGCAAACGGCTATTCCGAGTTGAACGACCCCGAAGACCAAGCCGAACGCTT
CAAAGCGCAAGTGGCGCAAAAAGACGCGGGCGACGACGAAGCGATGCACTACGATGCCGACTACATCCGCGCGATGGAGT
TCGGTCTGCCGCCGACCGGCGGTTGCGGTATCGGCATCGACCGCTTGGTAATGTTGCTGACCGATTCGCAAACCATCCGT
GACGTGATTCTGTTCCCGCAGATGCGCCCCGAGTAA

Upstream 100 bases:

>100_bases
ATTTTGTGATTCGGTCAGGTCGTCTGAAACAGATTGCGCCTGATTTATTTTTTCGGAAAACCCTATGAGCGAACAAAACC
ATCCGCAAACCGAGCCGCAG

Downstream 100 bases:

>100_bases
TCATAAAAACAGTTGAAATGGCAATGCCGTCTGAACCCGATTGGATTCAGACGGCATTTTGTATGGCAGGATTGATAGTG
AATTAACAAAAATCAGGACA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MDENQIIALRREKLHNIRQQRNAYPNDFKRDSFAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVS
GQIQLYLNNKGVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLSDQETKYRQRYVD
LIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGATAKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLE
RVFEINRSFRNEGMSVRHNPEFTMIEFYEAFSDYERMMQMAEDIIRNASHTVNGTANITYNGKEVDLESPFERLTILEAI
KKYNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDYPVEVSPLARASDTKQGLTER
FELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGDDEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIR
DVILFPQMRPE

Sequences:

>Translated_491_residues
MDENQIIALRREKLHNIRQQRNAYPNDFKRDSFAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVS
GQIQLYLNNKGVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLSDQETKYRQRYVD
LIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGATAKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLE
RVFEINRSFRNEGMSVRHNPEFTMIEFYEAFSDYERMMQMAEDIIRNASHTVNGTANITYNGKEVDLESPFERLTILEAI
KKYNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDYPVEVSPLARASDTKQGLTER
FELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGDDEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIR
DVILFPQMRPE
>Mature_491_residues
MDENQIIALRREKLHNIRQQRNAYPNDFKRDSFAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVS
GQIQLYLNNKGVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLSDQETKYRQRYVD
LIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGATAKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLE
RVFEINRSFRNEGMSVRHNPEFTMIEFYEAFSDYERMMQMAEDIIRNASHTVNGTANITYNGKEVDLESPFERLTILEAI
KKYNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDYPVEVSPLARASDTKQGLTER
FELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGDDEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIR
DVILFPQMRPE

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=511, Percent_Identity=42.4657534246575, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI194272210, Length=511, Percent_Identity=42.4657534246575, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI45439306, Length=472, Percent_Identity=23.728813559322, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI40789249, Length=295, Percent_Identity=24.406779661017, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790571, Length=492, Percent_Identity=56.7073170731707, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1789256, Length=491, Percent_Identity=57.4338085539715, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=30.4878048780488, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788173, Length=300, Percent_Identity=23, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=488, Percent_Identity=42.4180327868852, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535927, Length=488, Percent_Identity=42.4180327868852, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71994340, Length=488, Percent_Identity=42.4180327868852, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32566633, Length=307, Percent_Identity=25.0814332247557, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=514, Percent_Identity=43.3852140077821, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324256, Length=383, Percent_Identity=32.6370757180157, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321807, Length=461, Percent_Identity=26.8980477223427, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=478, Percent_Identity=23.6401673640167, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640849, Length=505, Percent_Identity=41.7821782178218, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=505, Percent_Identity=41.7821782178218, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24584738, Length=302, Percent_Identity=25.8278145695364, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136276, Length=481, Percent_Identity=22.6611226611227, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 55876; Mature: 55876

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDENQIIALRREKLHNIRQQRNAYPNDFKRDSFAADLHAQYGEIGKEELDPQGIPVKVAG
CCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCHHHHH
RMMLKRQMGKASFATIQDVSGQIQLYLNNKGVSQEVLDDFNHWDLGDIVGAEGTLFKTNH
HHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCCHHHCCCCCEEEEECC
GELTVRVSGIRLLSKSLRPLPDKHKGLSDQETKYRQRYVDLIANEESRNTFIKRSQIIQS
CEEEEEEHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VRNFMVGEHYLEVETPMMHPIPGGATAKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLE
HHHHHHCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECHHHHHHHHHHHHHH
RVFEINRSFRNEGMSVRHNPEFTMIEFYEAFSDYERMMQMAEDIIRNASHTVNGTANITY
HHHHHHHHHHHCCCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
NGKEVDLESPFERLTILEAIKKYNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQL
CCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHH
ALFEGCAEGKLWNPTFIVDYPVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDP
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEEEHHHHCHHHHCCCC
EDQAERFKAQVAQKDAGDDEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIR
HHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHH
DVILFPQMRPE
HHHHCCCCCCC
>Mature Secondary Structure
MDENQIIALRREKLHNIRQQRNAYPNDFKRDSFAADLHAQYGEIGKEELDPQGIPVKVAG
CCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCHHHHH
RMMLKRQMGKASFATIQDVSGQIQLYLNNKGVSQEVLDDFNHWDLGDIVGAEGTLFKTNH
HHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCCHHHCCCCCEEEEECC
GELTVRVSGIRLLSKSLRPLPDKHKGLSDQETKYRQRYVDLIANEESRNTFIKRSQIIQS
CEEEEEEHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VRNFMVGEHYLEVETPMMHPIPGGATAKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLE
HHHHHHCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECHHHHHHHHHHHHHH
RVFEINRSFRNEGMSVRHNPEFTMIEFYEAFSDYERMMQMAEDIIRNASHTVNGTANITY
HHHHHHHHHHHCCCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
NGKEVDLESPFERLTILEAIKKYNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQL
CCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHH
ALFEGCAEGKLWNPTFIVDYPVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDP
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEEEHHHHCHHHHCCCC
EDQAERFKAQVAQKDAGDDEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIR
HHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHH
DVILFPQMRPE
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA