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The map label for this gene is iscA [H]

Identifier: 161870255

GI number: 161870255

Start: 1297893

End: 1298273

Strand: Direct

Name: iscA [H]

Synonym: NMCC_1296

Alternate gene names: 161870255

Gene position: 1297893-1298273 (Clockwise)

Preceding gene: 161870254

Following gene: 161870256

Centisome position: 60.27

GC content: 51.18

Gene sequence:

>381_bases
ATGCCGTCCGAACCACCCGCCGCTTCAGGTCTGTCCGGGGCGATACAACAAGGAAGAAATATGATTACCCTTACCGAGAA
TGCCGCAAAACACATCAATGACTATCTCGCCAAACGCGGCAAAGGCTTGGGCGTACGCTTGGGTGTGAAAACCAGCGGCT
GCTCGGGGATGGCGTACAACCTTGAATTTGTCGATGAAGCCGATGGCGACGACCTGATTTTTGAAGGACACGGCGCACGC
ATTTATATCGACCCGAAAAGCCTGGTTTATCTGGACGGCACACAAGTCGATTACACCAAAGAAGGTTTGCAGGAAGGTTT
CAAATTTGAAAACCCCAATGTCAAAGACTCCTGCGGCTGCGGCGAAAGCTTCCATGTTTAA

Upstream 100 bases:

>100_bases
ACTGCTCCATCTTGGCTGAAGATGCGGTAAAAGCGGCCGTTGCCGACTACCGCAAACGTCAGGAAAACAGATAAAGCCCT
TCAGACGGCATCGTCCCGCA

Downstream 100 bases:

>100_bases
GACATAAAAATGGCGGGACAGTATCAAAACCGTCCCGCCATTTTTTCGCTTCCTGCCTGTTGTAGCTGCCTTTGCCTTTC
CTTTTCCGTTCCACCTTGTG

Product: HesB/YadR/YfhF family protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MPSEPPAASGLSGAIQQGRNMITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFVDEADGDDLIFEGHGAR
IYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGESFHV

Sequences:

>Translated_126_residues
MPSEPPAASGLSGAIQQGRNMITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFVDEADGDDLIFEGHGAR
IYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGESFHV
>Mature_125_residues
PSEPPAASGLSGAIQQGRNMITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFVDEADGDDLIFEGHGARI
YIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGESFHV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=113, Percent_Identity=38.9380530973451, Blast_Score=96, Evalue=6e-21,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=37.3493975903614, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788877, Length=105, Percent_Identity=66.6666666666667, Blast_Score=155, Evalue=8e-40,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=49.5238095238095, Blast_Score=115, Evalue=8e-28,
Organism=Escherichia coli, GI1786351, Length=108, Percent_Identity=39.8148148148148, Blast_Score=73, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25146411, Length=112, Percent_Identity=36.6071428571429, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193209025, Length=108, Percent_Identity=37.037037037037, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323001, Length=106, Percent_Identity=39.622641509434, Blast_Score=90, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24642263, Length=108, Percent_Identity=36.1111111111111, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 13531; Mature: 13400

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSEPPAASGLSGAIQQGRNMITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYN
CCCCCCCCCCCHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEE
LEFVDEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGC
EEEEECCCCCEEEEECCCCEEEECCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCC
GESFHV
CCCCCC
>Mature Secondary Structure 
PSEPPAASGLSGAIQQGRNMITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYN
CCCCCCCCCCHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEE
LEFVDEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGC
EEEEECCCCCEEEEECCCCEEEECCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCC
GESFHV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA