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The map label for this gene is nadE [H]

Identifier: 161870234

GI number: 161870234

Start: 1277306

End: 1278097

Strand: Direct

Name: nadE [H]

Synonym: NMCC_1275

Alternate gene names: 161870234

Gene position: 1277306-1278097 (Clockwise)

Preceding gene: 161870231

Following gene: 161870236

Centisome position: 59.32

GC content: 57.32

Gene sequence:

>792_bases
ATGGATACGCAGGCAGTTATCACACATATCGCCCGATGGTTGGACGAATACGCCACTCAGGCAAATGCAAAAGGATTCGT
CATAGGCGTTTCCGGCGGCATCGATTCCGCCGTCGTCTCCACACTCGCCGCCCGCACCGGCCGCCCCACGCTGCTTCTGG
ATATGCCGATACGCCAACACCCCGGCCAGCTTGAGCGGGCAAGGCTGCACATCCGCAATCTGCAACGGCAATATGCCAAT
GTAAGCGCGCAAACGGCCGATTTGACCGACACCTTCCAGACCTTTGAACAAACCGTCGGCATTCATCAGACGGCATTTGA
CAATCAGCCGCTTTCCCTCGCCAACGCCAAAAGCCGCCTGCGTATGCTGACCCTGTACTACTACGGGCAGATACACGGAC
TGCTGGTTACGGGGACAGGTAATAAGATTGAAGATTTCGGCGTGGGATTTTTTACCAAATACGGCGACGGCGGCGTAGAC
ATCAGCCCGATTGCCGACCTGACCAAAACGCAGGTTTACCGGCTTGCCGAAGCATTGGGCGTGGACGAGGCGATTCAAAA
AGCCCCGCCGACCGACGGCCTGTGGGATACGGAACGCACCGACGAAGAACAGATGGGCGCAAGCTATCCCGAACTGGAGT
GGGCAATGGGCGTGTACGGCACGCGCAAACCCGAAGATTTTGAAGGGCGGCAGCGCGAAGTTCTAGAAATCTATACGCGA
CTTCACCGCGCCATGCAGCACAAAATCAACCCGATTCCCGTATGCCGCATTCCGCCCGAATTGCTGGGCTGA

Upstream 100 bases:

>100_bases
TTAAGCGGGGAATGGGAAAAATATTCATCAACCTGCCTGCAATCTATTCAAATTGAACCGCCGACAAGCTATGATGCGGA
TATTTTCGACAGGAGGAACC

Downstream 100 bases:

>100_bases
AACACGGAAATGCCGTCTGAAACGGAAAACCGTATTTCAGACGGCATTGAAATATCCGACTCCTATCCCTTAAGAATCGA
GTACGCGGGCAAACAAAATA

Product: NH(3)-dependent NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MDTQAVITHIARWLDEYATQANAKGFVIGVSGGIDSAVVSTLAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYAN
VSAQTADLTDTFQTFEQTVGIHQTAFDNQPLSLANAKSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGFFTKYGDGGVD
ISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGASYPELEWAMGVYGTRKPEDFEGRQREVLEIYTR
LHRAMQHKINPIPVCRIPPELLG

Sequences:

>Translated_263_residues
MDTQAVITHIARWLDEYATQANAKGFVIGVSGGIDSAVVSTLAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYAN
VSAQTADLTDTFQTFEQTVGIHQTAFDNQPLSLANAKSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGFFTKYGDGGVD
ISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGASYPELEWAMGVYGTRKPEDFEGRQREVLEIYTR
LHRAMQHKINPIPVCRIPPELLG
>Mature_263_residues
MDTQAVITHIARWLDEYATQANAKGFVIGVSGGIDSAVVSTLAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYAN
VSAQTADLTDTFQTFEQTVGIHQTAFDNQPLSLANAKSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGFFTKYGDGGVD
ISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGASYPELEWAMGVYGTRKPEDFEGRQREVLEIYTR
LHRAMQHKINPIPVCRIPPELLG

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=207, Percent_Identity=30.9178743961353, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 29316; Mature: 29316

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQAVITHIARWLDEYATQANAKGFVIGVSGGIDSAVVSTLAARTGRPTLLLDMPIRQH
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHCC
PGQLERARLHIRNLQRQYANVSAQTADLTDTFQTFEQTVGIHQTAFDNQPLSLANAKSRL
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
RMLTLYYYGQIHGLLVTGTGNKIEDFGVGFFTKYGDGGVDISPIADLTKTQVYRLAEALG
HHHHHHEECEEEEEEEECCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
VDEAIQKAPPTDGLWDTERTDEEQMGASYPELEWAMGVYGTRKPEDFEGRQREVLEIYTR
HHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LHRAMQHKINPIPVCRIPPELLG
HHHHHHHCCCCCCEECCCHHHCC
>Mature Secondary Structure
MDTQAVITHIARWLDEYATQANAKGFVIGVSGGIDSAVVSTLAARTGRPTLLLDMPIRQH
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHCC
PGQLERARLHIRNLQRQYANVSAQTADLTDTFQTFEQTVGIHQTAFDNQPLSLANAKSRL
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
RMLTLYYYGQIHGLLVTGTGNKIEDFGVGFFTKYGDGGVDISPIADLTKTQVYRLAEALG
HHHHHHEECEEEEEEEECCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
VDEAIQKAPPTDGLWDTERTDEEQMGASYPELEWAMGVYGTRKPEDFEGRQREVLEIYTR
HHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LHRAMQHKINPIPVCRIPPELLG
HHHHHHHCCCCCCEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA