Click here to switch to the map view.

The map label for this gene is rluB [H]

Identifier: 161870231

GI number: 161870231

Start: 1271788

End: 1273038

Strand: Direct

Name: rluB [H]

Synonym: NMCC_1272

Alternate gene names: 161870231

Gene position: 1271788-1273038 (Clockwise)

Preceding gene: 161870229

Following gene: 161870234

Centisome position: 59.06

GC content: 57.71

Gene sequence:

>1251_bases
TTGAAAGATTCTACCCCCGCACACAAACCGATTTCAACCGTTGCACAAACTTTGTCCCCAAAGCCGCAGCGACGATTTCA
TCCGCAAAACCGCCGCATCAGGTACAATATCGAACCGTCCGACCGAGGGCGGCATTTTATCAACCCGTCCTGCCGCACAT
GCCGCAGAAGAACCGCTTTATCAAGCGAGTTAGGAAAAATGATGTCCAAGCAACCCACCAGCAAACGCCAATGGCGCGAC
GGCGCTGCCCCGTCTGCCAAGAAAACCGCCAAACCGTTCAAAAGCAAAGCCCGTCCCAAAGACGAAACACGCAAAACCGC
ATCCCAACCTTACGGACAAAAAGCTTCAGACGGCATCAAGCTGCAAAACACCCCCAAACAGCGTGCCGCCAAAGCCAAAA
AACTCGTCGTCCGCAATCCCAACCAAAAAATTATGGGACACGCGCGCGATTTGAAAGAACGCCGCAGCGACCTGTCGCGC
ATGGAACCCGAACGCCTGCAAAAAGTGCTTGCCGCGTCCGGCGTCGGCTCGCGCCGCGAAATGGAAGAATGGATTAACAA
CGGCTGGGTAACGGTCAACGGCAAAACCGCGCAACTGGGCGACAAAGTTACCCCCGACGACCACGTTACCGTCAAAGGCA
GTATCATCAAACTCAAATGGGCGGACCGCCTGCCGCGCATCATCCTGTATTACAAACAAGAAGGCGAAATCGTTTCCCGC
GACGATCCGCAAGGCCGCGTCAGCATATTCGACCGTCTGCCGCAGGCCGCCAGCAGCCGCTGGGTCGCCATCGGACGCTT
GGACATCAACACCAGCGGACTTCTGATTCTCACCACCTCCGGCGAACTCGTCCAACGTTTCGCCCACCCCAGCTTCGAAG
TCGAACGCGAATACGCCGTGCGCGTCTTGGGCGGGCTGACCGGCGAACAAATGCGCGTCCTCACCGAAGAAGGCGTGATG
CTCGAAGACGGCTTGGCAAAAGTCGAACGCATCCGCGAACAAGGCGGCGAAGGCGCGAACAAATGGTACAACGTCGTGAT
TAAAGAAGGCCGCAACCGCGAAGTGCGCCGCATTTTTGAAAGCCAAGGACTCACCGTCAGCCGCCTCGTGCGCGTCGGCT
TCGGCCCCATCGGACTGCCCAACCGCCTCAAACGCGGGCAGTTCTACGAACTCAACCCCGCCGAAGTCGCCAACATCATC
AAATGGGCGGACATGCTGCTGCCGGGCGAACGCCGCCGCAAAAAAGCCTAA

Upstream 100 bases:

>100_bases
TCTCTAAGGTGCTCAAGCACCAAGTGAATCGGTTCCGTACTATCTGTACTGTCTGCGGCTTCGTCGCCTTGTCCTGATTT
TTGTTAATCCACTATATCAT

Downstream 100 bases:

>100_bases
ACCCGCCAAAACACAAAAATGCCGTCTGAAACATCTACTGTTTCAGACGGCATTTTATTCGGGCGTTTTCAGGAGAAAAG
GTCGAGTGCTTTGACAAAGA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKDSTPAHKPISTVAQTLSPKPQRRFHPQNRRIRYNIEPSDRGRHFINPSCRTCRRRTALSSELGKMMSKQPTSKRQWRD
GAAPSAKKTAKPFKSKARPKDETRKTASQPYGQKASDGIKLQNTPKQRAAKAKKLVVRNPNQKIMGHARDLKERRSDLSR
MEPERLQKVLAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRIILYYKQEGEIVSR
DDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQRFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVM
LEDGLAKVERIREQGGEGANKWYNVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANII
KWADMLLPGERRRKKA

Sequences:

>Translated_416_residues
MKDSTPAHKPISTVAQTLSPKPQRRFHPQNRRIRYNIEPSDRGRHFINPSCRTCRRRTALSSELGKMMSKQPTSKRQWRD
GAAPSAKKTAKPFKSKARPKDETRKTASQPYGQKASDGIKLQNTPKQRAAKAKKLVVRNPNQKIMGHARDLKERRSDLSR
MEPERLQKVLAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRIILYYKQEGEIVSR
DDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQRFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVM
LEDGLAKVERIREQGGEGANKWYNVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANII
KWADMLLPGERRRKKA
>Mature_416_residues
MKDSTPAHKPISTVAQTLSPKPQRRFHPQNRRIRYNIEPSDRGRHFINPSCRTCRRRTALSSELGKMMSKQPTSKRQWRD
GAAPSAKKTAKPFKSKARPKDETRKTASQPYGQKASDGIKLQNTPKQRAAKAKKLVVRNPNQKIMGHARDLKERRSDLSR
MEPERLQKVLAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRIILYYKQEGEIVSR
DDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQRFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVM
LEDGLAKVERIREQGGEGANKWYNVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANII
KWADMLLPGERRRKKA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=235, Percent_Identity=53.1914893617021, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1790453, Length=240, Percent_Identity=30.8333333333333, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788510, Length=245, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=30.3867403314917, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 47207; Mature: 47207

Theoretical pI: Translated: 11.25; Mature: 11.25

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSTPAHKPISTVAQTLSPKPQRRFHPQNRRIRYNIEPSDRGRHFINPSCRTCRRRTAL
CCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
SSELGKMMSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTASQPYGQKASDGIK
HHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCE
LQNTPKQRAAKAKKLVVRNPNQKIMGHARDLKERRSDLSRMEPERLQKVLAASGVGSRRE
ECCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
MEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRIILYYKQEGEIVSR
HHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECEEEEEECHHCCCEEEEEECCCCCEECC
DDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQRFAHPSFEVEREYAV
CCCCCHHHHHHHCCHHHCCCEEEEEEEEECCCCEEEEECCHHHHHHHCCCCCHHHHHHHH
RVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWYNVVIKEGRNREVRRIFE
HHHHCCCCHHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCHHHHHHHH
SQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIKWADMLLPGERRRKKA
HCCCHHHHHHHHCCCCCCCCHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MKDSTPAHKPISTVAQTLSPKPQRRFHPQNRRIRYNIEPSDRGRHFINPSCRTCRRRTAL
CCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
SSELGKMMSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTASQPYGQKASDGIK
HHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCE
LQNTPKQRAAKAKKLVVRNPNQKIMGHARDLKERRSDLSRMEPERLQKVLAASGVGSRRE
ECCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
MEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRIILYYKQEGEIVSR
HHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECEEEEEECHHCCCEEEEEECCCCCEECC
DDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQRFAHPSFEVEREYAV
CCCCCHHHHHHHCCHHHCCCEEEEEEEEECCCCEEEEECCHHHHHHHCCCCCHHHHHHHH
RVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWYNVVIKEGRNREVRRIFE
HHHHCCCCHHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCHHHHHHHH
SQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIKWADMLLPGERRRKKA
HCCCHHHHHHHHCCCCCCCCHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]