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The map label for this gene is trmJ [H]

Identifier: 161870219

GI number: 161870219

Start: 1259349

End: 1260227

Strand: Direct

Name: trmJ [H]

Synonym: NMCC_1260

Alternate gene names: 161870219

Gene position: 1259349-1260227 (Clockwise)

Preceding gene: 161870216

Following gene: 161870220

Centisome position: 58.48

GC content: 55.52

Gene sequence:

>879_bases
TTGACCGTCCGAACGAAAAAGACGGCGCATTATACCCTATTCCATTCCGACCGAAAACCGAACATGACTACTCTCAAACC
CGCCCTGCCCGCTTATCTGGACAACATCCGCATCATCCTCACGCGCACCAGCCATCCCGCCAACATCGGCTCTGCCGCGC
GCGCGATGAAAACAATGGGTCTGCACAAACTGACCATCGTCGCCCCAAATCTGATGGCAACGCCGATGACGGAAAACCCG
CCCGTGTTTGACCCGGAGCATCCTCAATCGTTTAAATTACCGGAAGAAAGCTTCATCCTCGCTTCCGGCGCGGCAGACGT
TTTGGAAAATGCCACCATTGCCGCTTCTTTGGACGAAGCCCTTGCCGACACCACCATCGCCTGCGCCCTGACCAGCCGCC
GCCGCGAAATTACTGCGCCGCTGCAAACCCCGCGCGATTTGGTATCCGAATTACTGCAGACCGCAAACCGAGGCGAGAAA
GTGGCACTGGTTTTCGGCAACGAGACTTTCGGCTTGAGCATCGAAGAAGTCCAAGCCTGCAACCGACTGATGACCATCAA
CGGCAATCCCGACTATTTCTCGCTCAACCTCGCCCAAGCCGTGCAGGTCGTGTGCTACGAAATCTTCAGCCAAACCGGTT
CGCCCATGACCCATCTTCAACAAGAAGACCACGCTGCGACCCACGAGCAAATCAAAGGCATGGTCGCCCACATGGAAAGC
GTGATGAACGACATCGGCTTTTTCAACCGCCGCAATGGTGAACGCCTGATGCGCCGTATGCAGAGCCTGTTCGGACGCGC
CAACACGCAAACCGAAGACATCGATATCCTGCGCGGTTTTTTCAATACCGTCAGCCATCGTATCCATAAAAAAGACTGA

Upstream 100 bases:

>100_bases
GGTAAATCCTGCCAAATCGGGTAAAATACCGCCTGACGCGTGTCTGCTTCAGGCGCAACGTTAAATTTCCGACGTTGTTA
AAGAACATTTCAGACGGCAT

Downstream 100 bases:

>100_bases
TTAAGGCCGTCTGAAAACATTTCCAGCTTTTCAGACGGCATGACTGATATTCGGATAAGCATGAATTACGCCCTAGACGC
ATTATGGTGGAAGCTCACCA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTVRTKKTAHYTLFHSDRKPNMTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLTIVAPNLMATPMTENP
PVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALADTTIACALTSRRREITAPLQTPRDLVSELLQTANRGEK
VALVFGNETFGLSIEEVQACNRLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHMES
VMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD

Sequences:

>Translated_292_residues
MTVRTKKTAHYTLFHSDRKPNMTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLTIVAPNLMATPMTENP
PVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALADTTIACALTSRRREITAPLQTPRDLVSELLQTANRGEK
VALVFGNETFGLSIEEVQACNRLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHMES
VMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD
>Mature_291_residues
TVRTKKTAHYTLFHSDRKPNMTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLTIVAPNLMATPMTENPP
VFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALADTTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKV
ALVFGNETFGLSIEEVQACNRLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHMESV
MNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=260, Percent_Identity=35, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1790865, Length=199, Percent_Identity=32.6633165829146, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 32533; Mature: 32401

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRTKKTAHYTLFHSDRKPNMTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMG
CCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHC
LHKLTIVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEA
CEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHEEEECCHHHHHHCCHHHHHHHHH
LADTTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQAC
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHC
NRLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHMES
CEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVRTKKTAHYTLFHSDRKPNMTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMG
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHC
LHKLTIVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEA
CEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHEEEECCHHHHHHCCHHHHHHHHH
LADTTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQAC
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHC
NRLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHMES
CEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]