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The map label for this gene is proS [H]

Identifier: 161870212

GI number: 161870212

Start: 1245299

End: 1247098

Strand: Reverse

Name: proS [H]

Synonym: NMCC_1252

Alternate gene names: 161870212

Gene position: 1247098-1245299 (Counterclockwise)

Preceding gene: 161870217

Following gene: 161870211

Centisome position: 57.91

GC content: 54.78

Gene sequence:

>1800_bases
TTGGAGGTTGGTGCAAAACCTGCTTTTTTCGCGTTACAATCTGCACCCGATTTACTTTTGAATTTGCCTGTTTGGAAAAA
TACTTTTATGAAAGCCAGCCAATTCTTTATCTCTACTTTAAAAGAAGCCCCTGCCGAAGCCGCGCTTGCCAGCCACAAGC
TGATGATTCGCGCCGGTCTGATTAAAGCCAACGCGTCCGGCCTTTATACTTGGATGCCGATGGGGCTGCGCGTGTTACGC
AAAGTCGAAAACGTCGTGCGCGAGGAAATGGCGCGCGCGGGCAGCGTGGAGCTGCTGATGCCGGTGGTGCAGCCTGCCGA
GCTGTGGCAGGAATCCGGCCGCTGGGAATTTTACGGTAAAGAACTGCTGCGCCTGAAAGACCGCCACGACCGCGATTTCT
GCATGGGCCCGACCTGCGAGGAAGTCATCGCCGACATCGTGCGCAAAGAAATCAACAGCTACAAACAGCTGCCGAAAAAC
TTTTACCACATCCAAACCAAATTCCGCGACGAAGTGCGCCCGCGTTTCGGCGTGATGCGCGCGCGCGAGTTCGTCATGAA
AGACGCTTATTCCTTCCATGCCGACTACGCCTCGCTTCAGACGACCTATGATGCCATGTATGACTCTTACTGCCGCATCT
TCACCCGTCTGGGCTTGGCATTCCGCCCCGTCGCCGCAGACACCGGCAGCATCGGCGGTACGGGTTCGCACGAGTTTCAA
GTGTTGGCGGAAAGCGGCGAAGATGTTATTGCGTACAGCGACACTTCCGATTACGCCGCCAATATCGAGTTGGCACCGAC
CTTGCCGCTTAAAGGCGAACGCGCCGCTGCTCAGGCTGTGTTGACCAAAGTACATACACCAAACGTCAAAACCATTGAGT
CTTTGGTTGAATTCCTGAATATTCCGGTTGAACAAACCCTCAAATCCATCGTGGTCGAAGGCGAAAACGAAGGCGAAATC
GTCTTATTACTGTTGCGCGGCGACCATGAGTTCAACGACATCAAGGCAGAAAAACTCGCAGGCGTGAGATCGCCATTGAC
GATGGCAAGTCCTGCCGCGATTGTCGAACAGTTCGGCGCAAACGGCGGCTCGCTGGGTCCGGTCAGCTTTGCAGGTAAAG
TTTATGCCGATTTTGCTACTGAAAAAGGAGCGGACTGGGTCATCGGTGCGAACGAAGACGGCTACCACTACACCAGCTTC
AACTTCGGGCGCGATGCCGCCGAGCCTGAATTCGTCGATTTGCGTAATGTGGTCGAAGGCGACGAAAGCCCCGACGGGCA
AGGCCGTCTGAAACTCGCGCGCGGCATTGAAGTCGGACACGTCTTCCAGTTGCGCGACAAATATACTAAAGCCATGAACG
TCAGCTTCCTCGACAACAACGGCAAATCGCAAATTATGGAAATGGGCTGCTACGGCATCGGCATCACCCGCGTCGTTGCC
GCCGCCATCGAGCAGAATAACGACGAAAAAGGCATCATCTGGACCAAAGCAATGGCGCCGTTTGAAGTCGTTATCGTGCC
GATGAACTACAAAAAATCAGACACCGTACGCGAAGCCGCCGACAAAATCTATGCCGAATTGCTGGCGGCAGGCGCGGATG
TGCTGCTGGATGACCGCGACGAACGCGCGGGCGTATTGCTGAACGACTCCGAGCTGTTGGGCATCCCGCACCGCATCGTC
ATCGGCGACCGCGCCTTGAAAGAAGGCAATGTCGAATACGCCGAACGCCGCAACAACGAAGCGCAGGCAGTTGCAATCGG
AGAAATTGTTGCGCGTGTAACAGCTTCATTAAATGCGTAA

Upstream 100 bases:

>100_bases
ATCTATAAAAATCATGTGCTTATGTTCAATATGATGTTTCTGGTCATGTTTTTGCCGTCTGTTATGGGATTTCTCCCGCG
CTTGGGGCAGGGGGTGAATT

Downstream 100 bases:

>100_bases
GCTTGGTCAAATGCTGTCTGAAAATAATTTCAGACGGCATTTTTATTTGGGGAGAAAGTGAAGTGGCTTCAGTATTTTTA
GAAGGAGAAGCAGTCGAGGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MEVGAKPAFFALQSAPDLLLNLPVWKNTFMKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMPMGLRVLR
KVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDRDFCMGPTCEEVIADIVRKEINSYKQLPKN
FYHIQTKFRDEVRPRFGVMRAREFVMKDAYSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQ
VLAESGEDVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQTLKSIVVEGENEGEI
VLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGGSLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTSF
NFGRDAAEPEFVDLRNVVEGDESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVVA
AAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLDDRDERAGVLLNDSELLGIPHRIV
IGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTASLNA

Sequences:

>Translated_599_residues
MEVGAKPAFFALQSAPDLLLNLPVWKNTFMKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMPMGLRVLR
KVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDRDFCMGPTCEEVIADIVRKEINSYKQLPKN
FYHIQTKFRDEVRPRFGVMRAREFVMKDAYSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQ
VLAESGEDVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQTLKSIVVEGENEGEI
VLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGGSLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTSF
NFGRDAAEPEFVDLRNVVEGDESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVVA
AAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLDDRDERAGVLLNDSELLGIPHRIV
IGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTASLNA
>Mature_599_residues
MEVGAKPAFFALQSAPDLLLNLPVWKNTFMKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMPMGLRVLR
KVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDRDFCMGPTCEEVIADIVRKEINSYKQLPKN
FYHIQTKFRDEVRPRFGVMRAREFVMKDAYSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQ
VLAESGEDVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQTLKSIVVEGENEGEI
VLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGGSLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTSF
NFGRDAAEPEFVDLRNVVEGDESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVVA
AAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLDDRDERAGVLLNDSELLGIPHRIV
IGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTASLNA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=289, Percent_Identity=40.4844290657439, Blast_Score=218, Evalue=1e-56,
Organism=Escherichia coli, GI1786392, Length=564, Percent_Identity=60.8156028368794, Blast_Score=703, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=245, Percent_Identity=34.6938775510204, Blast_Score=158, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=32.9411764705882, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=537, Percent_Identity=29.9813780260708, Blast_Score=240, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24656200, Length=242, Percent_Identity=36.3636363636364, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 66180; Mature: 66180

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVGAKPAFFALQSAPDLLLNLPVWKNTFMKASQFFISTLKEAPAEAALASHKLMIRAGL
CCCCCCCHHEEECCCCHHEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECE
IKANASGLYTWMPMGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGK
EEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCHHHHHHHCCCEEEHHH
ELLRLKDRHDRDFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMR
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHH
AREFVMKDAYSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEE
VLAESGEDVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLN
EEECCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHC
IPVEQTLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGA
CCHHHHHHHHEECCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCC
NGGSLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTSFNFGRDAAEPEFVDLRNVVEG
CCCCCCCHHHCCHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHCC
DESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVVA
CCCCCCCCCEEEECCCCCCCEEHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCHHHHHH
AAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLDDRD
HHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCC
ERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTASLNA
CCCCEEECCCCEECCCCEEEECCHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEVGAKPAFFALQSAPDLLLNLPVWKNTFMKASQFFISTLKEAPAEAALASHKLMIRAGL
CCCCCCCHHEEECCCCHHEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECE
IKANASGLYTWMPMGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGK
EEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCHHHHHHHCCCEEEHHH
ELLRLKDRHDRDFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMR
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHH
AREFVMKDAYSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEE
VLAESGEDVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLN
EEECCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHC
IPVEQTLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGA
CCHHHHHHHHEECCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCC
NGGSLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTSFNFGRDAAEPEFVDLRNVVEG
CCCCCCCHHHCCHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHCC
DESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVVA
CCCCCCCCCEEEECCCCCCCEEHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCHHHHHH
AAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLDDRD
HHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCC
ERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTASLNA
CCCCEEECCCCEECCCCEEEECCHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA