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The map label for this gene is glyA [H]

Identifier: 161869938

GI number: 161869938

Start: 972618

End: 973868

Strand: Reverse

Name: glyA [H]

Synonym: NMCC_0971

Alternate gene names: 161869938

Gene position: 973868-972618 (Counterclockwise)

Preceding gene: 161869939

Following gene: 161869937

Centisome position: 45.22

GC content: 54.2

Gene sequence:

>1251_bases
ATGTTTTCAAAAAGCGTTACCCTCGCACAATACGACCCCGATTTGGCAGCAGCCATTGCCCAAGAAGACCAACGCCAGCA
AGACCACGTCGAGCTGATTGCTTCCGAAAACTACGTTAGCTGCGCCGTAATGGACGCGCAAGGTTCGCAATTGACCAACA
AATACGCCGAAGGCTATCCTGGCAAACGCTACTACGGCGGCTGCGAATACGTCGATATTGTCGAACAATTGGCGATTGAC
CGCGTCAAAAAACTGTTCGGCGCGCAATACGCCAACGTCCAACCGCACTCCGGCTCGCAAGCCAACCAAGCCGTGTACGC
TTCCGTTTTAAAACCAGGCGACACCATTTTGGGTATGTCTCTGGCGCACGGCGGCCACCTGACCCACGGCGCGAGCGTTA
ATATCTCAGGCAAACTCTACAATGCCGTTACTTATGGTTTGGATGAAAACGAAGTGCTCGATTATGCCGAAGTCGAACGC
CTCGCGCTCGAACACAAACCCAAAATGATTGTGGCGGGTGCGTCTGCCTACGCGTTGCAAATCGACTGGGCAAAATTCCG
CGAAATCGCCGATAAAGTCGGCGCATACCTCTTTGTCGATATGGCGCACTATGCCGGTCTGGTTGCCGGCGGCGAATATC
CCAATCCCGTGCCGTTCTGCGACTTCGTTACCACCACCACCCACAAAACCCTGCGCGGTCCTCGCGGCGGCGTAATTTTG
TGCCGCGACAACACACACGAAAAAGCACTGAACTCTTCCATCTTCCCAAGCCTGCAAGGCGGCCCGCTGATGCACGTTAT
CGCCGCCAAAGCCGTGGCGTTTAAAGAAGCATTGCAACCCGAGTTCAAACAATACGCAAAACAAGTGAAAATCAATGCAG
CCGCCATGGCGGAAGAGTTGGTTAAACGCGGTTTGCGCATCGTTTCCGGCCGCACCGAAAGCCACGTTTTCCTCGTTGAC
CTGCAACCGATGAAAATTACCGGCAAAGCCGCCGAAGCTGCTTTGGGCAAAGCGCACATCACTGTCAACAAAAACGCCAT
CCCGAATGATCCGGAAAAACCCTTCGTTACCTCCGGCATCCGCATCGGTTCCGCCGCCATGACCACACGCGGTTTTAACG
AAGCCGACGTGCGCGTATTGGCGAACTTGGTTGCCGACGTATTGTCTAACCCCGAAGACGAAGCCAACCTCGCCAAAGTC
CGCGAACAAGTAACTGCTTTGTGCAATAAATACCCCGTTTACGGCGCGTAA

Upstream 100 bases:

>100_bases
AAGAAAATTTACGCATTATGGGCTATCTACCTTAGGTATTGAAGTATTTTTTATACAAAAAACACTCATGATACCCTGAT
TACACAAAGGAGCCGAGAAC

Downstream 100 bases:

>100_bases
GCCTTTTTAAAAATATTCCGCCAAGCAATCCAATGCCGCCTGAAATCTCATAATGTTTCAGGCGGAAACCTTTGCAAAAT
CCCCCCAAATCCCCTAAATT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVMDAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAID
RVKKLFGAQYANVQPHSGSQANQAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVPFCDFVTTTTHKTLRGPRGGVIL
CRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKEALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVD
LQPMKITGKAAEAALGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADVRVLANLVADVLSNPEDEANLAKV
REQVTALCNKYPVYGA

Sequences:

>Translated_416_residues
MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVMDAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAID
RVKKLFGAQYANVQPHSGSQANQAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVPFCDFVTTTTHKTLRGPRGGVIL
CRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKEALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVD
LQPMKITGKAAEAALGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADVRVLANLVADVLSNPEDEANLAKV
REQVTALCNKYPVYGA
>Mature_416_residues
MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVMDAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAID
RVKKLFGAQYANVQPHSGSQANQAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVPFCDFVTTTTHKTLRGPRGGVIL
CRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKEALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVD
LQPMKITGKAAEAALGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADVRVLANLVADVLSNPEDEANLAKV
REQVTALCNKYPVYGA

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=454, Percent_Identity=41.1894273127753, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI261862352, Length=454, Percent_Identity=41.1894273127753, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI261862350, Length=454, Percent_Identity=41.1894273127753, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI261862348, Length=454, Percent_Identity=41.1894273127753, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI261862346, Length=453, Percent_Identity=41.280353200883, Blast_Score=324, Evalue=7e-89,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=41.3965087281795, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI22547189, Length=387, Percent_Identity=39.5348837209302, Blast_Score=251, Evalue=7e-67,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=64.6634615384615, Blast_Score=547, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI25144732, Length=396, Percent_Identity=43.9393939393939, Blast_Score=330, Evalue=9e-91,
Organism=Caenorhabditis elegans, GI25144729, Length=396, Percent_Identity=43.9393939393939, Blast_Score=330, Evalue=9e-91,
Organism=Saccharomyces cerevisiae, GI6319739, Length=438, Percent_Identity=40.8675799086758, Blast_Score=324, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6323087, Length=429, Percent_Identity=41.4918414918415, Blast_Score=319, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24640005, Length=454, Percent_Identity=41.6299559471366, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI221329721, Length=454, Percent_Identity=41.6299559471366, Blast_Score=347, Evalue=9e-96,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45002; Mature: 45002

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVMDAQGSQLTNKYAEGYP
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEECCCCHHHHHHHCCCC
GKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQANQAVYASVLKPGDTILGMS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
LAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVERLALEHKPKMIVAGASAYALQ
ECCCCCCCCCCEEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEE
IDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVPFCDFVTTTTHKTLRGPRGGVIL
ECHHHHHHHHHHHHHEEEEEHHHHHHEEECCCCCCCCCCHHHHHHCCHHHHCCCCCCEEE
CRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKEALQPEFKQYAKQVKINAAAMAEEL
EECCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAALGKAHITVNKNAIPNDPEKPFVTSGI
HHCCHHEECCCCCCEEEEEEECCCEEECHHHHHHCCEEEEEECCCCCCCCCCCCEEECCE
RIGSAAMTTRGFNEADVRVLANLVADVLSNPEDEANLAKVREQVTALCNKYPVYGA
EECCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVMDAQGSQLTNKYAEGYP
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEECCCCHHHHHHHCCCC
GKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQANQAVYASVLKPGDTILGMS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
LAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVERLALEHKPKMIVAGASAYALQ
ECCCCCCCCCCEEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEE
IDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVPFCDFVTTTTHKTLRGPRGGVIL
ECHHHHHHHHHHHHHEEEEEHHHHHHEEECCCCCCCCCCHHHHHHCCHHHHCCCCCCEEE
CRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKEALQPEFKQYAKQVKINAAAMAEEL
EECCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAALGKAHITVNKNAIPNDPEKPFVTSGI
HHCCHHEECCCCCCEEEEEEECCCEEECHHHHHHCCEEEEEECCCCCCCCCCCCEEECCE
RIGSAAMTTRGFNEADVRVLANLVADVLSNPEDEANLAKVREQVTALCNKYPVYGA
EECCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10417653 [H]