Click here to switch to the map view.

The map label for this gene is rluF [H]

Identifier: 161869750

GI number: 161869750

Start: 775268

End: 776026

Strand: Reverse

Name: rluF [H]

Synonym: NMCC_0770

Alternate gene names: 161869750

Gene position: 776026-775268 (Counterclockwise)

Preceding gene: 161869751

Following gene: 161869747

Centisome position: 36.04

GC content: 54.41

Gene sequence:

>759_bases
ATGAATTCCAAAATTTCCTCCGACCATACAGAAGATGCCGTGCGCCTCTCCAAACGCATGGCGCAACTGGGGCTTTGTTC
ACGCCGCGAAGCCGACGGCTACATCGGACAGGGCTGGGTAACGGTCAACGGCAAAACCGCCGTACTCGGTCAGAAAGTTT
CACCGGCAGACCGTATCGAACTGAACAAGAAAGCCCACGAACAGCAGGCGGCACGCATTACCATCCTGCTGAACAAACCT
GTCGGCTATGTCAGCGCACAGGCGGAAAAAGGCTATAAATCCGCCGCCGAACTGATTACCTCTGAAAATCGCTGGGAAGG
TGACACCAGCCGCATCCGTTTCGATCCGAAACACAAAATCGGTCTTGCCCCCGCCGGCAGGCTCGATATCGATTCAGTCG
GATTACTGGTGTTGACGCAGGACGGCCGTATCGCCAAGCAGCTTATCGGCGAAAACAGCGGCAGTGAAAAAGAATATTTG
GTGCGCGTGCGCGGCAAATTGGACGAAAAAGGACTTGCCTTACTGAATCACGGATTGAGTTTGGACGGTGAGAAACTGCG
TCCCGCCAAAGTAGAATGGCAAAACGAAGACCAACTGCGTTTCGTATTGAAACAAGGTAAAAAGCGGCAAATCCGCCGTA
TGTGCGAACTGGTCGGACTGCGCGTCGTCGGGCTGAAACGCATCCGCATGGGTAAGGTCAAGCTCGGCAGGCTGCCGCCC
GGCAAATGGCGTTATCTTGCTCCCGGCGAATCGTTTTAA

Upstream 100 bases:

>100_bases
CATCCTACATCCGACCGACTACCAATATTTCAAAACCCTGCGCCAAAAACTGCACTGGGGCGAACAATTAGTCTAAGCCG
GCCTTTACCCGAAAGTATCC

Downstream 100 bases:

>100_bases
ATAAGCATTAAAAAATGCCGTCTGAACGTCCTTACGTTTTCAGACGGCATTGCCTTATGCCCAAATAACGGTTTCTTCCA
AGGGCCTGCGCGCTTTCGTG

Product: RNA-binding protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNSKISSDHTEDAVRLSKRMAQLGLCSRREADGYIGQGWVTVNGKTAVLGQKVSPADRIELNKKAHEQQAARITILLNKP
VGYVSAQAEKGYKSAAELITSENRWEGDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
VRVRGKLDEKGLALLNHGLSLDGEKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELVGLRVVGLKRIRMGKVKLGRLPP
GKWRYLAPGESF

Sequences:

>Translated_252_residues
MNSKISSDHTEDAVRLSKRMAQLGLCSRREADGYIGQGWVTVNGKTAVLGQKVSPADRIELNKKAHEQQAARITILLNKP
VGYVSAQAEKGYKSAAELITSENRWEGDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
VRVRGKLDEKGLALLNHGLSLDGEKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELVGLRVVGLKRIRMGKVKLGRLPP
GKWRYLAPGESF
>Mature_252_residues
MNSKISSDHTEDAVRLSKRMAQLGLCSRREADGYIGQGWVTVNGKTAVLGQKVSPADRIELNKKAHEQQAARITILLNKP
VGYVSAQAEKGYKSAAELITSENRWEGDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
VRVRGKLDEKGLALLNHGLSLDGEKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELVGLRVVGLKRIRMGKVKLGRLPP
GKWRYLAPGESF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=35.9504132231405, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788510, Length=241, Percent_Identity=29.4605809128631, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=31.9838056680162, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081838, Length=220, Percent_Identity=29.0909090909091, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28100; Mature: 28100

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKISSDHTEDAVRLSKRMAQLGLCSRREADGYIGQGWVTVNGKTAVLGQKVSPADRIE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCEEEECCCEEEECCCCCCHHHHH
LNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSENRWEGDTSRIRFDPKHKI
CCHHHHHHCCEEEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCC
GLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYLVRVRGKLDEKGLALLNHGLS
CCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCCCC
LDGEKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELVGLRVVGLKRIRMGKVKLGRLPP
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHCCHHHEEECCEEECCCCC
GKWRYLAPGESF
CCEEEECCCCCC
>Mature Secondary Structure
MNSKISSDHTEDAVRLSKRMAQLGLCSRREADGYIGQGWVTVNGKTAVLGQKVSPADRIE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCEEEECCCEEEECCCCCCHHHHH
LNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSENRWEGDTSRIRFDPKHKI
CCHHHHHHCCEEEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCC
GLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYLVRVRGKLDEKGLALLNHGLS
CCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCCCC
LDGEKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELVGLRVVGLKRIRMGKVKLGRLPP
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHCCHHHEEECCEEECCCCC
GKWRYLAPGESF
CCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]