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The map label for this gene is ngoFVIIM [H]

Identifier: 161869727

GI number: 161869727

Start: 750782

End: 751906

Strand: Reverse

Name: ngoFVIIM [H]

Synonym: NMCC_0747

Alternate gene names: 161869727

Gene position: 751906-750782 (Counterclockwise)

Preceding gene: 161869729

Following gene: 161869726

Centisome position: 34.92

GC content: 41.69

Gene sequence:

>1125_bases
ATGCTATCTAAACAAATCTCAAATCTTAATTCTTCTAGCAATAAACCAAAAATCCTATCTCTATTTTCAGGATGTGGCGG
TTTGGATTTGGGCTTTCATCAAGCTGGTTATGAAACTGTTTGGGCGAACGATTTCTCCCATTGGGCTTGCGAAAGTTTCC
GTAAAAATATCGGCGATGTCATCGTAGAAGGTGATATTGAACAAATTGATCCGAATAATCCAACTATTCCCGATTGCGAC
ATCATTTTAGGCGGATTCCCTTGTCAAGATTTTTCCATGATTTGGAAACAGCCGGGCTTAGAGGGTGAGCGCGGCAATCT
TTATAAAAGCTTTTTACGTTTTGTAAATGCAAAAAAACCGAAAGTTTTTGTTGCTGAGAATGTGAAAGGTTTATTGACTG
CTAACAAGAAAAAAGCCATCCAGCAAATTATTACCGACTTTGAAAATTGCGGTTATTACGTTCAGGCGAAGCTGTATAAC
TTTGCTGAATTTGGCGTACCTCAATTTCGTGAACGTGTGCTAATTGTCGGTGTGCGTTTGGATACGGGTTTCGACTTCCA
CCACCCTGAGCCTACTCACGGTGAAAATACGGATTACGGTCTTTTACCTTATGTAACCGCTGGTCAGGCTATTTCTGATA
TTCCTGAAAACGCAAACAATCACGAGCAGTTAAAAATCAGTGAACAAACTAAAAAACGGTTGAATCTGATTCCAGAAGGA
GGAAATTACAGCGACATTCCTAGAGATCACCCTTTATACGTTAAAAGTATGTTGAGTTTGGTTTACCGACGAATGCATCG
GGACAAACCTTCTACAACCATCATTGCTGCTGGCGGTGGAGGTACTTGGGGTTATCACTTCCCTGAACCGCGTGCCTTTA
CCAACCGCGAACGGGCAAGATTACAGAGTTTTCCCGATAATTTTGAGTTTATGGGCTCAACCACGGAAGTCCGCCGTCAA
ATCGGAAACGCCGTTCCTCCACAGGGTGTAGTAGAACTAGCAAAAGCCATACTTCCAGTTTTTTCAGACGGTTATCACAA
GGTAAATCTACATGAAAAATTAGAAGCAGAAAAAAACGTCTTCTTCCAAGACAGACTGAACAAAATCCGTGGGGGAAAAC
GATGA

Upstream 100 bases:

>100_bases
TGCGTTGCGTTGCGTTGCGTTGCGTTGCGTTCAGAGCATAGTTCACCTTTAAAATCCATACAAGGATTTTTATCAAATTT
ATGCATAGGAGTGAAATCCT

Downstream 100 bases:

>100_bases
ATACGGTTTTTTCCAACATTGCCAATGCCAAAATCACAGATAAATCCTTGAATAAGGTTTGGATGGATTTGTTTATGTCT
GCCGATGATATGTTGATGGC

Product: site-specific DNA-methyltransferase M.NgoVII

Products: NA

Alternate protein names: M.NgoFVII; Cytosine-specific methyltransferase NgoFVII; M.NgoVII [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGYETVWANDFSHWACESFRKNIGDVIVEGDIEQIDPNNPTIPDCD
IILGGFPCQDFSMIWKQPGLEGERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
FAEFGVPQFRERVLIVGVRLDTGFDFHHPEPTHGENTDYGLLPYVTAGQAISDIPENANNHEQLKISEQTKKRLNLIPEG
GNYSDIPRDHPLYVKSMLSLVYRRMHRDKPSTTIIAAGGGGTWGYHFPEPRAFTNRERARLQSFPDNFEFMGSTTEVRRQ
IGNAVPPQGVVELAKAILPVFSDGYHKVNLHEKLEAEKNVFFQDRLNKIRGGKR

Sequences:

>Translated_374_residues
MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGYETVWANDFSHWACESFRKNIGDVIVEGDIEQIDPNNPTIPDCD
IILGGFPCQDFSMIWKQPGLEGERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
FAEFGVPQFRERVLIVGVRLDTGFDFHHPEPTHGENTDYGLLPYVTAGQAISDIPENANNHEQLKISEQTKKRLNLIPEG
GNYSDIPRDHPLYVKSMLSLVYRRMHRDKPSTTIIAAGGGGTWGYHFPEPRAFTNRERARLQSFPDNFEFMGSTTEVRRQ
IGNAVPPQGVVELAKAILPVFSDGYHKVNLHEKLEAEKNVFFQDRLNKIRGGKR
>Mature_374_residues
MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGYETVWANDFSHWACESFRKNIGDVIVEGDIEQIDPNNPTIPDCD
IILGGFPCQDFSMIWKQPGLEGERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
FAEFGVPQFRERVLIVGVRLDTGFDFHHPEPTHGENTDYGLLPYVTAGQAISDIPENANNHEQLKISEQTKKRLNLIPEG
GNYSDIPRDHPLYVKSMLSLVYRRMHRDKPSTTIIAAGGGGTWGYHFPEPRAFTNRERARLQSFPDNFEFMGSTTEVRRQ
IGNAVPPQGVVELAKAILPVFSDGYHKVNLHEKLEAEKNVFFQDRLNKIRGGKR

Specific function: This methylase recognizes the double-stranded sequence GCSGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoFVII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=292, Percent_Identity=26.3698630136986, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4503351, Length=292, Percent_Identity=26.3698630136986, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788271, Length=365, Percent_Identity=25.4794520547945, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 42180; Mature: 42180

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGYETVWANDFSHWACESFRKNIGDV
CCCHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCEEECCCCCHHHHHHHHHHHCCE
IVEGDIEQIDPNNPTIPDCDIILGGFPCQDFSMIWKQPGLEGERGNLYKSFLRFVNAKKP
EEECCHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
KVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYNFAEFGVPQFRERVLIVGVRL
EEEEECCCCHHHCCHHHHHHHHHHHHHHHCCEEEEEEEHHHHHCCCHHHCCCEEEEEEEE
DTGFDFHHPEPTHGENTDYGLLPYVTAGQAISDIPENANNHEQLKISEQTKKRLNLIPEG
ECCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHCCCCCCCCCEEEEHHHHHHHHCCCCCC
GNYSDIPRDHPLYVKSMLSLVYRRMHRDKPSTTIIAAGGGGTWGYHFPEPRAFTNRERAR
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHH
LQSFPDNFEFMGSTTEVRRQIGNAVPPQGVVELAKAILPVFSDGYHKVNLHEKLEAEKNV
HHCCCCCHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCH
FFQDRLNKIRGGKR
HHHHHHHHHCCCCC
>Mature Secondary Structure
MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGYETVWANDFSHWACESFRKNIGDV
CCCHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCEEECCCCCHHHHHHHHHHHCCE
IVEGDIEQIDPNNPTIPDCDIILGGFPCQDFSMIWKQPGLEGERGNLYKSFLRFVNAKKP
EEECCHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
KVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYNFAEFGVPQFRERVLIVGVRL
EEEEECCCCHHHCCHHHHHHHHHHHHHHHCCEEEEEEEHHHHHCCCHHHCCCEEEEEEEE
DTGFDFHHPEPTHGENTDYGLLPYVTAGQAISDIPENANNHEQLKISEQTKKRLNLIPEG
ECCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHCCCCCCCCCEEEEHHHHHHHHCCCCCC
GNYSDIPRDHPLYVKSMLSLVYRRMHRDKPSTTIIAAGGGGTWGYHFPEPRAFTNRERAR
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHH
LQSFPDNFEFMGSTTEVRRQIGNAVPPQGVVELAKAILPVFSDGYHKVNLHEKLEAEKNV
HHCCCCCHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCH
FFQDRLNKIRGGKR
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]