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The map label for this gene is ngoFVIIR [H]

Identifier: 161869726

GI number: 161869726

Start: 749744

End: 750538

Strand: Reverse

Name: ngoFVIIR [H]

Synonym: NMCC_0746

Alternate gene names: 161869726

Gene position: 750538-749744 (Counterclockwise)

Preceding gene: 161869727

Following gene: 161869725

Centisome position: 34.85

GC content: 36.6

Gene sequence:

>795_bases
TTGAACGACTTTTTGCAAGATGAACAGCGTGGAATGGTTTATGTGTCGCCGTGGGTAAAGTTTCACGGGAAAATGTATTC
ATTTCAAAATCAGCGGGAAATCCATACACTAATCGGTTCGGCAAATCTGACTTGTTTTTGGGATAAAGCCGAACGAACTT
ATGAAACGATGGTGCATTTGGATGATTATCAAATAGCGTCTAAATTGCGGGAAGATATTGCTCAAACTATTAAGCAGCTT
GGAATTCCTATCAACAAAGCAGATAAGCCAGTAGAGTTTAAAGAACACAATGTTCATTTAGAGCAATGTTTAGGTGTTGA
GAAAATAAGTAAAGAAAGAGTAGAAAAACTATTTACTCAAACTTCTCAATATTATTTTGAATTACCTGTTAAAACTGAAC
CTAGAAGCAGTTTAAATCGTTTTTTTGCGGAAGGTAGAAAAAATCAACGGGGCTTTTATTTACCTCGACCTTGGTATGAG
GCAGAGTTGATTGTTTCAACAAGATTTTTAACTGCAGAGGGTTATCCAAAGGAAAAAAACTTTACAGTTATTACAGATGA
TGGCTGGCAGTTTGAATGTAATACGACAGGACAAAATGGTAAAAATTTCAGTTCAAAAGGTGACCAAACTATTTTTGGAA
AATGGGTAAAAGGACGTTTGGAAGCAACCGGCTGTTTAAAAACAGGTGAACCTGTAACAGAAGAAACCTTACGCCAATAC
GGTAACGATCATTTTGAGTTCCGTTCAACCGACAATCCTGATGTTTGGCTGCTCTCATTTAAGGGGAAAAATTAA

Upstream 100 bases:

>100_bases
ATTTTTGGAAGAAAGCGATCATAAACAAAAACTCAGTTTGATGGTCGGAATGCACTATTTAGAAGGTTTCAGTCGCCCAC
AATACAGCAGCTTATGCGAA

Downstream 100 bases:

>100_bases
ATGTTGCGAACCTACTTGAATCAATTAACCCCTCCCGAATTAGCTGACTCTGTTGAAAATACTGTTAATGGTTTTATGGA
GAAATTATCACAAACCGAGC

Product: restriction endonuclease R.NgoVII

Products: NA

Alternate protein names: R.NgoFVII; Endonuclease NgoFVII; R.NgoVII; Type II restriction enzyme NgoFVII [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNDFLQDEQRGMVYVSPWVKFHGKMYSFQNQREIHTLIGSANLTCFWDKAERTYETMVHLDDYQIASKLREDIAQTIKQL
GIPINKADKPVEFKEHNVHLEQCLGVEKISKERVEKLFTQTSQYYFELPVKTEPRSSLNRFFAEGRKNQRGFYLPRPWYE
AELIVSTRFLTAEGYPKEKNFTVITDDGWQFECNTTGQNGKNFSSKGDQTIFGKWVKGRLEATGCLKTGEPVTEETLRQY
GNDHFEFRSTDNPDVWLLSFKGKN

Sequences:

>Translated_264_residues
MNDFLQDEQRGMVYVSPWVKFHGKMYSFQNQREIHTLIGSANLTCFWDKAERTYETMVHLDDYQIASKLREDIAQTIKQL
GIPINKADKPVEFKEHNVHLEQCLGVEKISKERVEKLFTQTSQYYFELPVKTEPRSSLNRFFAEGRKNQRGFYLPRPWYE
AELIVSTRFLTAEGYPKEKNFTVITDDGWQFECNTTGQNGKNFSSKGDQTIFGKWVKGRLEATGCLKTGEPVTEETLRQY
GNDHFEFRSTDNPDVWLLSFKGKN
>Mature_264_residues
MNDFLQDEQRGMVYVSPWVKFHGKMYSFQNQREIHTLIGSANLTCFWDKAERTYETMVHLDDYQIASKLREDIAQTIKQL
GIPINKADKPVEFKEHNVHLEQCLGVEKISKERVEKLFTQTSQYYFELPVKTEPRSSLNRFFAEGRKNQRGFYLPRPWYE
AELIVSTRFLTAEGYPKEKNFTVITDDGWQFECNTTGQNGKNFSSKGDQTIFGKWVKGRLEATGCLKTGEPVTEETLRQY
GNDHFEFRSTDNPDVWLLSFKGKN

Specific function: Recognizes the double-stranded sequence GCSGC and cleaves after G-4 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019065 [H]

Pfam domain/function: PF09565 RE_NgoFVII [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 30872; Mature: 30872

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFLQDEQRGMVYVSPWVKFHGKMYSFQNQREIHTLIGSANLTCFWDKAERTYETMVHL
CCCCHHHHHCCEEEEEHHHHHCCHHCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHCC
DDYQIASKLREDIAQTIKQLGIPINKADKPVEFKEHNVHLEQCLGVEKISKERVEKLFTQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHHHHHHHH
TSQYYFELPVKTEPRSSLNRFFAEGRKNQRGFYLPRPWYEAELIVSTRFLTAEGYPKEKN
CCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCC
FTVITDDGWQFECNTTGQNGKNFSSKGDQTIFGKWVKGRLEATGCLKTGEPVTEETLRQY
EEEEECCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHCEECCCCCCCCCCCCHHHHHHHH
GNDHFEFRSTDNPDVWLLSFKGKN
CCCCEEECCCCCCCEEEEEECCCC
>Mature Secondary Structure
MNDFLQDEQRGMVYVSPWVKFHGKMYSFQNQREIHTLIGSANLTCFWDKAERTYETMVHL
CCCCHHHHHCCEEEEEHHHHHCCHHCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHCC
DDYQIASKLREDIAQTIKQLGIPINKADKPVEFKEHNVHLEQCLGVEKISKERVEKLFTQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHHHHHHHH
TSQYYFELPVKTEPRSSLNRFFAEGRKNQRGFYLPRPWYEAELIVSTRFLTAEGYPKEKN
CCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCC
FTVITDDGWQFECNTTGQNGKNFSSKGDQTIFGKWVKGRLEATGCLKTGEPVTEETLRQY
EEEEECCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHCEECCCCCCCCCCCCHHHHHHHH
GNDHFEFRSTDNPDVWLLSFKGKN
CCCCEEECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]