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The map label for this gene is rfbD [H]

Identifier: 161869700

GI number: 161869700

Start: 723976

End: 724851

Strand: Reverse

Name: rfbD [H]

Synonym: NMCC_0720

Alternate gene names: 161869700

Gene position: 724851-723976 (Counterclockwise)

Preceding gene: 161869703

Following gene: 161869699

Centisome position: 33.66

GC content: 55.37

Gene sequence:

>876_bases
ATGAGAATCCTATTGACAGGCTCGAAAAGCCAACTGGCACGCTGCCTGCGCGACCGTCTTCCGGAAGACTGGGAAACCAT
TGCGACGGATTCCGCATCCCTAGACATTACCGATGCCGATGCCGTCTGCAACATGGTCAAAAGTTTCCAACCCGACGCCA
TTGTCAACACGGCTGCCTATACTGCCGTCGACAAAGCGGAAGGCGATGCGGCAGCGGCATTTGCCGTCAATGCTTCCGCC
GTTTACAACCTTGCCTTGGCAGCACATCGCGCCCATGCCCGATTCATCCACATCTCAACCGACTATATCTTTGACGGAAA
AGGGAAAAGACCCTACCAGGAAAGCGACTTTACCAACCCTTCCAATGTATACGGACAATCCAAAACCGCCGGCGAACTGC
TCGCACTGTCTGCCAATCCCGACAGCCTTATCCTGCGGACTTCTTGGCTGTTTAGCGAATACGGGGACAACTTTGTCCGC
ACCATGCTGAACCTTGCGCGGGAACGTTCCCCGCTGTCCGCCGTCCACAACCAAATCGGCTGCCCGACCTATGCCGGCGA
CTTGTCCGCCGCCATCATCCGCCTGTTGCAGCAGTCCAATCCCGTTCGCGGCATTTACCACTACGTCGGCGGCAAATCCG
TATCCTGGTACGAATTTGCCCAACATATTTTCCAAGCGGCATCGCAACAGCAGACATCCTTCCCCGTTCCCGAATTGACT
GCCGTTTCAGACAAGGAATATCCGACCGCCGCCCCCAGGCCCGCATACAGCATTTTGGACTGCCGCAAAATCGAAAACGA
CTTCAGCATCAAACCGTCAGACTGGCAAAAAGCCCTTGCACAGGTCGTTTCCAAGCTGCTCTCTAGTGGGTACTAG

Upstream 100 bases:

>100_bases
CCATGGCACAAAAAGCCAACCCGTCAACCGTCGGCAAAGTTTTGGCACTATAATACCGACAGCAAGTCCTACAATACACT
TTTACCAAAGGAAATACCTC

Downstream 100 bases:

>100_bases
GTATAATGCAGGCATCCGATAAAGCAAAGCCCCCGAAGCTGCAACTTCGGGGGCTTGTCGGATAGAAAGGAGGCTTGAAG
AAATGGCGAAAGTCATTTTA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCNMVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASA
VYNLALAAHRAHARFIHISTDYIFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYEFAQHIFQAASQQQTSFPVPELT
AVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQKALAQVVSKLLSSGY

Sequences:

>Translated_291_residues
MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCNMVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASA
VYNLALAAHRAHARFIHISTDYIFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYEFAQHIFQAASQQQTSFPVPELT
AVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQKALAQVVSKLLSSGY
>Mature_291_residues
MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCNMVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASA
VYNLALAAHRAHARFIHISTDYIFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYEFAQHIFQAASQQQTSFPVPELT
AVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQKALAQVVSKLLSSGY

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=279, Percent_Identity=29.0322580645161, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI11034825, Length=279, Percent_Identity=29.0322580645161, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1788352, Length=296, Percent_Identity=38.1756756756757, Blast_Score=201, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31924; Mature: 31924

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCNMVKSFQPDAIVNTAAY
CEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHH
TAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDYIFDGKGKRPYQESDFTNP
HHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHEEEEEEEECEEECCCCCCCCCCCCCCCC
SNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVRTMLNLARERSPLSAVHNQIG
HHHCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHCC
CPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYEFAQHIFQAASQQQTSFPVPELT
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
AVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQKALAQVVSKLLSSGY
ECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCNMVKSFQPDAIVNTAAY
CEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHH
TAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDYIFDGKGKRPYQESDFTNP
HHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHEEEEEEEECEEECCCCCCCCCCCCCCCC
SNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVRTMLNLARERSPLSAVHNQIG
HHHCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHCC
CPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYEFAQHIFQAASQQQTSFPVPELT
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
AVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQKALAQVVSKLLSSGY
ECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]