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The map label for this gene is pcm [H]

Identifier: 161869330

GI number: 161869330

Start: 337365

End: 338021

Strand: Direct

Name: pcm [H]

Synonym: NMCC_0336

Alternate gene names: 161869330

Gene position: 337365-338021 (Clockwise)

Preceding gene: 161869329

Following gene: 161869331

Centisome position: 15.67

GC content: 53.27

Gene sequence:

>657_bases
ATGGATTTTGAAAAAGCGCGGTTCAATATGGTCGAACAGCAAATCCGTCCTTGGGATGTATTGGATTTTGACGTTTTGGA
CGCTTTGGCGGAGATTCCGCGCGAGCTTTTTGTCGATGAGGACTTGCAGGGTTTGGCGTATGCGGATATGGCGCTGCCGC
TTGCCAACGGTCATAAGATGCTCGAGCCGAAAGTCGTGGCGCGGCTGGCGCAGGGCTTGAAGCTGACGAAAAACGATACG
GTTTTGGAAATCGGCACGGGTTCGGGCTATGCGACCGCGCTGTTGGCAAAATTGGCTGGTCGTGTGGTGTCGGACGACAT
CGATGCCGAACAGCAAAACCGCGCCAAAGCGGTGTTGGACGGCTTGGGTTTGGACAATATCGATTATGTGCAAAACAACG
GGTTGACCGAACTTTCCGCAGGTGCGCCGTTTGATGCGGTTTATGTCGGCGGTGCGGTAAACCTTGTGCCTGAAGTGTTG
AAAGAACAGTTGAAAGATGGCGGACGCATGGCGGTTATCGTAGGCCGCAAGCCTGTTCAGCGCGCGCTTTTGATTACGCG
CAAGGGCGATGTGTTTGAAGAGAAGGTGCTGTTCGATACCTTGGTGGCGCATTTGGATGATAAGGATGCCGATCCGTTTG
GCGGTTTTCAGTTTTGA

Upstream 100 bases:

>100_bases
TGAGGGAAAGGGGCGGGCGGCGCGTGCCGAAGTTGGCGCGGCGGGAGTGTGGTTTTATAATGCCTGCCATTGTTGAAACA
ATTATTTGACGGAGCACTAA

Downstream 100 bases:

>100_bases
TGTTCGGATTGCGATGCCGTCTGAAAGCGGGTTTGGGGCTTCAGACGGCATTTTGCTTGGTTTTTTGGGATTTTTGCGAT
GGATATTGTGCAACTTGGGC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2 [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQGLAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDT
VLEIGTGSGYATALLAKLAGRVVSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF

Sequences:

>Translated_218_residues
MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQGLAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDT
VLEIGTGSGYATALLAKLAGRVVSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
>Mature_218_residues
MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQGLAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDT
VLEIGTGSGYATALLAKLAGRVVSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=190, Percent_Identity=38.9473684210526, Blast_Score=117, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23797; Mature: 23797

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQGLAYADMALPLANGHKM
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
LEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRVVSDDIDAEQQNRAKAVLD
CCHHHHHHHHHCCEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVLKEQLKDGGRMAVIVGRKPVQ
HCCCCCCHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCHH
RALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
HHHEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQGLAYADMALPLANGHKM
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
LEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRVVSDDIDAEQQNRAKAVLD
CCHHHHHHHHHCCEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVLKEQLKDGGRMAVIVGRKPVQ
HCCCCCCHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCHH
RALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
HHHEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA