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The map label for this gene is lgtE [H]

Identifier: 161869287

GI number: 161869287

Start: 298798

End: 299619

Strand: Direct

Name: lgtE [H]

Synonym: NMCC_0292

Alternate gene names: 161869287

Gene position: 298798-299619 (Clockwise)

Preceding gene: 161869286

Following gene: 161869289

Centisome position: 13.88

GC content: 47.08

Gene sequence:

>822_bases
TTGGCTTCTGCCAAAGAGCGCAGGGCGCACATTGCCGATACCTTCGGCAGGCACGACATTCCGTTTCAGTTTTTCGACGC
ACTGATGCCGTCTGAAGAACTGAATCGGATGATGGCGGAATTGGTGCCGGGATTGGCAAAACAGCACCTGTTGAGCGAAG
TGGAAAAAGCCTGCTTTATGAGCCACGCCGTATTGTGGAAACAGGCATTGGATGAAGGTCTGCCCTATGTTGCCGTATTT
GAGGATGATGTCCTGCTTGGCAAAGACGCAGAAAAGTTCCTTGCCGAAGATACTTGGTTGGAAGATCGTTTTGATAAGGA
TTCTGCCTTTATCGTCCGTTTGGAAACGATGTTTGCGAAAGTTATTGTCAGACCGGATAAAGTCCTGAATTATGAGAACC
GGTCATTTCCTTTGCTGGAGAGCGAACATTGGGGGACGGCTGGCTATATCATTTCGCGTGAGGCGATGCGGTTTTTCTTG
GAAAGGTTTGCCGTTTTGCCGGCAGAGTGGATTAAAGCGGTAGATTGGATGATGTTTACTTATTTCTTTGATAAGGAGGG
GATGCCTGTTTATCAGGTTAATCCCGCCTTATGTACCCAAGAATTGCATTATGCCAAGTTCCTCAGTAAAAACAGTATGT
TGGGTAGCGATTTGGAAAAAGATAGGGAACAAGGAAGAAGACACCGCCGTTCGTTGAAGGTGATGTTTGACTTGAAGCGT
GCTTTGGGTAAATTCGGTAGAGAAAAGAAGAAAAGAATGGAGCGTCAAAGGCAGGCGGAGCTTGAGAAAGCTTACGGCAG
GCGGGTCATATCGTTCAAATAG

Upstream 100 bases:

>100_bases
ACTTCGGCATTTTGCACCGATTGCTGAAAAACCGTTGAAAAACGCCGCTTTATCCAACAGACAAAAAACAGGATAAATTA
TGCAAAACCACGTTATCAGC

Downstream 100 bases:

>100_bases
TTTGTGTAAAATATAGCGGATTAAAATTAGAAATGGATACACTGTCATTCCCGCGCAGGCGGGAATCTAGTCCGTTCGGT
TTCGCTTGTTTTAAGTTTCG

Product: lacto-N-neotetraose biosynthesis glycosyl transferase LgtE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MASAKERRAHIADTFGRHDIPFQFFDALMPSEELNRMMAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVF
EDDVLLGKDAEKFLAEDTWLEDRFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISREAMRFFL
ERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKNSMLGSDLEKDREQGRRHRRSLKVMFDLKR
ALGKFGREKKKRMERQRQAELEKAYGRRVISFK

Sequences:

>Translated_273_residues
MASAKERRAHIADTFGRHDIPFQFFDALMPSEELNRMMAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVF
EDDVLLGKDAEKFLAEDTWLEDRFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISREAMRFFL
ERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKNSMLGSDLEKDREQGRRHRRSLKVMFDLKR
ALGKFGREKKKRMERQRQAELEKAYGRRVISFK
>Mature_272_residues
ASAKERRAHIADTFGRHDIPFQFFDALMPSEELNRMMAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFE
DDVLLGKDAEKFLAEDTWLEDRFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISREAMRFFLE
RFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKNSMLGSDLEKDREQGRRHRRSLKVMFDLKRA
LGKFGREKKKRMERQRQAELEKAYGRRVISFK

Specific function: Adds the first galactose to the lacto-N-tetraose chain in lipooligosaccharide (LOS) [H]

COG id: COG3306

COG function: function code M; Glycosyltransferase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 25 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002654 [H]

Pfam domain/function: PF01755 Glyco_transf_25 [H]

EC number: NA

Molecular weight: Translated: 32110; Mature: 31978

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAKERRAHIADTFGRHDIPFQFFDALMPSEELNRMMAELVPGLAKQHLLSEVEKACFM
CCCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLAEDTWLEDRFDKDSAFIVRLETMFAK
HHHHHHHHHHHCCCCEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VIVRPDKVLNYENRSFPLLESEHWGTAGYIISREAMRFFLERFAVLPAEWIKAVDWMMFT
HHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
YFFDKEGMPVYQVNPALCTQELHYAKFLSKNSMLGSDLEKDREQGRRHRRSLKVMFDLKR
HHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALGKFGREKKKRMERQRQAELEKAYGRRVISFK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ASAKERRAHIADTFGRHDIPFQFFDALMPSEELNRMMAELVPGLAKQHLLSEVEKACFM
CCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLAEDTWLEDRFDKDSAFIVRLETMFAK
HHHHHHHHHHHCCCCEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VIVRPDKVLNYENRSFPLLESEHWGTAGYIISREAMRFFLERFAVLPAEWIKAVDWMMFT
HHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
YFFDKEGMPVYQVNPALCTQELHYAKFLSKNSMLGSDLEKDREQGRRHRRSLKVMFDLKR
HHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALGKFGREKKKRMERQRQAELEKAYGRRVISFK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7964493 [H]