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The map label for this gene is lgtB [H]

Identifier: 161869285

GI number: 161869285

Start: 297933

End: 298628

Strand: Direct

Name: lgtB [H]

Synonym: NMCC_0290

Alternate gene names: 161869285

Gene position: 297933-298628 (Clockwise)

Preceding gene: 161869284

Following gene: 161869286

Centisome position: 13.84

GC content: 56.47

Gene sequence:

>696_bases
ATGGCGGAACTCGTCCCCGGTTTGTCGGCGCACCCCTATTTGAGCGGAGTGGAAAAAGCCTGCTTTATGAGCCACGCCGT
ATTGTGGAAGCAGGCATTGGACGAAGGTCTGCCGTATATCGCCGTATTTGAGGACGACGTTTTACTCGGCGAAAGCGCGG
AGAAATTCCTTGCCGAAGACGCTTGGCTGCAAGAACGCTTTGACCCCGATTCCGCCTTTATCGTCCGCTTGGAAACCATG
TTTATGCACGTCCTGACCTCGCCCTCCGGCGTGGCGGACTACTGCGGGCGTGCCTTTCCGCTGTTGGAAAGCGAACACTG
GGGGACGGCGGGCTATATCATTTCCAAAAAAGCGATGCGGTTTTTCCTGGACAGGTTTGCCGCCCTGCCGCCCGAAGGGC
TGCACCCCGTCGATCTGATGATGTTCAGCGATTTTTTCGACAGGGAAGGAGTGCCGGTTTGCCAGCTCAATCCCGCCTTG
TGCGCCCAAGAGCTGCATTATGCCAAGTTTCACGACCAAAACAGCGCATTGGGCAGCCTGATCGAACACGACCGCCTCCT
GAACCGCAAACAGCAATGGCGCGATTCCCCCGCCAACACATTCAAACGCCGCCTGATCCGCGCCTTGACCAAAATCAGCA
GGGAAAGGGAAAAACGCCGGCAAAGGCGCGAACAGTTCATTGTGCCTTTCCAATAA

Upstream 100 bases:

>100_bases
CGCAGAACGCAGGGCGCACATTGCCGCAACCTTCGGCGCGCGCGGCATCCCGTTTCAGTTTTTCGACGCACTGATGCCGT
CTGAAAGGCTGGAGCAGGCA

Downstream 100 bases:

>100_bases
AAGGAGAAAAGATGGACATCGTATTTGCGGCAGACGGCAGGATGCGGCGGCTGTTTACCTTGAGGCAATACTTCGGCATT
TTGCACCGATTGCTGAAAAA

Product: lacto-N-neotetraose biosynthesis glycosyl transferase LgtB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGESAEKFLAEDAWLQERFDPDSAFIVRLETM
FMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISKKAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPAL
CAQELHYAKFHDQNSALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ

Sequences:

>Translated_231_residues
MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGESAEKFLAEDAWLQERFDPDSAFIVRLETM
FMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISKKAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPAL
CAQELHYAKFHDQNSALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ
>Mature_230_residues
AELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGESAEKFLAEDAWLQERFDPDSAFIVRLETMF
MHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISKKAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALC
AQELHYAKFHDQNSALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ

Specific function: Adds the second galactose to the lacto-N-tetraose chain in lipooligosaccharide (LOS) [H]

COG id: COG3306

COG function: function code M; Glycosyltransferase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 25 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002654 [H]

Pfam domain/function: PF01755 Glyco_transf_25 [H]

EC number: NA

Molecular weight: Translated: 26604; Mature: 26473

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGESAEKFLAED
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHH
AWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISKKAMR
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQNSALGSL
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCCHHHHHH
IEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGESAEKFLAED
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHH
AWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISKKAMR
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQNSALGSL
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCCHHHHHH
IEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7964493 [H]