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The map label for this gene is glyS [H]

Identifier: 161869283

GI number: 161869283

Start: 294633

End: 296696

Strand: Direct

Name: glyS [H]

Synonym: NMCC_0288

Alternate gene names: 161869283

Gene position: 294633-296696 (Clockwise)

Preceding gene: 161869282

Following gene: 161869284

Centisome position: 13.68

GC content: 56.15

Gene sequence:

>2064_bases
ATGATGACCCAAACCCTTTTAATCGAACTCCTTACCGAAGAACTCCCGCCCAAAGCTCTGAATAATCTGGGCAACCATTT
TGCCGCCGCCGTTGCCGAAGGCTTGGAAAAAGCGCAACTGGTTGACGGTGCAGCCGAATTTACGGCTTATGCCTCGCCGC
GCCGTTTGGCGGTTCAAGTCAAAAACGTTAAAGCCGTTCAGGCCGATCAGAAAATCGTGAAAAAAGGCCCTGCCGTGGCG
AATGCCATGAAAGACGGTGCGCCGACCAAGGCTTTGGAAGGTTTCGCACGCGGTGCGGGCGCGAAAATCGAAGACTTGAC
CATCGTCCACGACGGCAAGCAGGACGTGTACGCCTACGAATACGTCCAAACCGGCAAACCGTTGGGCGAGCTTTTGGAAG
ACATTATCAATGCCGCAGTGAAAAAACTGCCGATTCCGAAAGTCATGCGTTGGGGCAGCAGCACGTTTACCTTTGTGCGC
CCCGTTCACGGGCTAGTTGTACTGCACGGCGGCGACATCGTAAACGTCAGCGTTTTGGGTCTGCAAAGCAGCAATCAAAC
CTTGGGACACCGCTTCTTGTCCAACGGCGAAATCACCATTGAAAACGCCGACAGCTACGCCGCACAAATGCGCGAGCAAG
GCAAAGTCGTCGCTTCGTTTGCCGAGCGCAAAGCTGCGATTCAGACGGCATTGGAAGGGCAGGCACGCCGTCTGAACGCC
TCCGTTGCCGCCGATGAAGCATTGTTGGACGAAGTAACCGCGCTGGTCGAATGGCCTGTGGTATTGGAAGCCGGTTTTGA
GGAACATTTCCTCGCCGTGCCGCAGGAATGCTTGATTTTAACGATGCAGCAAAACCAAAAATACTTTCCGCTGCTCGACC
AAAACGGCAAGCTGATGAACCGCTTCCTGCTGGTTTCCAACCTGCAAACCGAAGACCCGTCGCACATCATCCGAGGCAAC
GAACGCGTTTTGCGCGCGCGCCTGTCTGATGCCGAGTTCTTCTACAAACAAGACCAAAAAGCGACTTTGGAAAGCCGCCT
GCCGAAATTGTCCAGCGTGGTGTACCACAACAAAATCGGCTCGCAAGCCGAACGCATCGAACGCCTGCAAAGCATTGCCG
CCCACATCGCCAAAGCATTGGGCGCGGATGCCGCCGCTGCCGAACGCGCCGCGCGTCTGGCAAAAGCCGACTTGGTAACC
GAAATGGTCGGCGAGTTCCCTGAATTGCAAGGCACCATGGGCAAATACTATGCCCGTTTGGACGGCGAAACCGAAGAAAT
TGCCGAAGCCGTCGAGCAACATTACCAACCGCGTTTTGCCGGCGACAACTTGCCGAACGGCAAAGTCGCCACTGCCGTTG
CACTTGCCGACAAGCTGGAAACCCTGGTCGGCATTTGGGGCATCGGTCTGATTCCTACCGGCGATAAAGACCCCTACGCC
CTGCGCCGCGCCGCCTTGGGTATTTTGCGGATGCTGATGCAGTACGGTTTGGACGTGAACGAACTGATTCAGACGGCATT
CGACAGCTTCCCCAAAGGTTTGCTCAACGAGAAAACGCCGTCTGAAACCGCCGACTTTATGCAGGCGCGCCTTGCCGTGT
TGCTGCAAAACGATTATCCGCAAGACATCGTTGCCGCCGTATTGGCGAAACAGCCGCGCCGTTTGGACGACGTAGTGGCG
AAACTGCAAGCCGTTGCCGCGTTCAAACAACTGCCCGAAGCCGCCGCGCTCGCCGCCGCCAACAAACGCGTGCAAAATCT
GCTGAAAAAAGCCGATGCCGCGTTGGGCGAAGTCAATGAAAGCCTGTTGCAACAGGACGAAGAAAAAGCCCTCTTTGCCG
CCGCGCAAGGCTTGCAGCCGAAAATCGCCGCCGCCGTCGCCGAAGGCAATTTCCAAACCGCCTTGTCCGAACTGGCTTCC
GTCAAACCGCAAGTCGATGCATTCTTTGACGGCGTGATGGTAATGGCGGAAGATGCCGCCGTAAAACAAAACCGCCTGAA
CCTGCTGAACCGCTTGGCAGAGCAAATGAACGCGGTAGCCGACATCGCGCTTTTGGGCGAGTAA

Upstream 100 bases:

>100_bases
ACGCCACCCCGCTGGTTGACGGTTTTTACGGGGATGACGGCTGGGAAAATTACAGCGAAACCTATAAACAGGCAGATTAA
CCAAATTCAAAGAAAGCCGA

Downstream 100 bases:

>100_bases
CCGTTGTACAGTCCAAATGCCGTCTGAAGCCTTCAGACGGCATCGTGCCTATCGGGAGAATAAATTGCAGCCTTTAGTCA
GCGTATTGATTTGCGCCTAC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGAAEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVA
NAMKDGAPTKALEGFARGAGAKIEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
PVHGLVVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAAQMREQGKVVASFAERKAAIQTALEGQARRLNA
SVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQECLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGN
ERVLRARLSDAEFFYKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAARLAKADLVT
EMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDNLPNGKVATAVALADKLETLVGIWGIGLIPTGDKDPYA
LRRAALGILRMLMQYGLDVNELIQTAFDSFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVA
KLQAVAAFKQLPEAAALAAANKRVQNLLKKADAALGEVNESLLQQDEEKALFAAAQGLQPKIAAAVAEGNFQTALSELAS
VKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADIALLGE

Sequences:

>Translated_687_residues
MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGAAEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVA
NAMKDGAPTKALEGFARGAGAKIEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
PVHGLVVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAAQMREQGKVVASFAERKAAIQTALEGQARRLNA
SVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQECLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGN
ERVLRARLSDAEFFYKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAARLAKADLVT
EMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDNLPNGKVATAVALADKLETLVGIWGIGLIPTGDKDPYA
LRRAALGILRMLMQYGLDVNELIQTAFDSFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVA
KLQAVAAFKQLPEAAALAAANKRVQNLLKKADAALGEVNESLLQQDEEKALFAAAQGLQPKIAAAVAEGNFQTALSELAS
VKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADIALLGE
>Mature_687_residues
MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGAAEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVA
NAMKDGAPTKALEGFARGAGAKIEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
PVHGLVVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAAQMREQGKVVASFAERKAAIQTALEGQARRLNA
SVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQECLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGN
ERVLRARLSDAEFFYKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAARLAKADLVT
EMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDNLPNGKVATAVALADKLETLVGIWGIGLIPTGDKDPYA
LRRAALGILRMLMQYGLDVNELIQTAFDSFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVA
KLQAVAAFKQLPEAAALAAANKRVQNLLKKADAALGEVNESLLQQDEEKALFAAAQGLQPKIAAAVAEGNFQTALSELAS
VKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADIALLGE

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=690, Percent_Identity=45.9420289855072, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 74519; Mature: 74519

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGAAEFTAYASPRRLAVQV
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEE
KNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAKIEDLTIVHDGKQDVYAYE
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHH
YVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVRPVHGLVVLHGGDIVNVSVLG
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHEECCCCEEEEECCCEEEEEEEE
LQSSNQTLGHRFLSNGEITIENADSYAAQMREQGKVVASFAERKAAIQTALEGQARRLNA
ECCCCCHHHHHHHCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQECLILTMQQNQKYFPLLDQNGKLMN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHEEEEECCCCCCCCEECCCCHHHH
RFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFFYKQDQKATLESRLPKLSSVVYHNKIG
HHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCC
SQAERIERLQSIAAHIAKALGADAAAAERAARLAKADLVTEMVGEFPELQGTMGKYYARL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
DGETEEIAEAVEQHYQPRFAGDNLPNGKVATAVALADKLETLVGIWGIGLIPTGDKDPYA
CCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LRRAALGILRMLMQYGLDVNELIQTAFDSFPKGLLNEKTPSETADFMQARLAVLLQNDYP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
QDIVAAVLAKQPRRLDDVVAKLQAVAAFKQLPEAAALAAANKRVQNLLKKADAALGEVNE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLQQDEEKALFAAAQGLQPKIAAAVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAA
HHHHCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCHHHEEHHHH
VKQNRLNLLNRLAEQMNAVADIALLGE
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGAAEFTAYASPRRLAVQV
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEE
KNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAKIEDLTIVHDGKQDVYAYE
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHH
YVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVRPVHGLVVLHGGDIVNVSVLG
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHEECCCCEEEEECCCEEEEEEEE
LQSSNQTLGHRFLSNGEITIENADSYAAQMREQGKVVASFAERKAAIQTALEGQARRLNA
ECCCCCHHHHHHHCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQECLILTMQQNQKYFPLLDQNGKLMN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHEEEEECCCCCCCCEECCCCHHHH
RFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFFYKQDQKATLESRLPKLSSVVYHNKIG
HHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCC
SQAERIERLQSIAAHIAKALGADAAAAERAARLAKADLVTEMVGEFPELQGTMGKYYARL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
DGETEEIAEAVEQHYQPRFAGDNLPNGKVATAVALADKLETLVGIWGIGLIPTGDKDPYA
CCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LRRAALGILRMLMQYGLDVNELIQTAFDSFPKGLLNEKTPSETADFMQARLAVLLQNDYP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
QDIVAAVLAKQPRRLDDVVAKLQAVAAFKQLPEAAALAAANKRVQNLLKKADAALGEVNE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLQQDEEKALFAAAQGLQPKIAAAVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAA
HHHHCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCHHHEEHHHH
VKQNRLNLLNRLAEQMNAVADIALLGE
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA