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The map label for this gene is groEL [H]

Identifier: 161869236

GI number: 161869236

Start: 251151

End: 252785

Strand: Direct

Name: groEL [H]

Synonym: NMCC_0241

Alternate gene names: 161869236

Gene position: 251151-252785 (Clockwise)

Preceding gene: 161869235

Following gene: 161869237

Centisome position: 11.66

GC content: 54.68

Gene sequence:

>1635_bases
ATGGCAGCAAAAGACGTACAATTCGGCAATGAAGTCCGCCAAAAAATGGTAAACGGCGTGAACATTTTGGCAAACGCCGT
GCGCGTAACCTTGGGTCCCAAAGGCCGCAACGTGGTGGTTGACCGCGCTTTCGGCGGCCCGCACATCACCAAAGACGGCG
TAACCGTCGCCAAAGAAATCGAACTGAAAGACAAGTTTGAAAATATGGGCGCGCAAATGGTGAAAGAAGTCGCGTCCAAA
ACCAACGACGTGGCGGGCGACGGTACGACTACCGCCACCGTATTGGCGCAATCCATCGTTGCCGAAGGTATGAAATACGT
TACCGCCGGTATGAACCCGACCGACCTGAAACGCGGTATCGACAAAGCCGTTGCCGCTTTGGTTGAAGAGCTGAAAAACA
TCGCCAAACCTTGCGACACTTCCAAAGAAATCGCCCAAGTCGGCTCGATTTCCGCCAACTCTGACGAACAAGTCGGCGCG
ATTATCGCCGAAGCGATGGAAAAAGTCGGCAAAGAAGGCGTGATTACCGTTGAAGACGGCAAATCTTTGGAAAACGAGCT
GGACGTGGTTGAAGGTATGCAGTTCGACCGCGGCTACTTGTCTCCTTACTTCATCAACGACGCTGAAAAACAAATCGCTG
CTTTGGACAATCCGTTTGTATTGTTGTTCGACAAAAAAATCAGCAACATCCGCGACCTGCTGCCTGTTTTGGAACAAGTG
GCAAAAGCCAGCCGTCCGCTGTTGATTATCGCTGAAGACGTAGAAGGCGAAGCCTTGGCGACTTTGGTCGTGAACAACAT
CCGAGGCATCCTGAAAACCGTTGCCGTCAAAGCCCCTGGCTTCGGCGACCGCCGCAAAGCGATGTTGCAAGACATCGCCA
TCCTGACCGGCGGCGTGGTGATTTCCGAAGAAGTCGGTCTGTCTTTGGAAAAAGCGACTTTGGACGACTTGGGTCAAGCC
AAACGCATCGAAATCGGTAAAGAAAACACCACCATCATCGACGGCTTTGGCGACGCAGCCCAAATCGAAGCGCGTGTTGC
CGAAATCCGCCAACAAATCGAAACCGCAACCAGCGATTACGACAAAGAAAAACTGCAAGAGCGCGTGGCTAAATTGGCAG
GCGGCGTGGCAGTGATCAAAGTCGGCGCGGCTACCGAAGTCGAAATGAAAGAGAAAAAAGACCGCGTGGAAGACGCGTTG
CACGCCACCCGCGCTGCCGTTGAAGAAGGCGTGGTTGCAGGCGGCGGCGTAGCCCTGTTGCGCGCCCGTGCCGCTTTGGA
AAACCTGCACACCGGCAATGCCGACCAAGACGCAGGCGTACAAATCGTCTTGCGTGCCGTTGAGTCTCCGCTGCGCCAAA
TCGTTGCCAACGCAGGCGGCGAACCTAGCGTGGTGGTGAACAAAGTGTTGGAAGGCAAAGGCAACTACGGTTACAACGCA
GGCTCCGGCGAATACGGCGACATGATCGAAATGGGCGTACTCGACCCCGCCAAAGTAACCCGTTCCGCGCTGCAACACGC
CGCATCTATCGCCGGCTTGATGCTGACCACTGATTGCATGATCGCTGAAATCCCCGAAGACAAACCGGCTGTGCCTGATA
TGGGCGGCATGGGTGGTATGGGCGGCATGATGTAA

Upstream 100 bases:

>100_bases
TGAATAAGTGCGAATGATGATGCCGTCTGAAACGGCAAACCGCCTTCAGACGGCATAAACGGTTTTATCAGACAGTTTTA
ATGATTTTTGGAGAATTGAA

Downstream 100 bases:

>100_bases
GCAATGCCGTCTGAAGCTTTCAGATAAAAAACCGCACGGTCAACGCCGTGCGGTTTTTTTTGCGAATAAGTGCGGCTAAG
GCGCAAACCTTAAAATTGCC

Product: chaperonin GroEL

Products: NA

Alternate protein names: 63 kDa stress protein; GSP63; GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAFGGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASK
TNDVAGDGTTTATVLAQSIVAEGMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNPFVLLFDKKISNIRDLLPVLEQV
AKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAVKAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQA
KRIEIGKENTTIIDGFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDAL
HATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESPLRQIVANAGGEPSVVVNKVLEGKGNYGYNA
GSGEYGDMIEMGVLDPAKVTRSALQHAASIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM

Sequences:

>Translated_544_residues
MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAFGGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASK
TNDVAGDGTTTATVLAQSIVAEGMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNPFVLLFDKKISNIRDLLPVLEQV
AKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAVKAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQA
KRIEIGKENTTIIDGFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDAL
HATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESPLRQIVANAGGEPSVVVNKVLEGKGNYGYNA
GSGEYGDMIEMGVLDPAKVTRSALQHAASIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM
>Mature_543_residues
AAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAFGGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKT
NDVAGDGTTTATVLAQSIVAEGMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGAI
IAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNPFVLLFDKKISNIRDLLPVLEQVA
KASRPLLIIAEDVEGEALATLVVNNIRGILKTVAVKAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAK
RIEIGKENTTIIDGFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALH
ATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESPLRQIVANAGGEPSVVVNKVLEGKGNYGYNAG
SGEYGDMIEMGVLDPAKVTRSALQHAASIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=52.1821631878558, Blast_Score=553, Evalue=1e-157,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=52.1821631878558, Blast_Score=553, Evalue=1e-157,
Organism=Homo sapiens, GI38455427, Length=170, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=75.0943396226415, Blast_Score=801, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=53.3081285444234, Blast_Score=556, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI193210679, Length=211, Percent_Identity=52.60663507109, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=53.6259541984733, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322446, Length=564, Percent_Identity=21.4539007092199, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322524, Length=585, Percent_Identity=22.9059829059829, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24641193, Length=526, Percent_Identity=52.4714828897338, Blast_Score=554, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24641191, Length=526, Percent_Identity=52.4714828897338, Blast_Score=554, Evalue=1e-158,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=50, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=50, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=50, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI17864606, Length=542, Percent_Identity=44.6494464944649, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24584129, Length=533, Percent_Identity=37.8986866791745, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI19921262, Length=533, Percent_Identity=37.8986866791745, Blast_Score=357, Evalue=8e-99,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57438; Mature: 57306

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAFGGPHITKDGVTVAKEI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCHHHHHH
ELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAEGMKYVTAGMNPTDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHH
DKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGAIIAEAMEKVGKEGVITVEDG
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
KSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNPFVLLFDKKISNIRDLLPVLEQV
CCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHH
AKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAVKAPGFGDRRKAMLQDIAILTGGVV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEE
ISEEVGLSLEKATLDDLGQAKRIEIGKENTTIIDGFGDAAQIEARVAEIRQQIETATSDY
EEHHHCCEEHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
DKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALHATRAAVEEGVVAGGGVALL
CHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RARAALENLHTGNADQDAGVQIVLRAVESPLRQIVANAGGEPSVVVNKVLEGKGNYGYNA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
GSGEYGDMIEMGVLDPAKVTRSALQHAASIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGM
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCCCCCCC
GGMM
CCCC
>Mature Secondary Structure 
AAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAFGGPHITKDGVTVAKEI
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCHHHHHH
ELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAEGMKYVTAGMNPTDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHH
DKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGAIIAEAMEKVGKEGVITVEDG
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
KSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNPFVLLFDKKISNIRDLLPVLEQV
CCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHH
AKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAVKAPGFGDRRKAMLQDIAILTGGVV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEE
ISEEVGLSLEKATLDDLGQAKRIEIGKENTTIIDGFGDAAQIEARVAEIRQQIETATSDY
EEHHHCCEEHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
DKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALHATRAAVEEGVVAGGGVALL
CHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RARAALENLHTGNADQDAGVQIVLRAVESPLRQIVANAGGEPSVVVNKVLEGKGNYGYNA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
GSGEYGDMIEMGVLDPAKVTRSALQHAASIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGM
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCCCCCCC
GGMM
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7746149 [H]