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The map label for this gene is trmD [H]

Identifier: 161525402

GI number: 161525402

Start: 2446676

End: 2447470

Strand: Reverse

Name: trmD [H]

Synonym: Bmul_2232

Alternate gene names: 161525402

Gene position: 2447470-2446676 (Counterclockwise)

Preceding gene: 161525403

Following gene: 161525401

Centisome position: 70.97

GC content: 67.17

Gene sequence:

>795_bases
ATGAACCAGGTCACGGAGCGCGCGGTGCAGTTCGATGTCGTCACGCTCTTTCCCGAAATGTTCCGCGCGCTGACCGACTG
GGGTATCACCAGCCGGGCCGTGAAGCAGGGGCGCTTCGGGCTGCGTACCTGGAACCCGCGTGATTTCACGACGGACAACT
ACCGCACGGTCGACGATCGTCCGTACGGCGGCGGTCCGGGCATGGTGATGCTGGCCAGGCCGCTCGAGGCCGCGATCGGT
GCCGCGAAGGCGGCGCAGGCGGAGCAGGGCATCGCGAGCACGCGCGTCGTGATGATGTCGCCGCAGGGCGCGCCGCTCAC
GCACGAACGTGTGACGCGAATGGCGCAGGAGCCCGGCGTCGTCGTGCTGTGCGGCCGTTACGAGGCGATCGATCAACGGT
TGCTCGACCGCTGCGTCGACGAGGAGATCAGTCTCGGCGATTTCGTGCTGTCGGGCGGCGAACTGCCGGCAATGGCGATG
ATGGACGCAGTCGTGCGACTGCTGCCGGGCGTGCTCAACGATGCGCAGTCGGCCGTGCAGGACAGCTTCGTCGACGGCCT
GCTCGATTGTCCGCACTACACGCGTCCCGAGGAGTACGAGGGTATGCGCGTGCCGGACGTGCTGCTCGGCGGACATCATG
CCGAAATCGAGCGGTGGCGGCGCCAGGAAGCATTGAGAAACACATGGCGGAAGCGGCCGGACCTGATCGTCCGGGCGCGC
CGCGAGAAGTTGCTGAGCCGTGCCGACGAGGCATGGCTTGCGAACCTCGCACGCGAAGCGAAGGACGCCTCCTGA

Upstream 100 bases:

>100_bases
GCGGCTGCCGAGCGGCTGATCCCGTTCGTCGGCGTGTATGTCAAAACGGTAGACCAGGCGGCGCGTCGCATCGTCGTCGA
CTGGGAAGCCGATTACTGAA

Downstream 100 bases:

>100_bases
GGCGGCATCGCGGGCGGGAAATGGCGGTGCCGTACATGCGTGTGCGGCGCCTGATGTGAATCCATCCTCTATCGGGGCCA
GGCCTGGAAGCAGGCGGGCG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNQVTERAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLARPLEAAIG
AAKAAQAEQGIASTRVVMMSPQGAPLTHERVTRMAQEPGVVVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAM
MDAVVRLLPGVLNDAQSAVQDSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALRNTWRKRPDLIVRAR
REKLLSRADEAWLANLAREAKDAS

Sequences:

>Translated_264_residues
MNQVTERAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLARPLEAAIG
AAKAAQAEQGIASTRVVMMSPQGAPLTHERVTRMAQEPGVVVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAM
MDAVVRLLPGVLNDAQSAVQDSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALRNTWRKRPDLIVRAR
REKLLSRADEAWLANLAREAKDAS
>Mature_264_residues
MNQVTERAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLARPLEAAIG
AAKAAQAEQGIASTRVVMMSPQGAPLTHERVTRMAQEPGVVVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAM
MDAVVRLLPGVLNDAQSAVQDSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALRNTWRKRPDLIVRAR
REKLLSRADEAWLANLAREAKDAS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=56.25, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29562; Mature: 29562

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVTERAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLARPLEAAIGAAKAAQAEQGIASTRVVMMSPQGAPLTHERVTRMAQEPGV
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCE
VVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAMMDAVVRLLPGVLNDAQSAVQ
EEEECCHHHHHHHHHHHHHHCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALRNTWRKRPDLIVRAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
REKLLSRADEAWLANLAREAKDAS
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQVTERAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLARPLEAAIGAAKAAQAEQGIASTRVVMMSPQGAPLTHERVTRMAQEPGV
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCE
VVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAMMDAVVRLLPGVLNDAQSAVQ
EEEECCHHHHHHHHHHHHHHCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFVDGLLDCPHYTRPEEYEGMRVPDVLLGGHHAEIERWRRQEALRNTWRKRPDLIVRAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
REKLLSRADEAWLANLAREAKDAS
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA