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The map label for this gene is thyA [H]

Identifier: 161525388

GI number: 161525388

Start: 2434183

End: 2435154

Strand: Reverse

Name: thyA [H]

Synonym: Bmul_2218

Alternate gene names: 161525388

Gene position: 2435154-2434183 (Counterclockwise)

Preceding gene: 161525389

Following gene: 161525385

Centisome position: 70.62

GC content: 64.61

Gene sequence:

>972_bases
ATGAAACAGTATCTCGACCTCGTTCGCACCATCCTCGACACCGGCACCTGGCAGGAGAACCGCACGGGCATCCGCACCAT
CAGCATGCCGGGCGCGATGTTGCGGTTCGACCTGCAGCAGGGCTTCCCGGCCGTCACGACGAAGAAACTGGCGTTCAAGT
CCGCGATCGGCGAGCTGATCGGCTTTCTGCGCGCATCGCGCAGCGCGGCCGATTTCCGCGCGCTCGGCTGCAAGGTCTGG
GACGCCAACGCGAACGAGAACGCGCAATGGCTCGCGAATCCGTACCGGCAGGGCGTCGACGATCTGGGCGACGTCTACGG
CGTGCAGTGGCGCCAGTGGCCTGGCTATAAGGTGCTCGACGCGCACGCGGACGCGCAGCTCGCCGACGCGCAGCGCCGCG
GTTTCCGGATCGTCACGCGCTTCGACGAAGACGGCGCGCAGAAGGTGCTGCTGTACAAGGCGATCGATCAGCTGCGTCAG
TGTCTCGACACGATCATGGAGAATCCCGCCGATCGACGCATTCTGTTTCATGCGTGGAATCCGGCCGTCCTCGACCAGAT
CGCGCTGCCGGCCTGCCATCTGCTGTACCAGTTCCTGCCGAACGTCGCGAAGCGCGAAATCTCGCTGTGCCTCTATATCC
GCAGCAACGACGTCGGGCTCGGCACGCCGTTCAACCTGACCGAAGGCGCGGCGCTGCTGTCGCTCGTCGGCCGATTGACG
GGCTATACGCCTCGCTGGTTCACGTACTTCATCGGCGATGCGCATATCTACGAGAACCAGCTCGACATGCTGCAGCAGCA
GCTCAAGCGCGAGCCGTACGAAAGCCCGCGGCTCGTGATCGCCGATCGCGTGCCGGAATACGCGAAAACCGGCGTCTACG
AGCCGGAATGGCTCGAACGCGTCGAGCCGTCGGATTTCTCGCTGGTCGGCTACCGACACCATGAGCCGCTGACCGCGCCG
ATGGCGGTCTGA

Upstream 100 bases:

>100_bases
GCGACACGCTCGCGCCGTTTTCGCCGCCGCCGCCGGAGCTTCCTCGCCACGCTTGCTAAAATCTGCGGGTACGGCCGCCC
TCGCGGCCGAAGGAACCCGC

Downstream 100 bases:

>100_bases
CGGCCCGGCGGCCGGCCCGGCCGTTCCGCGCGGCAACGACGAAGGCCCGACCGGTGTATGCCGGTCGGGCCTTCGTCGTT
TGGGCGGGCGCGCCGCTCAG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELIGFLRASRSAADFRALGCKVW
DANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLDAHADAQLADAQRRGFRIVTRFDEDGAQKVLLYKAIDQLRQ
CLDTIMENPADRRILFHAWNPAVLDQIALPACHLLYQFLPNVAKREISLCLYIRSNDVGLGTPFNLTEGAALLSLVGRLT
GYTPRWFTYFIGDAHIYENQLDMLQQQLKREPYESPRLVIADRVPEYAKTGVYEPEWLERVEPSDFSLVGYRHHEPLTAP
MAV

Sequences:

>Translated_323_residues
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELIGFLRASRSAADFRALGCKVW
DANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLDAHADAQLADAQRRGFRIVTRFDEDGAQKVLLYKAIDQLRQ
CLDTIMENPADRRILFHAWNPAVLDQIALPACHLLYQFLPNVAKREISLCLYIRSNDVGLGTPFNLTEGAALLSLVGRLT
GYTPRWFTYFIGDAHIYENQLDMLQQQLKREPYESPRLVIADRVPEYAKTGVYEPEWLERVEPSDFSLVGYRHHEPLTAP
MAV
>Mature_323_residues
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELIGFLRASRSAADFRALGCKVW
DANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLDAHADAQLADAQRRGFRIVTRFDEDGAQKVLLYKAIDQLRQ
CLDTIMENPADRRILFHAWNPAVLDQIALPACHLLYQFLPNVAKREISLCLYIRSNDVGLGTPFNLTEGAALLSLVGRLT
GYTPRWFTYFIGDAHIYENQLDMLQQQLKREPYESPRLVIADRVPEYAKTGVYEPEWLERVEPSDFSLVGYRHHEPLTAP
MAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=321, Percent_Identity=36.1370716510903, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1789191, Length=323, Percent_Identity=34.6749226006192, Blast_Score=177, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI71993377, Length=277, Percent_Identity=34.6570397111913, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324648, Length=274, Percent_Identity=31.021897810219, Blast_Score=136, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17137556, Length=321, Percent_Identity=35.5140186915888, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36823; Mature: 36823

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELI
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHEEEEHHCCCCCHHHHHHHHHHHHHHHH
GFLRASRSAADFRALGCKVWDANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLD
HHHHHCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHCCCEECCCCCEEEE
AHADAQLADAQRRGFRIVTRFDEDGAQKVLLYKAIDQLRQCLDTIMENPADRRILFHAWN
CCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PAVLDQIALPACHLLYQFLPNVAKREISLCLYIRSNDVGLGTPFNLTEGAALLSLVGRLT
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GYTPRWFTYFIGDAHIYENQLDMLQQQLKREPYESPRLVIADRVPEYAKTGVYEPEWLER
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCCCHHHHHH
VEPSDFSLVGYRHHEPLTAPMAV
CCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELI
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHEEEEHHCCCCCHHHHHHHHHHHHHHHH
GFLRASRSAADFRALGCKVWDANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLD
HHHHHCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHCCCEECCCCCEEEE
AHADAQLADAQRRGFRIVTRFDEDGAQKVLLYKAIDQLRQCLDTIMENPADRRILFHAWN
CCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PAVLDQIALPACHLLYQFLPNVAKREISLCLYIRSNDVGLGTPFNLTEGAALLSLVGRLT
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GYTPRWFTYFIGDAHIYENQLDMLQQQLKREPYESPRLVIADRVPEYAKTGVYEPEWLER
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCCCHHHHHH
VEPSDFSLVGYRHHEPLTAPMAV
CCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]