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The map label for this gene is argT [H]

Identifier: 161525288

GI number: 161525288

Start: 2330363

End: 2331157

Strand: Reverse

Name: argT [H]

Synonym: Bmul_2118

Alternate gene names: 161525288

Gene position: 2331157-2330363 (Counterclockwise)

Preceding gene: 161525289

Following gene: 161525285

Centisome position: 67.6

GC content: 65.28

Gene sequence:

>795_bases
ATGAAGTGGGATTGGCGTAAAGTGGCCGCGCACGCGGTAGTGGCGGCATCGGCGGTAGCGGCAGGCAGCGCGTTGGCGGC
GGATCTGAAGGAGATCCGGTTCGGCGTCGAGGCCTCGTATGCGCCGTTCGAATACAAGACGCCCGACGGCAAGCTGACCG
GCTTCGACATCGACATCGGCAACGCGGTCTGCGCGAAGCTGAAGGCGAAGTGCGTGTGGGTCGAGAACGACTTCGACGGC
CTGATTCCCGCGCTGCAGGCGCGCAAGTTCGACGCGATCAACTCGGACATGACGATCACCGATCAGCGCAAGCACGCGAT
CGCCTTCACCGATCCGATCTACACGATCCCGAACCAGCTGATCGCGAAGCAAGGCAGCGGCCTGCAGCCGACGCCGCAAT
CGCTGAAGGGCAAGCGCGTCGGCGTGCTGCAGGGCACGATCCAGGAAGCGTACGCGAAGAAGAAGTGGGCGCCGGCCGGC
GTCGAAGTCGTGCCGTACCAGACGCAGGATCTCGCCTACGCCGACCTGAAGTCGGGCCGTCTCGACGCGACGTTCCAGGA
TTCGGAAGCGGGCGCGAAGGGCTTCCTGTCGAAGCCGCAAGGCCAGGGCTTCGCGTTCGCGGGCGGCACCGTCAGCGATG
CCGAGATCCTCGGCTCGGGCGTCGGCTTCGGCCTGCGCAAGAACGACGCGGCGCTGAAGACCGCGCTCGATCAGGCGCTG
AAGGAACTGAAGGCCGACGGCACGATCGACTCGCTCGCGAAGAAGTACTTCAGCGTGCCCGTCACGCTCAAGTAA

Upstream 100 bases:

>100_bases
CGCATCACGGCGCGTCGTCGCGGTTGCCGTCGTCATCCGGCCCTGCAATGATGCATGCGCCCGTAGTTCCGGAACATTCG
AGTATCGAGGAGAACACCTG

Downstream 100 bases:

>100_bases
GCGCGTTTCGATTCCGTCGCTTTTCGCCAGCCGGCCGCATCATGCGGCCGGGTTGCTTTTGGGCGGCCGCTACGCGGGGA
AGAACCGGCTCAGCTCGCGC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKWDWRKVAAHAVVAASAVAAGSALAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIGNAVCAKLKAKCVWVENDFDG
LIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQLIAKQGSGLQPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAG
VEVVPYQTQDLAYADLKSGRLDATFQDSEAGAKGFLSKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
KELKADGTIDSLAKKYFSVPVTLK

Sequences:

>Translated_264_residues
MKWDWRKVAAHAVVAASAVAAGSALAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIGNAVCAKLKAKCVWVENDFDG
LIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQLIAKQGSGLQPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAG
VEVVPYQTQDLAYADLKSGRLDATFQDSEAGAKGFLSKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
KELKADGTIDSLAKKYFSVPVTLK
>Mature_264_residues
MKWDWRKVAAHAVVAASAVAAGSALAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIGNAVCAKLKAKCVWVENDFDG
LIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQLIAKQGSGLQPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAG
VEVVPYQTQDLAYADLKSGRLDATFQDSEAGAKGFLSKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
KELKADGTIDSLAKKYFSVPVTLK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=241, Percent_Identity=52.6970954356847, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=49.5689655172414, Blast_Score=253, Evalue=8e-69,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=35.1020408163265, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=33.8775510204082, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=32.824427480916, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787031, Length=251, Percent_Identity=32.6693227091633, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28142; Mature: 28142

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWDWRKVAAHAVVAASAVAAGSALAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEECC
NAVCAKLKAKCVWVENDFDGLIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQL
HHHHHHHEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCHHHCCHHH
IAKQGSGLQPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAGVEVVPYQTQDLAYADLKSGR
HHCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCCC
LDATFQDSEAGAKGFLSKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
CCCEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KELKADGTIDSLAKKYFSVPVTLK
HHHCCCCCHHHHHHHHHCCCCEEC
>Mature Secondary Structure
MKWDWRKVAAHAVVAASAVAAGSALAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEECC
NAVCAKLKAKCVWVENDFDGLIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQL
HHHHHHHEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCHHHCCHHH
IAKQGSGLQPTPQSLKGKRVGVLQGTIQEAYAKKKWAPAGVEVVPYQTQDLAYADLKSGR
HHCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHCCCC
LDATFQDSEAGAKGFLSKPQGQGFAFAGGTVSDAEILGSGVGFGLRKNDAALKTALDQAL
CCCEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KELKADGTIDSLAKKYFSVPVTLK
HHHCCCCCHHHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]