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The map label for this gene is ywbI [H]

Identifier: 161525235

GI number: 161525235

Start: 2258747

End: 2259703

Strand: Direct

Name: ywbI [H]

Synonym: Bmul_2065

Alternate gene names: 161525235

Gene position: 2258747-2259703 (Clockwise)

Preceding gene: 161525231

Following gene: 161525239

Centisome position: 65.5

GC content: 66.77

Gene sequence:

>957_bases
ATGCGCGCCGATCTCGCTCCCTTCCTGAACGACCGTCTCGACTGGAACCTGTTGCGTACCTATCTGGTGATCATGCAGGA
GCGCAGCGTGAGTCGCGCGGCCGCGCGGCTGCACGTGACGCAGCCCGCGGTGAGCCAGGCGTTGCGCCGGCTCGAGGACA
CGCTCGAGCGCCGCCTGATCGAGCGGCGCGGCGCGCACTTCGCGCCGACGCCGGCCGGCGAAGCCGTCTACCGGATCGCG
AGCGACATCTACGGCGGCATCTCGCGCCTCGAAACGGAGCTCGACGACAGCACGGCCGACCTCACCGGCTCGATCCGGTT
GTTGACCGTCAGCCGCATCGAGTCGCCCGTCTACGACGAATTCCTCGCGGATTTCCATCGCGCATATCCGCGCGTCGATC
TGCAGATCGAAGTGATGCGCTCGTCGGACATCCTGTCGTCGCTGCTGCAGAAGACCGCAACGGCCGGGCTCGGCCTGTGC
CGCACGCCGCACGACAAGCTCGAGATGCGCTGCTTCCTGCGCCAGCGCTATGCGATCTACTGCGGTCGCCATCATCGTCT
GTTCGGGCACACGCAGTTGCGGATGGAAGACCTGCTCGCCGAGAACTTCGTGTCGTTCACGAGCGACCAGATCGGCGACA
GCCTGTCGCCGCTCACGGTATTTCGCGATCAGAAGGGCTTCACCGGACGGATCGTCGCGTCGTCGCCGAGTCTCGAGGAA
GTGCGGCGGCTGATCTTCGCGGGCTACGGCATCGGCTGCCTGCCCGAGCACATCGTGCGCGACGACATCGCCCAGCAGCG
GCTGTGGCGGCTGCCGCCCGACGAAGGGCTCGTCGACGTCGACGTGTTCCTGCTGTGGCACCGCGACCGCAAGATGAATG
CGGCCGAGCTCGCGTTTCTCGAGGCGATGGAGCGCTGCATGCAGCGCTATTCGCTCGCGCAGCGGCTCGGGAAGTAG

Upstream 100 bases:

>100_bases
CTCCTTTATCGGCCGCCGCAATTCATTAGTCAAATTTCAAGTTATTATTCGGCGTATAAATTGAACTAATGTACGACGTA
CCCTGCCGTCCGGAGACCCG

Downstream 100 bases:

>100_bases
CGACGCAGGCGCGACGCAGTGCACACGCTGTAGGTAACTCGGGCCGCACGCATGCCGGCCCGATGCGCACCGTCAGGGCT
GCGCTTCGCCGCGCTCGAGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRADLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEDTLERRLIERRGAHFAPTPAGEAVYRIA
SDIYGGISRLETELDDSTADLTGSIRLLTVSRIESPVYDEFLADFHRAYPRVDLQIEVMRSSDILSSLLQKTATAGLGLC
RTPHDKLEMRCFLRQRYAIYCGRHHRLFGHTQLRMEDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDEGLVDVDVFLLWHRDRKMNAAELAFLEAMERCMQRYSLAQRLGK

Sequences:

>Translated_318_residues
MRADLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEDTLERRLIERRGAHFAPTPAGEAVYRIA
SDIYGGISRLETELDDSTADLTGSIRLLTVSRIESPVYDEFLADFHRAYPRVDLQIEVMRSSDILSSLLQKTATAGLGLC
RTPHDKLEMRCFLRQRYAIYCGRHHRLFGHTQLRMEDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDEGLVDVDVFLLWHRDRKMNAAELAFLEAMERCMQRYSLAQRLGK
>Mature_318_residues
MRADLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEDTLERRLIERRGAHFAPTPAGEAVYRIA
SDIYGGISRLETELDDSTADLTGSIRLLTVSRIESPVYDEFLADFHRAYPRVDLQIEVMRSSDILSSLLQKTATAGLGLC
RTPHDKLEMRCFLRQRYAIYCGRHHRLFGHTQLRMEDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDEGLVDVDVFLLWHRDRKMNAAELAFLEAMERCMQRYSLAQRLGK

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=288, Percent_Identity=27.4305555555556, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787530, Length=297, Percent_Identity=25.5892255892256, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787589, Length=121, Percent_Identity=32.2314049586777, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787601, Length=276, Percent_Identity=22.463768115942, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=289, Percent_Identity=24.2214532871972, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36447; Mature: 36447

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRADLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEDTLERRLI
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERRGAHFAPTPAGEAVYRIASDIYGGISRLETELDDSTADLTGSIRLLTVSRIESPVYDE
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCHHHHH
FLADFHRAYPRVDLQIEVMRSSDILSSLLQKTATAGLGLCRTPHDKLEMRCFLRQRYAIY
HHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
CGRHHRLFGHTQLRMEDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCEEEEECCCCHHH
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDEGLVDVDVFLLWHRDRKMNAAELAFL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHEEEEECCCCCCHHHHHHH
EAMERCMQRYSLAQRLGK
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRADLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEDTLERRLI
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERRGAHFAPTPAGEAVYRIASDIYGGISRLETELDDSTADLTGSIRLLTVSRIESPVYDE
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCHHHHH
FLADFHRAYPRVDLQIEVMRSSDILSSLLQKTATAGLGLCRTPHDKLEMRCFLRQRYAIY
HHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
CGRHHRLFGHTQLRMEDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCEEEEECCCCHHH
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDEGLVDVDVFLLWHRDRKMNAAELAFL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHEEEEECCCCCCHHHHHHH
EAMERCMQRYSLAQRLGK
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]