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The map label for this gene is carA [H]

Identifier: 161525200

GI number: 161525200

Start: 2225269

End: 2226411

Strand: Reverse

Name: carA [H]

Synonym: Bmul_2030

Alternate gene names: 161525200

Gene position: 2226411-2225269 (Counterclockwise)

Preceding gene: 161525201

Following gene: 161525199

Centisome position: 64.56

GC content: 66.32

Gene sequence:

>1143_bases
TTGCCGTCTTTTTCTCCCGCCTTGCTTGCACTTGCCGACGGCACGGTCTTTCGTGGTTATTCGATCGGGGCCGAAGGCCA
TACGATCGGTGAAGTCGTGTTCAACACCGCGATCACCGGCTATCAGGAAATCCTGACGGATCCGAGCTACGCGCGCCAGA
TCGTGACGCTCACCTATCCGCATATCGGCAACGTCGGCGTGAACGCCGAAGACGTCGAAGCCACGAAAGTCCATGCCGCG
GGCCTGATCATCCGCGATCTGCCCACGCTTGCCTCGAACTTCCGGATGGAGCGCACGCTCGGCGACTACCTGCGCGACGA
AGGCGTCGTCGCGATCGCCGGCATCGATACCCGCAAGCTGACCCGCATCCTGCGCGACAAGGGCGCGCAGAACGGCTGCA
TCCTCGCGGGCTCGGACGACGCAGCGAAGGCGATCGAACTCGCGCGCTCGTTCCCGGGCCTCGCCGGCATGGATCTCGCG
AAGGTCGTGTCGACGACGAAACCGTTCGAGTGGAAGCAGACGGAATGGCGTCTCGGCAGCGGCTACGGGATGCAGGAAGC
GCCGAAGTACCGCGTCGTCGCGTACGACTTCGGCGTCAAGTACAACATCCTGCGCATGCTCGCGGAGCGCGGCTGCCAGG
TCACCGTGCTGCCCGCGCAGGCGAGCGCGGCCGACGCGCTGGCGCTGAATCCGGACGGCATCTTCCTGTCGAACGGCCCC
GGCGATCCGGAGCCGTGCGACTACGCGATCGCGGCGACCAAGGAATTCATCGAGCGCGGCGTGCCGACCTTCGGCATCTG
CCTCGGCCACCAGATCATGGGCCTCGCGGTCGGCGCGAAAACGCTGAAGATGAAGACGGGCCATCACGGCGCGAACCACC
CGGTGAAGGATCTCGCCGACGGCCGCGTCGTGATCACGTCGCAGAACCACGGCTTCGCGGTCGACGCGGACTCGCTGCCG
GCCAACGCACGTGCGACGCACGTGTCGCTGTTCGACGGCACGCTGCAGGGCTTCGAGCTCACCGACAAGCCGGCGTTCTG
CTTCCAGGGTCACCCGGAAGCGTCGCCGGGCCCGCACGACATCGGCTACCTGTTCGACCGCTTCACCGCCCTGATGGACG
CGGCGAAGCAGCGCAGCGCCTGA

Upstream 100 bases:

>100_bases
AGGCTGCGTCCAGCTAGCGACCCCGCGCGCACGCCGCGCGGCGCTCGCGCGGCAGGGTAGACAGGTCGGCAGCAGGGTGT
TCCCCAAGGAGCCTCCCGTG

Downstream 100 bases:

>100_bases
ACGGCGCGCGCACGGAGGCTCGCATCATGTTCGGTCACGCACTCGGCATCACGGATCTCTGGACCTACGTGTTCGGCGTG
ATCTTCATCATCCTGCTGCC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTYPHIGNVGVNAEDVEATKVHAA
GLIIRDLPTLASNFRMERTLGDYLRDEGVVAIAGIDTRKLTRILRDKGAQNGCILAGSDDAAKAIELARSFPGLAGMDLA
KVVSTTKPFEWKQTEWRLGSGYGMQEAPKYRVVAYDFGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGIFLSNGP
GDPEPCDYAIAATKEFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLADGRVVITSQNHGFAVDADSLP
ANARATHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHDIGYLFDRFTALMDAAKQRSA

Sequences:

>Translated_380_residues
MPSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTYPHIGNVGVNAEDVEATKVHAA
GLIIRDLPTLASNFRMERTLGDYLRDEGVVAIAGIDTRKLTRILRDKGAQNGCILAGSDDAAKAIELARSFPGLAGMDLA
KVVSTTKPFEWKQTEWRLGSGYGMQEAPKYRVVAYDFGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGIFLSNGP
GDPEPCDYAIAATKEFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLADGRVVITSQNHGFAVDADSLP
ANARATHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHDIGYLFDRFTALMDAAKQRSA
>Mature_379_residues
PSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTYPHIGNVGVNAEDVEATKVHAAG
LIIRDLPTLASNFRMERTLGDYLRDEGVVAIAGIDTRKLTRILRDKGAQNGCILAGSDDAAKAIELARSFPGLAGMDLAK
VVSTTKPFEWKQTEWRLGSGYGMQEAPKYRVVAYDFGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGIFLSNGPG
DPEPCDYAIAATKEFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLADGRVVITSQNHGFAVDADSLPA
NARATHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHDIGYLFDRFTALMDAAKQRSA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=387, Percent_Identity=38.5012919896641, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI21361331, Length=382, Percent_Identity=36.3874345549738, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI169790915, Length=382, Percent_Identity=36.3874345549738, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=65.2519893899204, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1789760, Length=128, Percent_Identity=30.46875, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=383, Percent_Identity=39.1644908616188, Blast_Score=245, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=388, Percent_Identity=37.3711340206186, Blast_Score=247, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6324878, Length=377, Percent_Identity=38.1962864721485, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=39.5886889460154, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=39.5886889460154, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40635; Mature: 40503

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTYP
CCCCCCEEEEEECCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEECC
HIGNVGVNAEDVEATKVHAAGLIIRDLPTLASNFRMERTLGDYLRDEGVVAIAGIDTRKL
CCCCCCCCCCCCHHHHHHHHCEEEHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCHHHH
TRILRDKGAQNGCILAGSDDAAKAIELARSFPGLAGMDLAKVVSTTKPFEWKQTEWRLGS
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEECC
GYGMQEAPKYRVVAYDFGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGIFLSNGP
CCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEECCC
GDPEPCDYAIAATKEFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLAD
CCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCC
GRVVITSQNHGFAVDADSLPANARATHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHD
CEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCCCCCEECCCCCEEECCCCCCCCCCCH
IGYLFDRFTALMDAAKQRSA
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSFSPALLALADGTVFRGYSIGAEGHTIGEVVFNTAITGYQEILTDPSYARQIVTLTYP
CCCCCEEEEEECCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEECC
HIGNVGVNAEDVEATKVHAAGLIIRDLPTLASNFRMERTLGDYLRDEGVVAIAGIDTRKL
CCCCCCCCCCCCHHHHHHHHCEEEHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCHHHH
TRILRDKGAQNGCILAGSDDAAKAIELARSFPGLAGMDLAKVVSTTKPFEWKQTEWRLGS
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEECC
GYGMQEAPKYRVVAYDFGVKYNILRMLAERGCQVTVLPAQASAADALALNPDGIFLSNGP
CCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEECCC
GDPEPCDYAIAATKEFIERGVPTFGICLGHQIMGLAVGAKTLKMKTGHHGANHPVKDLAD
CCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCC
GRVVITSQNHGFAVDADSLPANARATHVSLFDGTLQGFELTDKPAFCFQGHPEASPGPHD
CEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCCCCCEECCCCCEEECCCCCCCCCCCH
IGYLFDRFTALMDAAKQRSA
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]