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The map label for this gene is gatA [H]

Identifier: 161525141

GI number: 161525141

Start: 2168856

End: 2170268

Strand: Reverse

Name: gatA [H]

Synonym: Bmul_1969

Alternate gene names: 161525141

Gene position: 2170268-2168856 (Counterclockwise)

Preceding gene: 161525142

Following gene: 161525139

Centisome position: 62.93

GC content: 71.48

Gene sequence:

>1413_bases
ATGACGACCACTCCGCTCTACGAACTCAGTGCCGCGACGCTCGTCGACGGCTATCGCCGCAAGACGCTCTCGCCCGTCGA
CGTCACGCAGGCCGTGCTGGCGCGCATCGACGAATGGGAGCCGCTGATCCGTGCCACCTATGCGCTCGATCGCGACGGCG
CACTCGCGATGGCGCGCGCCTCGGAAGCGCGCTGGCTGCGCGGCGAACCGGCCGGTCCGCTCGACGGCGTGCCGGTGACG
ATCAAGGAAAACATCGCGACGCGCGGCGTGCCGGTGCCGCTCGGCTGCGCGGCGACGGAACTGACGCCGGCTGCCGACGA
TTCGCCGCCGGCGGCACGGTTGCGCGACGCGGGCGCGGTATTCGTCAGCAAGACGACGATGCCGGACTTCGGGCTGATCG
GCGCCGCGGCGTCGAGCTTTCATCCGCTGACACGCAATCCGTGGGATCTGAGCCGCAATACGGGCGGCTCGAGTTCGGGG
GCCGGCGCGGCCGCGGCGGCCGGCTACGGGCCGCTGCATCTCGGTTCCGACATCGGCGGCTCGGTGCGCATTCCGGCCGC
GTTCTGCGGCGTGTTCGGCTTCAAGCCGAGCTTCGGCCGCGTGCCGGTCGACCTGCCGTATCCGGGCCGCGTGACGGGGC
CGCTGACGCGAACGGTGCAGGACGCGGCGCTCGCGATGCAGACGATCGCGTTGCCCGATGCGCGCGATCACATGAGCCTG
CCGTATCAGCCGATCGAATGGCTGAATCTCGAGCGTGATCCGAAAGGGCTGCGCATCGGTCTGTGGATCGATCCGGGCAA
CGGCGTGCAGGTTGCGGCCGAGGTGCGCGCGGCGATCGAGCGTGCGGCCGCCGCGTTTGCGGCGGCGGGCGCGACGGTGG
TGCCGGTGCGCCCGTGGCTCGCACGCGGCGCGCTCGAAGCGGTCGCGCGTTTCTGGGGCGCGCGGCTGCGCGGCACGCTG
GCCGCGTTGCCCGCTGCGCGGCGCGAGCTGGTCGCCGCGTTCGTGCGCCGCCGCGCGGAAGTCGTTGCGGACGCAACGGG
CGATACGATCTATGCGGCGTATGCGCAGATGCTCGCGCTGCGCGCGCAGACGGTCGCGGCGACAGGCGAGTTCGATTACG
TGCTGTCGCCGACGTTTCCGCAGCCGCCGTTCGCGGCCGAAGCGACCCATATCGACCAGGACGGGTTGCATCCGATCGAG
CAGGTCGTGTTTACGCTCGCGTTCAATCTGTCGGAGCAGCCGGCCGCGTCGATCAACTGCGGCTATACGCGCGACGGCTT
GCCGATCGGGCTGCAAATCGCCGGGCGCCGCTTCGACGATCTCGGCGTGCTGCAGCTCGCGCGCTGCTGGGAACAGATGG
ACACGGTGCGGCGCCCGTGGCCCGAGGCGGCGGCCGCACGCGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCGAGCAAGCGCGCGTGGCGGCGTGAGCGCATCGGCCGCTCACCACCGCATCGGCACGTTTCCTGTCACAGCGCCGTTC
GGCGCGTTCATCGAAAACTC

Downstream 100 bases:

>100_bases
ACGCCATGCGCGGCGTCACGCCGCGTCTCTCGCCGACGCGCGCGCGGACAGCGCCGTGTAGCGCGTCGGCGATACGCCGA
TGTACTCGCGAAACCCGCGG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTTTPLYELSAATLVDGYRRKTLSPVDVTQAVLARIDEWEPLIRATYALDRDGALAMARASEARWLRGEPAGPLDGVPVT
IKENIATRGVPVPLGCAATELTPAADDSPPAARLRDAGAVFVSKTTMPDFGLIGAAASSFHPLTRNPWDLSRNTGGSSSG
AGAAAAAGYGPLHLGSDIGGSVRIPAAFCGVFGFKPSFGRVPVDLPYPGRVTGPLTRTVQDAALAMQTIALPDARDHMSL
PYQPIEWLNLERDPKGLRIGLWIDPGNGVQVAAEVRAAIERAAAAFAAAGATVVPVRPWLARGALEAVARFWGARLRGTL
AALPAARRELVAAFVRRRAEVVADATGDTIYAAYAQMLALRAQTVAATGEFDYVLSPTFPQPPFAAEATHIDQDGLHPIE
QVVFTLAFNLSEQPAASINCGYTRDGLPIGLQIAGRRFDDLGVLQLARCWEQMDTVRRPWPEAAAARAAA

Sequences:

>Translated_470_residues
MTTTPLYELSAATLVDGYRRKTLSPVDVTQAVLARIDEWEPLIRATYALDRDGALAMARASEARWLRGEPAGPLDGVPVT
IKENIATRGVPVPLGCAATELTPAADDSPPAARLRDAGAVFVSKTTMPDFGLIGAAASSFHPLTRNPWDLSRNTGGSSSG
AGAAAAAGYGPLHLGSDIGGSVRIPAAFCGVFGFKPSFGRVPVDLPYPGRVTGPLTRTVQDAALAMQTIALPDARDHMSL
PYQPIEWLNLERDPKGLRIGLWIDPGNGVQVAAEVRAAIERAAAAFAAAGATVVPVRPWLARGALEAVARFWGARLRGTL
AALPAARRELVAAFVRRRAEVVADATGDTIYAAYAQMLALRAQTVAATGEFDYVLSPTFPQPPFAAEATHIDQDGLHPIE
QVVFTLAFNLSEQPAASINCGYTRDGLPIGLQIAGRRFDDLGVLQLARCWEQMDTVRRPWPEAAAARAAA
>Mature_469_residues
TTTPLYELSAATLVDGYRRKTLSPVDVTQAVLARIDEWEPLIRATYALDRDGALAMARASEARWLRGEPAGPLDGVPVTI
KENIATRGVPVPLGCAATELTPAADDSPPAARLRDAGAVFVSKTTMPDFGLIGAAASSFHPLTRNPWDLSRNTGGSSSGA
GAAAAAGYGPLHLGSDIGGSVRIPAAFCGVFGFKPSFGRVPVDLPYPGRVTGPLTRTVQDAALAMQTIALPDARDHMSLP
YQPIEWLNLERDPKGLRIGLWIDPGNGVQVAAEVRAAIERAAAAFAAAGATVVPVRPWLARGALEAVARFWGARLRGTLA
ALPAARRELVAAFVRRRAEVVADATGDTIYAAYAQMLALRAQTVAATGEFDYVLSPTFPQPPFAAEATHIDQDGLHPIEQ
VVFTLAFNLSEQPAASINCGYTRDGLPIGLQIAGRRFDDLGVLQLARCWEQMDTVRRPWPEAAAARAAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=524, Percent_Identity=30.3435114503817, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI195972892, Length=249, Percent_Identity=31.7269076305221, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI166795287, Length=253, Percent_Identity=32.8063241106719, Blast_Score=73, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=240, Percent_Identity=32.0833333333333, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=238, Percent_Identity=30.2521008403361, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17537465, Length=236, Percent_Identity=29.6610169491525, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=234, Percent_Identity=32.4786324786325, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=254, Percent_Identity=31.496062992126, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=254, Percent_Identity=31.496062992126, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=302, Percent_Identity=28.476821192053, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=184, Percent_Identity=37.5, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319685, Length=179, Percent_Identity=32.4022346368715, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=24.3027888446215, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24648435, Length=482, Percent_Identity=25.5186721991701, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648437, Length=482, Percent_Identity=25.5186721991701, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45550774, Length=482, Percent_Identity=25.5186721991701, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648441, Length=412, Percent_Identity=25.7281553398058, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24648439, Length=412, Percent_Identity=25.7281553398058, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078093, Length=477, Percent_Identity=26.8343815513627, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652985, Length=297, Percent_Identity=29.96632996633, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19922090, Length=297, Percent_Identity=29.96632996633, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24652981, Length=297, Percent_Identity=29.96632996633, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24652983, Length=297, Percent_Identity=29.96632996633, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648113, Length=497, Percent_Identity=27.7665995975855, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21356731, Length=245, Percent_Identity=28.1632653061224, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49770; Mature: 49639

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPLYELSAATLVDGYRRKTLSPVDVTQAVLARIDEWEPLIRATYALDRDGALAMARA
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEHHC
SEARWLRGEPAGPLDGVPVTIKENIATRGVPVPLGCAATELTPAADDSPPAARLRDAGAV
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEE
FVSKTTMPDFGLIGAAASSFHPLTRNPWDLSRNTGGSSSGAGAAAAAGYGPLHLGSDIGG
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCCCC
SVRIPAAFCGVFGFKPSFGRVPVDLPYPGRVTGPLTRTVQDAALAMQTIALPDARDHMSL
CEECHHHHHHHHCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCC
PYQPIEWLNLERDPKGLRIGLWIDPGNGVQVAAEVRAAIERAAAAFAAAGATVVPVRPWL
CCCCHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHH
ARGALEAVARFWGARLRGTLAALPAARRELVAAFVRRRAEVVADATGDTIYAAYAQMLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAQTVAATGEFDYVLSPTFPQPPFAAEATHIDQDGLHPIEQVVFTLAFNLSEQPAASINC
HHHHHEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEC
GYTRDGLPIGLQIAGRRFDDLGVLQLARCWEQMDTVRRPWPEAAAARAAA
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
TTTPLYELSAATLVDGYRRKTLSPVDVTQAVLARIDEWEPLIRATYALDRDGALAMARA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEHHC
SEARWLRGEPAGPLDGVPVTIKENIATRGVPVPLGCAATELTPAADDSPPAARLRDAGAV
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEE
FVSKTTMPDFGLIGAAASSFHPLTRNPWDLSRNTGGSSSGAGAAAAAGYGPLHLGSDIGG
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCCCC
SVRIPAAFCGVFGFKPSFGRVPVDLPYPGRVTGPLTRTVQDAALAMQTIALPDARDHMSL
CEECHHHHHHHHCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCC
PYQPIEWLNLERDPKGLRIGLWIDPGNGVQVAAEVRAAIERAAAAFAAAGATVVPVRPWL
CCCCHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHH
ARGALEAVARFWGARLRGTLAALPAARRELVAAFVRRRAEVVADATGDTIYAAYAQMLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAQTVAATGEFDYVLSPTFPQPPFAAEATHIDQDGLHPIEQVVFTLAFNLSEQPAASINC
HHHHHEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEC
GYTRDGLPIGLQIAGRRFDDLGVLQLARCWEQMDTVRRPWPEAAAARAAA
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA