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The map label for this gene is cyaE [H]

Identifier: 161525127

GI number: 161525127

Start: 2152724

End: 2154154

Strand: Reverse

Name: cyaE [H]

Synonym: Bmul_1955

Alternate gene names: 161525127

Gene position: 2154154-2152724 (Counterclockwise)

Preceding gene: 161525128

Following gene: 161525126

Centisome position: 62.47

GC content: 70.02

Gene sequence:

>1431_bases
ATGATGGCGCCGCGTATCGCCGCCGTGCTGATCGCCGTCGCGTGGGCGACGGCGGCGCCGCGCGCGCACGCGCAGGCGTT
CGATGTCTATTCGACGCGTGCCGACGTGCCGCCGGGCCCATCGGCGCCGCTCGCGTCGGCCACGTCGTGCGACGGCGCGC
AGGCGCCGGATCGGCCGCTCGCGCTCGAAGACGCGATCCTGCTCGCGATCTGCTCGCATCCGCAGCTGCGCCGCACGTGG
TCCGATGCGCGCGCGGCGGCGGCCGCGGTCGGCATCGCGAAGGCCGCGTACTGGCCGACGCTGAACGCGACGGGCGGTAT
CGAGCGCGACGATCTGACGATGAAGTACGACGCGGGCATTACGCAGGATCAGCGCAGCACGAGCCGCTACGGGATGGTGA
ACCTGAGCTGGGTGCTGTTCGATTTCGGCAAGCGCGGCGCGTCGCTCGATCGCGCACGCGCGCTGCTGCGCGCCGCGAAC
GCCGCACATGACGATGCGCTGCAGACCGTTTTCTACGACGCCGCGCACGCGTACTACGCGTTGCGCGATGCGCGCGCGGC
GCTCGACGCGGCACGTGCGGTCGAACGCGCGGCGCAGGAAAGCCTGAACGAGGCGAGCGCGCGGCACGACGGCGGGGCGG
GCACGCTCGGCGACGCGCTGCAGGCGCAGACGACCTATCGCAAGGCGGTGCTCGACCGCGTCAGCGCGGAAGGCGACGTG
CAGACGGCGCTCGGCACGCTCGCGACGACGCTCGGCGTCGATGCCGATACGCCGCTGCAGATCGCCGACGGCGAGCCGTC
GCCCGATCGCGACGATTTCGCGCGCGGCGTTGCGGACCTGATCGCCGAAGCGAAGCGCCATCATCCGAAGCTGATCGCCG
CGCGAGAAAAGCTCGAAGCGGCACGCGACGAGGTGCGCGTCGTGCGCGCGCAGACGCGCCCGACGGTATCGCTGACGGGC
AGCGTCGCGCGCAACAACCCGTCGTATCAGCAGCAGGCCTCGCCGCTGCAGCTGCGCAGCAGCCACAGCAACTCGATCGG
CATCCAGATCTCGATTCCGCTGTTCGAAGGGTTCGCGTCCGGCTATCGCGTCGCGCAAGCGCAGGCGCAGGCCGACGCGC
AGGCGGCCGACGTGCGCAATACCGAACTGCAGGTGTCGCTCGACGTATGGAAGAGCTATCAGAGCCTGCAGACCGACACC
GCGAACCTCGACAACTCGCAGCACCTGCTCGACACCGCACTGCATTCGCTCGACATCGCACGCGGCCGCTACAAGGCAGG
TGTCGGCACGTTCACCGACTTGCTCAATGCGCAGTCGGCGCTCGCCGACGCGCGCCGGCAGCGCGTGCTTGCGGTGTCGA
AATGGCGTATCGCGCGACTGAAACTGGCCGAAAGCCTCGGGAGGTTGGGACTATGGAACGACGCGGGTTGA

Upstream 100 bases:

>100_bases
CGATGCGCGCGGCACGCTCGGCCGCGCAGCCGCCGTCGGCTGCGCCCGCCGCGCCCGCCGCGCGCACCGGCGGCGCGACC
GAACCGGTGGCGGGGCGATG

Downstream 100 bases:

>100_bases
ACGGTGCGCACGCCAGCGGCTGCGCGTTCCTGTTTCCGGGACAGGGCGCGTTCTACGCGAGCGCGATGCGCGAACTGGCC
GGCACGCACGCGAGCGTGCG

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MMAPRIAAVLIAVAWATAAPRAHAQAFDVYSTRADVPPGPSAPLASATSCDGAQAPDRPLALEDAILLAICSHPQLRRTW
SDARAAAAAVGIAKAAYWPTLNATGGIERDDLTMKYDAGITQDQRSTSRYGMVNLSWVLFDFGKRGASLDRARALLRAAN
AAHDDALQTVFYDAAHAYYALRDARAALDAARAVERAAQESLNEASARHDGGAGTLGDALQAQTTYRKAVLDRVSAEGDV
QTALGTLATTLGVDADTPLQIADGEPSPDRDDFARGVADLIAEAKRHHPKLIAAREKLEAARDEVRVVRAQTRPTVSLTG
SVARNNPSYQQQASPLQLRSSHSNSIGIQISIPLFEGFASGYRVAQAQAQADAQAADVRNTELQVSLDVWKSYQSLQTDT
ANLDNSQHLLDTALHSLDIARGRYKAGVGTFTDLLNAQSALADARRQRVLAVSKWRIARLKLAESLGRLGLWNDAG

Sequences:

>Translated_476_residues
MMAPRIAAVLIAVAWATAAPRAHAQAFDVYSTRADVPPGPSAPLASATSCDGAQAPDRPLALEDAILLAICSHPQLRRTW
SDARAAAAAVGIAKAAYWPTLNATGGIERDDLTMKYDAGITQDQRSTSRYGMVNLSWVLFDFGKRGASLDRARALLRAAN
AAHDDALQTVFYDAAHAYYALRDARAALDAARAVERAAQESLNEASARHDGGAGTLGDALQAQTTYRKAVLDRVSAEGDV
QTALGTLATTLGVDADTPLQIADGEPSPDRDDFARGVADLIAEAKRHHPKLIAAREKLEAARDEVRVVRAQTRPTVSLTG
SVARNNPSYQQQASPLQLRSSHSNSIGIQISIPLFEGFASGYRVAQAQAQADAQAADVRNTELQVSLDVWKSYQSLQTDT
ANLDNSQHLLDTALHSLDIARGRYKAGVGTFTDLLNAQSALADARRQRVLAVSKWRIARLKLAESLGRLGLWNDAG
>Mature_476_residues
MMAPRIAAVLIAVAWATAAPRAHAQAFDVYSTRADVPPGPSAPLASATSCDGAQAPDRPLALEDAILLAICSHPQLRRTW
SDARAAAAAVGIAKAAYWPTLNATGGIERDDLTMKYDAGITQDQRSTSRYGMVNLSWVLFDFGKRGASLDRARALLRAAN
AAHDDALQTVFYDAAHAYYALRDARAALDAARAVERAAQESLNEASARHDGGAGTLGDALQAQTTYRKAVLDRVSAEGDV
QTALGTLATTLGVDADTPLQIADGEPSPDRDDFARGVADLIAEAKRHHPKLIAAREKLEAARDEVRVVRAQTRPTVSLTG
SVARNNPSYQQQASPLQLRSSHSNSIGIQISIPLFEGFASGYRVAQAQAQADAQAADVRNTELQVSLDVWKSYQSLQTDT
ANLDNSQHLLDTALHSLDIARGRYKAGVGTFTDLLNAQSALADARRQRVLAVSKWRIARLKLAESLGRLGLWNDAG

Specific function: CyaE is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=410, Percent_Identity=24.1463414634146, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50903; Mature: 50903

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAPRIAAVLIAVAWATAAPRAHAQAFDVYSTRADVPPGPSAPLASATSCDGAQAPDRPL
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ALEDAILLAICSHPQLRRTWSDARAAAAAVGIAKAAYWPTLNATGGIERDDLTMKYDAGI
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
TQDQRSTSRYGMVNLSWVLFDFGKRGASLDRARALLRAANAAHDDALQTVFYDAAHAYYA
CCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LRDARAALDAARAVERAAQESLNEASARHDGGAGTLGDALQAQTTYRKAVLDRVSAEGDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
QTALGTLATTLGVDADTPLQIADGEPSPDRDDFARGVADLIAEAKRHHPKLIAAREKLEA
HHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARDEVRVVRAQTRPTVSLTGSVARNNPSYQQQASPLQLRSSHSNSIGIQISIPLFEGFAS
HHHHHHHHHHCCCCCEEEECCHHCCCCCHHHHCCCHHHHCCCCCCEEEEEEEHHHHHHHH
GYRVAQAQAQADAQAADVRNTELQVSLDVWKSYQSLQTDTANLDNSQHLLDTALHSLDIA
HHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RGRYKAGVGTFTDLLNAQSALADARRQRVLAVSKWRIARLKLAESLGRLGLWNDAG
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMAPRIAAVLIAVAWATAAPRAHAQAFDVYSTRADVPPGPSAPLASATSCDGAQAPDRPL
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ALEDAILLAICSHPQLRRTWSDARAAAAAVGIAKAAYWPTLNATGGIERDDLTMKYDAGI
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
TQDQRSTSRYGMVNLSWVLFDFGKRGASLDRARALLRAANAAHDDALQTVFYDAAHAYYA
CCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LRDARAALDAARAVERAAQESLNEASARHDGGAGTLGDALQAQTTYRKAVLDRVSAEGDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
QTALGTLATTLGVDADTPLQIADGEPSPDRDDFARGVADLIAEAKRHHPKLIAAREKLEA
HHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARDEVRVVRAQTRPTVSLTGSVARNNPSYQQQASPLQLRSSHSNSIGIQISIPLFEGFAS
HHHHHHHHHHCCCCCEEEECCHHCCCCCHHHHCCCHHHHCCCCCCEEEEEEEHHHHHHHH
GYRVAQAQAQADAQAADVRNTELQVSLDVWKSYQSLQTDTANLDNSQHLLDTALHSLDIA
HHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RGRYKAGVGTFTDLLNAQSALADARRQRVLAVSKWRIARLKLAESLGRLGLWNDAG
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]