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The map label for this gene is iolD [H]

Identifier: 161525065

GI number: 161525065

Start: 2071073

End: 2072989

Strand: Direct

Name: iolD [H]

Synonym: Bmul_1893

Alternate gene names: 161525065

Gene position: 2071073-2072989 (Clockwise)

Preceding gene: 161525064

Following gene: 161525066

Centisome position: 60.06

GC content: 70.68

Gene sequence:

>1917_bases
ATGTCCGAACCGACACTTCTTGACCGACAGACGATGACCCCTACCGTGAGACTGACCGTCAGCCAGGCGCTCGTGCGCTA
CCTGGCCGCGCTGCGCGCCGAAGTCGTCCAGCCGGACGGCCGTACCGACATCCTGCCGTACTGCGGCGGCGTGTTCGCGA
TCTTCGGGCACGGCAACGTGGCCGGGCTCGGCGAAGCGTTGCATGCCGAAAAAGACCGGCTGCCGACCTTGCGTGCGCAC
AACGAGCAAGGGATGGCCAATGCGGCCATCGCGTTCGCGAAAGCGAACTTCCGCCAGCGGATGATGGCCGTGACGTCGAG
CATCGGCCCCGGTGCGACCAACATGCTGACGTCGGCCGCGCTCGCGCATGTCGGCCGGCTGCCGCTGCTGCTGCTGCCCG
GCGACGTGTTCGTGTCGCGGCTGCCCGATCCCGTGCTGCAGCAGGTCGAGGATTTCGAACAGGGCGACGTCAGCGCGAAC
GACTGTTTCCGCCCCGTCACGCGCTACTTCGACCGCATTACGTCGCCCGAGCAGCTGCTCGTCGCGCTGCCGCGCGCGAT
CCAGGTGATGACCGATCCCGCGCAATGCGGCCCCGTCTGCCTCGCGCTGCCGCAGGACGTGCAGACCTTCGCGTACGACT
GGCCCGAGGATTTCTTCGCGCCGCCGCTGATCCGCATGCGGCGGCCGCCGGCCGACGCGCTCGAGCTCGCGGATGCGCTC
GACGTGCTGAAGGCCGCGAAAAAGCCGCTGATCGTCGCCGGCGGCGGCGTGCTGTACAGCCAGGCGTGGGACGCGCTGCG
CACGTTCGCCGACACGCACGGCGTGCCGGTCGCCGAATCGCAGGCCGGCAAGGGCAGTCTCGCGTGGGATCATCCGCTGA
ACCTCGGCTCGATCGGCGTGACGGGCTCGCCCGCCGCGAACCGCGCGGCCGCGCAGGCCGACGTCGTGTTCGCGGTCGGC
ACGCGGCTGCAGGATTTCACGACCGGCTCGCATGCGCTGTTCGGCCAGGCGACGCTGCTGAGCCTGAACGTGCAGCCGTT
CGATGCGGGCAAGAAGCGCGGCCGGCAGCTCGTCGCCGATGCGCGCACGGGGCTCGGCCAGCTGTCGGCCGCGCTCGCCG
GATGGCAGGCCGATCCGGCATGGACGGCGGCGAACCGCGATCAGGCCGCCGCGTGGAACGCGCGCGTCGCCGAGCTCACG
ACGCGCGTGCCCACCGACACGCTGCCGTACGACGCGGAAGTGATCGGCGCCGTGCGCGACTCCGCGGCGGACGCCGGACG
CGACAGCGCGCGCGACGATCTGGTCGTGTGCGCGGCCGGCACGCTGCCGGCCGAACTGCACAAGCTCTGGCGCAGCGGCC
TGCCCGGCAACTATCACATGGACTACGCGTATTCGTGCATGGGCTACGAGGTGGCCGGCGGCCTCGGCGCGAAGCTCGCG
CGGCCCGAGCGCGAGGTGATCGTGATCGTCGGCGACGGGTCATACATGATGCTCAACGCGGAGCTCGCGACCTCGGTGAT
GCTCGGCCGCAAGATCATCGTCGTGATCCTCGACAACCGCGGCTACGGCTGCATCGAGCGGCTGCAGCTGAACTGCGGCG
GCGCGAGCTTCAACAACATGCTCGACGACTGCGTGCCCGAAGGCGGCGAACGCTCGACGATCGACTTCGCGATGCATGCG
CGTTCGATGGGCGCCGAGGCCGTGCACGTGCGCGACGTCGGCGAGCTGCGCCGCGAGATGGCGCGTGCGCGCGCGGCCGC
CAAGAGCCAGGTGCTCGTGATCGACACGACGCACCGCCGCACGACCGAGGACGGCGGCGCGTGGTGGGAAGTCGCGGTGC
CGCAGGTGTCCGCGCGGCCCGACGTCGAACGCGCGCACCGCCGCTATCTCGACGACAAGACGCGGCAGCGGCGCTAG

Upstream 100 bases:

>100_bases
TGATCGACGAGGTCGCCGGCAATTTCGCGCAGCTCGTCGACGCCTGGCTCGGCCGCCGCGCGGCCGCGCGCGCCGCGGCC
GCATGAGGCCCGCCGCCGCG

Downstream 100 bases:

>100_bases
GCACGCGCCCTGCCCGCCCGTAGGACGACGACGACAACAACGGAACCAAGGAAGCCCGTCATGAGCTGGAACGTTCGCAT
CGGTATCAATCCCCTGTCGT

Product: thiamine pyrophosphate protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MSEPTLLDRQTMTPTVRLTVSQALVRYLAALRAEVVQPDGRTDILPYCGGVFAIFGHGNVAGLGEALHAEKDRLPTLRAH
NEQGMANAAIAFAKANFRQRMMAVTSSIGPGATNMLTSAALAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSAN
DCFRPVTRYFDRITSPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADAL
DVLKAAKKPLIVAGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGSIGVTGSPAANRAAAQADVVFAVG
TRLQDFTTGSHALFGQATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAALAGWQADPAWTAANRDQAAAWNARVAELT
TRVPTDTLPYDAEVIGAVRDSAADAGRDSARDDLVVCAAGTLPAELHKLWRSGLPGNYHMDYAYSCMGYEVAGGLGAKLA
RPEREVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGERSTIDFAMHA
RSMGAEAVHVRDVGELRREMARARAAAKSQVLVIDTTHRRTTEDGGAWWEVAVPQVSARPDVERAHRRYLDDKTRQRR

Sequences:

>Translated_638_residues
MSEPTLLDRQTMTPTVRLTVSQALVRYLAALRAEVVQPDGRTDILPYCGGVFAIFGHGNVAGLGEALHAEKDRLPTLRAH
NEQGMANAAIAFAKANFRQRMMAVTSSIGPGATNMLTSAALAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSAN
DCFRPVTRYFDRITSPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADAL
DVLKAAKKPLIVAGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGSIGVTGSPAANRAAAQADVVFAVG
TRLQDFTTGSHALFGQATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAALAGWQADPAWTAANRDQAAAWNARVAELT
TRVPTDTLPYDAEVIGAVRDSAADAGRDSARDDLVVCAAGTLPAELHKLWRSGLPGNYHMDYAYSCMGYEVAGGLGAKLA
RPEREVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGERSTIDFAMHA
RSMGAEAVHVRDVGELRREMARARAAAKSQVLVIDTTHRRTTEDGGAWWEVAVPQVSARPDVERAHRRYLDDKTRQRR
>Mature_637_residues
SEPTLLDRQTMTPTVRLTVSQALVRYLAALRAEVVQPDGRTDILPYCGGVFAIFGHGNVAGLGEALHAEKDRLPTLRAHN
EQGMANAAIAFAKANFRQRMMAVTSSIGPGATNMLTSAALAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSAND
CFRPVTRYFDRITSPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADALD
VLKAAKKPLIVAGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGSIGVTGSPAANRAAAQADVVFAVGT
RLQDFTTGSHALFGQATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAALAGWQADPAWTAANRDQAAAWNARVAELTT
RVPTDTLPYDAEVIGAVRDSAADAGRDSARDDLVVCAAGTLPAELHKLWRSGLPGNYHMDYAYSCMGYEVAGGLGAKLAR
PEREVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGERSTIDFAMHAR
SMGAEAVHVRDVGELRREMARARAAAKSQVLVIDTTHRRTTEDGGAWWEVAVPQVSARPDVERAHRRYLDDKTRQRR

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=28.6219081272085, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1786717, Length=513, Percent_Identity=25.7309941520468, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790104, Length=483, Percent_Identity=24.4306418219462, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788716, Length=487, Percent_Identity=24.6406570841889, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787096, Length=490, Percent_Identity=20.4081632653061, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323755, Length=588, Percent_Identity=25.6802721088435, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 68715; Mature: 68584

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPTLLDRQTMTPTVRLTVSQALVRYLAALRAEVVQPDGRTDILPYCGGVFAIFGHGNV
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEEECCCH
AGLGEALHAEKDRLPTLRAHNEQGMANAAIAFAKANFRQRMMAVTSSIGPGATNMLTSAA
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDCFRPVTRYFDRITSPEQLL
HHHHCCCCEEEECHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
VALPRAIQVMTDPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADAL
HHHHHHHHHCCCCHHCCCEEEECCCHHHHHHCCCCHHHCCCCHHHHCCCCHHHHHHHHHH
DVLKAAKKPLIVAGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGSIGV
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TGSPAANRAAAQADVVFAVGTRLQDFTTGSHALFGQATLLSLNVQPFDAGKKRGRQLVAD
CCCCCCCCHHHHHCEEEEECCCHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHCHHHHHH
ARTGLGQLSAALAGWQADPAWTAANRDQAAAWNARVAELTTRVPTDTLPYDAEVIGAVRD
HHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHH
SAADAGRDSARDDLVVCAAGTLPAELHKLWRSGLPGNYHMDYAYSCMGYEVAGGLGAKLA
HHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEECCEEEEECCCHHCCCCCCCCC
RPEREVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNM
CCCCEEEEEEECCCEEEEEHHHHHHHHHCCEEEEEEECCCCCCCCHHEEECCCCCCHHHH
LDDCVPEGGERSTIDFAMHARSMGAEAVHVRDVGELRREMARARAAAKSQVLVIDTTHRR
HHHHCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
TTEDGGAWWEVAVPQVSARPDVERAHRRYLDDKTRQRR
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHCHHHHCCC
>Mature Secondary Structure 
SEPTLLDRQTMTPTVRLTVSQALVRYLAALRAEVVQPDGRTDILPYCGGVFAIFGHGNV
CCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEEECCCH
AGLGEALHAEKDRLPTLRAHNEQGMANAAIAFAKANFRQRMMAVTSSIGPGATNMLTSAA
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDCFRPVTRYFDRITSPEQLL
HHHHCCCCEEEECHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
VALPRAIQVMTDPAQCGPVCLALPQDVQTFAYDWPEDFFAPPLIRMRRPPADALELADAL
HHHHHHHHHCCCCHHCCCEEEECCCHHHHHHCCCCHHHCCCCHHHHCCCCHHHHHHHHHH
DVLKAAKKPLIVAGGGVLYSQAWDALRTFADTHGVPVAESQAGKGSLAWDHPLNLGSIGV
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TGSPAANRAAAQADVVFAVGTRLQDFTTGSHALFGQATLLSLNVQPFDAGKKRGRQLVAD
CCCCCCCCHHHHHCEEEEECCCHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHCHHHHHH
ARTGLGQLSAALAGWQADPAWTAANRDQAAAWNARVAELTTRVPTDTLPYDAEVIGAVRD
HHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHH
SAADAGRDSARDDLVVCAAGTLPAELHKLWRSGLPGNYHMDYAYSCMGYEVAGGLGAKLA
HHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEECCEEEEECCCHHCCCCCCCCC
RPEREVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNM
CCCCEEEEEEECCCEEEEEHHHHHHHHHCCEEEEEEECCCCCCCCHHEEECCCCCCHHHH
LDDCVPEGGERSTIDFAMHARSMGAEAVHVRDVGELRREMARARAAAKSQVLVIDTTHRR
HHHHCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
TTEDGGAWWEVAVPQVSARPDVERAHRRYLDDKTRQRR
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA