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The map label for this gene is mdlB [H]

Identifier: 161524869

GI number: 161524869

Start: 1869318

End: 1870535

Strand: Direct

Name: mdlB [H]

Synonym: Bmul_1696

Alternate gene names: 161524869

Gene position: 1869318-1870535 (Clockwise)

Preceding gene: 161524867

Following gene: 161524873

Centisome position: 54.21

GC content: 67.32

Gene sequence:

>1218_bases
ATGCGTGTCGTCGATCGCGCCACACTTCGTCGCGACGCTGCGGCGCCCACCGACAAGCCGCCGCGCGTGCTGCGCAGCAT
GCTGAGCCTGCACGATTTCGAAGCACGCGCGCGCCGCGTGTTACCGCGTCCGATCTTCGGCTACGTGAGCGGTGCCGCCG
AAGACAATCGCACGCGCGACGACAACCGCAGCGTGTTCGACGAATTCGGCTTCACGACGCGCGTGCTGCGCAACGTGTCG
GCCCGCACGCAGGCGGTCGACCTGTTCGGGCAGCGGTTCGCCGCGCCTTTCGGTATCGCGCCGATGGGCATCAACGCGTT
GTCGGCCTATCGCGGCGACATCGTGCTCGCCCGCGCGGCGCAGGCGGCAGGCATCGCGTCGATCATGAGCGGCTCGTCGC
TGATCCCGCTCGAAGCGGTCGCGGCGGCAGCGCCGTCAACCTGGTTTCAGGCTTATCTTCCGGGCGATCCCGAACGCATC
GCCGCGCTGCTCGAGCGCGTCGCGCGTGCGGGATACCGGACGCTCGTCGTGACCGTCGACATTCCCGTTGCGGCGAATCG
CGAGAACAACGTCCGTACCGGTTTCTCCACGCCGCTGCGTCCGAGCCTGCGTCTCGCGTGGGACGGACTGACGCGGCCGC
GCTGGCTGATCGGCACGTTCGCCCGGACACTGCTCCGGCACGGGATGCCGCACTTCGAAAATTCGTTCGCGACGCGCGGC
GCGCCGATCCTGTCCGCGCATGTGCTGCGCGACTTCTCCGCGCGCGATCACCTCGACTGGACGCATCTCGCGCAGATTCG
TGCGCAATGGAAAGGCAATCTGGTCGTCAAGGGCATCCTCAGCGTGGACGATGCGCTGGCCGCACGCGACGTCGGCGCGG
ACGGCATCATCCTGTCCAATCATGGCGGCCGCCAACTCGATGGCACGGTGTCGCCGATGCGTATTCTGCGCGACGTCGTG
ACGGCTCTCGAGCCTGCGTTCCCGGTGATGCTCGACGGCGGCTTCCGACGCGGTGCCGACATTCTGAAGGCCATTGCGCT
GGGAGCGCGGATGGTGTTCGTCGGTCGACCGTTCAACTATGCAATGGCCGTTGCCGGCGAAGCGGGCGTAACGCACGCGA
TCCGGCTGTTGCAGGAAGAGGTCGATCGCGACATGGCGATGCTCGGGGCGCGTACTTGCCTCGATCTGCATCCTGGGCTG
ATCGTCCGCAAGCGCTGA

Upstream 100 bases:

>100_bases
TTCTCTTTTAGCGAACATGAATTTCGAAGGAATATCGCTTTTATTCAAACCGCGGTCAACCTAGCATGAGCCCTCGTCTT
GAATCAAAAGGAGAATCCGG

Downstream 100 bases:

>100_bases
GAGACGCATGCCGCACGGCGGTGCTCGAGCGATTGACGACGACGGCGCCAGAACGCCCTCACGTGCACATCGCCTACCCC
TTGCCGGTCGGTCGGCGTCG

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MRVVDRATLRRDAAAPTDKPPRVLRSMLSLHDFEARARRVLPRPIFGYVSGAAEDNRTRDDNRSVFDEFGFTTRVLRNVS
ARTQAVDLFGQRFAAPFGIAPMGINALSAYRGDIVLARAAQAAGIASIMSGSSLIPLEAVAAAAPSTWFQAYLPGDPERI
AALLERVARAGYRTLVVTVDIPVAANRENNVRTGFSTPLRPSLRLAWDGLTRPRWLIGTFARTLLRHGMPHFENSFATRG
APILSAHVLRDFSARDHLDWTHLAQIRAQWKGNLVVKGILSVDDALAARDVGADGIILSNHGGRQLDGTVSPMRILRDVV
TALEPAFPVMLDGGFRRGADILKAIALGARMVFVGRPFNYAMAVAGEAGVTHAIRLLQEEVDRDMAMLGARTCLDLHPGL
IVRKR

Sequences:

>Translated_405_residues
MRVVDRATLRRDAAAPTDKPPRVLRSMLSLHDFEARARRVLPRPIFGYVSGAAEDNRTRDDNRSVFDEFGFTTRVLRNVS
ARTQAVDLFGQRFAAPFGIAPMGINALSAYRGDIVLARAAQAAGIASIMSGSSLIPLEAVAAAAPSTWFQAYLPGDPERI
AALLERVARAGYRTLVVTVDIPVAANRENNVRTGFSTPLRPSLRLAWDGLTRPRWLIGTFARTLLRHGMPHFENSFATRG
APILSAHVLRDFSARDHLDWTHLAQIRAQWKGNLVVKGILSVDDALAARDVGADGIILSNHGGRQLDGTVSPMRILRDVV
TALEPAFPVMLDGGFRRGADILKAIALGARMVFVGRPFNYAMAVAGEAGVTHAIRLLQEEVDRDMAMLGARTCLDLHPGL
IVRKR
>Mature_405_residues
MRVVDRATLRRDAAAPTDKPPRVLRSMLSLHDFEARARRVLPRPIFGYVSGAAEDNRTRDDNRSVFDEFGFTTRVLRNVS
ARTQAVDLFGQRFAAPFGIAPMGINALSAYRGDIVLARAAQAAGIASIMSGSSLIPLEAVAAAAPSTWFQAYLPGDPERI
AALLERVARAGYRTLVVTVDIPVAANRENNVRTGFSTPLRPSLRLAWDGLTRPRWLIGTFARTLLRHGMPHFENSFATRG
APILSAHVLRDFSARDHLDWTHLAQIRAQWKGNLVVKGILSVDDALAARDVGADGIILSNHGGRQLDGTVSPMRILRDVV
TALEPAFPVMLDGGFRRGADILKAIALGARMVFVGRPFNYAMAVAGEAGVTHAIRLLQEEVDRDMAMLGARTCLDLHPGL
IVRKR

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI54234014, Length=378, Percent_Identity=34.9206349206349, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI7705393, Length=378, Percent_Identity=34.9206349206349, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI11068137, Length=372, Percent_Identity=33.0645161290323, Blast_Score=205, Evalue=7e-53,
Organism=Escherichia coli, GI1790033, Length=380, Percent_Identity=35.5263157894737, Blast_Score=211, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI193208036, Length=384, Percent_Identity=33.3333333333333, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI32566217, Length=305, Percent_Identity=30.1639344262295, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323587, Length=394, Percent_Identity=28.9340101522843, Blast_Score=173, Evalue=4e-44,
Organism=Drosophila melanogaster, GI78707188, Length=381, Percent_Identity=32.0209973753281, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI281363140, Length=381, Percent_Identity=32.0209973753281, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI78707190, Length=381, Percent_Identity=32.0209973753281, Blast_Score=185, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 44220; Mature: 44220

Theoretical pI: Translated: 11.37; Mature: 11.37

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVDRATLRRDAAAPTDKPPRVLRSMLSLHDFEARARRVLPRPIFGYVSGAAEDNRTRD
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
DNRSVFDEFGFTTRVLRNVSARTQAVDLFGQRFAAPFGIAPMGINALSAYRGDIVLARAA
CCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEEEHHH
QAAGIASIMSGSSLIPLEAVAAAAPSTWFQAYLPGDPERIAALLERVARAGYRTLVVTVD
HHHHHHHHHCCCCCCCHHHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEEE
IPVAANRENNVRTGFSTPLRPSLRLAWDGLTRPRWLIGTFARTLLRHGMPHFENSFATRG
CCEECCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCC
APILSAHVLRDFSARDHLDWTHLAQIRAQWKGNLVVKGILSVDDALAARDVGADGIILSN
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCEEEEC
HGGRQLDGTVSPMRILRDVVTALEPAFPVMLDGGFRRGADILKAIALGARMVFVGRPFNY
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCEEEEEECCCCE
AMAVAGEAGVTHAIRLLQEEVDRDMAMLGARTCLDLHPGLIVRKR
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MRVVDRATLRRDAAAPTDKPPRVLRSMLSLHDFEARARRVLPRPIFGYVSGAAEDNRTRD
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
DNRSVFDEFGFTTRVLRNVSARTQAVDLFGQRFAAPFGIAPMGINALSAYRGDIVLARAA
CCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEEEHHH
QAAGIASIMSGSSLIPLEAVAAAAPSTWFQAYLPGDPERIAALLERVARAGYRTLVVTVD
HHHHHHHHHCCCCCCCHHHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEEE
IPVAANRENNVRTGFSTPLRPSLRLAWDGLTRPRWLIGTFARTLLRHGMPHFENSFATRG
CCEECCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCC
APILSAHVLRDFSARDHLDWTHLAQIRAQWKGNLVVKGILSVDDALAARDVGADGIILSN
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCEEEEC
HGGRQLDGTVSPMRILRDVVTALEPAFPVMLDGGFRRGADILKAIALGARMVFVGRPFNY
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCEEEEEECCCCE
AMAVAGEAGVTHAIRLLQEEVDRDMAMLGARTCLDLHPGLIVRKR
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]