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The map label for this gene is cobB [H]

Identifier: 161524761

GI number: 161524761

Start: 1741433

End: 1742737

Strand: Direct

Name: cobB [H]

Synonym: Bmul_1588

Alternate gene names: 161524761

Gene position: 1741433-1742737 (Clockwise)

Preceding gene: 161524760

Following gene: 161524763

Centisome position: 50.5

GC content: 72.57

Gene sequence:

>1305_bases
ATGCCGGCCTGTCCCGCGCTGTTCGTCAGCGCACCGGCGTCCGGCCAGGGCAAGACCTCGGTGACGGCCGGTCTCGCGCG
GCTGCATCGGCGGCTCGGCCGCCGCGTGCGCGTGTTCAAGACGGGGCCGGATTTTCTCGATCCGATGCTGCTCGAACGCG
CGAGCGGCGCGCCCGTGCATGCGCTCGATCTCGGCATGGTCGGCGAGGCCGGCTGTCGCGCGCTGCTCGCCGACGCCGCG
CGCGACGCGGACCTGATCCTGATCGAAGGCGTGATGGGCCTGTTCGACGGCACGCCGAGCAGTGCCGATCTCGCGGCCGC
GTTCGGCGTGCCGATCGTCGCGGTGATCTCCGCGAAATCGGTCGCGCGGACGTTCGCGGCCATCGCGTTCGGACTCGCGC
GCTTCCGCCCCGGCCTGCCGTTTCACGGCGTGTTCGCGAACCGCGTCGGCTCCGACCGGCACGCGGCGCTGCTGCGGCAG
GCGCTGCCCGACGATCTGCGCTGGCTCGGGCATCTGCCGGCCGATGCGGCGCTGGCCTTGCCGGAACGGCATCTCGGGCT
GCACCAGCCGGACGATATCGCCGATCTCGACGCGCGGCTCGATCGCGCGGCCGATGCGCTCGCGCACACGGCGCTCGCCG
CGTTGCCGCCCGCGATCGGCTTCGCGCCGCCGGCCGTGGCCGCGCCGCTGCCGCGCACGCTCGCCGGCAAGCGCATCGCG
ATCGCGCGCGATGCCGCGTTCTCGTTCATCTACCCGGCCAATCTCGCGCTGCTCGACGCGCTCGGCGCGCAGGTGCGGTA
CTTTTCGCCGCTCGCCGACGAACCGGTGCCCGATGGATGCGACGCGCTGTTCCTGCCGGGCGGCTATCCGGAATTGCATG
CGCGTACGCTCGCCGCGAATCGGACGAGCGCGGCGACGATTCGCGCCCATGCGGCGGCCGGGCGGCCGATCGTCGCCGAA
TGCGGCGGAATGCTCTATCTATGCGAATCGCTGACCGACGTCGACGGCACGACGACGCCGATGCTCGGGCTGCTGCCCGG
CCATGCGACGATGCAGCGCCGCTTCGCGGCGCTCGGCATGCAGCAGCTCGACACGCGGGCCGGCACGCTTCGCGGCCACA
CGTTCCACTATTCGCGGCTCGCGACGCCGCTGGCGCCGGCCGCGATCGCGCAGCGCCCGGACGACGACGCGCGCAGCGGC
GAAGCCGTCTATCGCATCGGCGCGATCGTCGCAACCTACATGCACCTGTACTGGCCGTCGAATCCGCACGCGACGGCCGC
GCTGTTCACCGGCGACGCATTCTGA

Upstream 100 bases:

>100_bases
GCTGATCGACGCGGCCGACCTCGTCACCGAAATGCGGCTCGTCAAGCATCCGTACAAGGAGCAAGGCGTGAAGGCGCAGC
GCGGCGTGGAGTTCTGAGCG

Downstream 100 bases:

>100_bases
CGATGCGCGAGCCGGCGCCGGCGACGCCGGTGCGCGATTCGGCCGGCGCATCAGACGAGGAACGCCTCGGTCAGCTTGAC
CCAGTACGACGCGCCGGTCG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVHALDLGMVGEAGCRALLADAA
RDADLILIEGVMGLFDGTPSSADLAAAFGVPIVAVISAKSVARTFAAIAFGLARFRPGLPFHGVFANRVGSDRHAALLRQ
ALPDDLRWLGHLPADAALALPERHLGLHQPDDIADLDARLDRAADALAHTALAALPPAIGFAPPAVAAPLPRTLAGKRIA
IARDAAFSFIYPANLALLDALGAQVRYFSPLADEPVPDGCDALFLPGGYPELHARTLAANRTSAATIRAHAAAGRPIVAE
CGGMLYLCESLTDVDGTTTPMLGLLPGHATMQRRFAALGMQQLDTRAGTLRGHTFHYSRLATPLAPAAIAQRPDDDARSG
EAVYRIGAIVATYMHLYWPSNPHATAALFTGDAF

Sequences:

>Translated_434_residues
MPACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVHALDLGMVGEAGCRALLADAA
RDADLILIEGVMGLFDGTPSSADLAAAFGVPIVAVISAKSVARTFAAIAFGLARFRPGLPFHGVFANRVGSDRHAALLRQ
ALPDDLRWLGHLPADAALALPERHLGLHQPDDIADLDARLDRAADALAHTALAALPPAIGFAPPAVAAPLPRTLAGKRIA
IARDAAFSFIYPANLALLDALGAQVRYFSPLADEPVPDGCDALFLPGGYPELHARTLAANRTSAATIRAHAAAGRPIVAE
CGGMLYLCESLTDVDGTTTPMLGLLPGHATMQRRFAALGMQQLDTRAGTLRGHTFHYSRLATPLAPAAIAQRPDDDARSG
EAVYRIGAIVATYMHLYWPSNPHATAALFTGDAF
>Mature_433_residues
PACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVHALDLGMVGEAGCRALLADAAR
DADLILIEGVMGLFDGTPSSADLAAAFGVPIVAVISAKSVARTFAAIAFGLARFRPGLPFHGVFANRVGSDRHAALLRQA
LPDDLRWLGHLPADAALALPERHLGLHQPDDIADLDARLDRAADALAHTALAALPPAIGFAPPAVAAPLPRTLAGKRIAI
ARDAAFSFIYPANLALLDALGAQVRYFSPLADEPVPDGCDALFLPGGYPELHARTLAANRTSAATIRAHAAAGRPIVAEC
GGMLYLCESLTDVDGTTTPMLGLLPGHATMQRRFAALGMQQLDTRAGTLRGHTFHYSRLATPLAPAAIAQRPDDDARSGE
AVYRIGAIVATYMHLYWPSNPHATAALFTGDAF

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 45482; Mature: 45351

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVH
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEE
ALDLGMVGEAGCRALLADAARDADLILIEGVMGLFDGTPSSADLAAAFGVPIVAVISAKS
EEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHH
VARTFAAIAFGLARFRPGLPFHGVFANRVGSDRHAALLRQALPDDLRWLGHLPADAALAL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHC
PERHLGLHQPDDIADLDARLDRAADALAHTALAALPPAIGFAPPAVAAPLPRTLAGKRIA
CHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHCCCEEE
IARDAAFSFIYPANLALLDALGAQVRYFSPLADEPVPDGCDALFLPGGYPELHARTLAAN
EEECCCEEEECCCHHHHHHHHCCHHEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCC
RTSAATIRAHAAAGRPIVAECGGMLYLCESLTDVDGTTTPMLGLLPGHATMQRRFAALGM
CCCHHEEEEHHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHH
QQLDTRAGTLRGHTFHYSRLATPLAPAAIAQRPDDDARSGEAVYRIGAIVATYMHLYWPS
HHHHHHHCCCCCCEEEHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
NPHATAALFTGDAF
CCCCEEEEEECCCC
>Mature Secondary Structure 
PACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVH
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEE
ALDLGMVGEAGCRALLADAARDADLILIEGVMGLFDGTPSSADLAAAFGVPIVAVISAKS
EEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHH
VARTFAAIAFGLARFRPGLPFHGVFANRVGSDRHAALLRQALPDDLRWLGHLPADAALAL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHC
PERHLGLHQPDDIADLDARLDRAADALAHTALAALPPAIGFAPPAVAAPLPRTLAGKRIA
CHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHCCCEEE
IARDAAFSFIYPANLALLDALGAQVRYFSPLADEPVPDGCDALFLPGGYPELHARTLAAN
EEECCCEEEECCCHHHHHHHHCCHHEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCC
RTSAATIRAHAAAGRPIVAECGGMLYLCESLTDVDGTTTPMLGLLPGHATMQRRFAALGM
CCCHHEEEEHHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHH
QQLDTRAGTLRGHTFHYSRLATPLAPAAIAQRPDDDARSGEAVYRIGAIVATYMHLYWPS
HHHHHHHCCCCCCEEEHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
NPHATAALFTGDAF
CCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA