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The map label for this gene is nifA [H]

Identifier: 161524689

GI number: 161524689

Start: 1668826

End: 1670214

Strand: Direct

Name: nifA [H]

Synonym: Bmul_1516

Alternate gene names: 161524689

Gene position: 1668826-1670214 (Clockwise)

Preceding gene: 161524685

Following gene: 161524694

Centisome position: 48.39

GC content: 70.63

Gene sequence:

>1389_bases
ATGAATCCGCACGACACCATTCCGATCGCTCCCGTGCCGCCGCACGAACCGGCGCTCGCGCCCGACGTGCGGGCGCTCGT
CGCCTATCTCGAACAGGATCCGCAGCCGATGATCGTCGTCGATCCCGACTACCGGATCCTCGCGGCGAACGGCGCGTACC
GGCGGCAGTTCGGCGTCGCGGGCGTCGAGCACGTCGGCCGGCACTGTTTTCAGGTATCGCACCATTACGACGTGCCGTGC
GACCAGGCCGGCGAGCACTGCCCGATGAAGCGCGCAAAGGCGCTGCGCGGCCTCGATCGCGTGCTGCATATTCATCACAC
GCCGCGCGGGCCCGAGCACGTCGACGTCGAGCTGCGGCCGATCTTCGACGCGGGCGGCGACGTGATCGCGTATGTCGAGC
GGCTGACGACCGTGCGCAGCGCGTCGGCGCAGCCGAGCGCGGAAGGGCTCGTCGGCAGCGCGGAGTCGTTCAATGCGGCG
TTGGGCGCGCTGCAGCGCGTCGCCCCGTCGACGCTGCCGGTGCTGCTGCTCGGCGAGTCGGGCACCGGCAAGGAATTGTT
CGCGCGTGCGCTGCACGAGGCGAGCGATCGGGCGATGGGGCCGTTCGTCGTCGTCGATTGTTCGGGGATTGCCGAAACGC
TGTTCGAGAGCGAACTGTTCGGCTACGAGAAGGGGGCATTTACGGGCGCGAACCAGCGCAAGCCGGGCCTCGTCGAGACG
GCGCAGGGCGGCACGCTGTTTCTCGACGAGATCGGCGACGTGCCGCTGCCGATGCAGGTGAAGCTGCTGCGGCTGATCGA
ATCGGGCACGTTCCGGCGCGTCGGCGGCGTCGAGGCGCTGCGCGCCGATTTCCGGCTCGTCGCGGCGACGCACAAGCCGC
TGCGCGACATGATCGCCGACGGCCGCTTTCGGCAGGATCTGTACTACCGGATCAATGCGTTTCCGATCGCGCTGCCGCCG
TTGCGCGAGCGGCGCGGCGACGTCGCGCTGCTTGCCGAGTCGATCCTGCGACGGATCGCGAACGCGCGCGCGGCTGCCGG
CGACGCGCTCGCGCGCCCGTACGTGCTGACCGAACCGGCGCTCGCGTGTCTCGATGCGTATGCGTGGCCCGGCAACATCC
GCGAGCTGCGCAACGTGCTCGAACGCGCGTGCCTGTTCGCGGACGACGGCACGATCCGCGTCGAGCATCTGCCGGCCGAA
CTCGTGTCGGCCGCCGCTGCGCCGCCGCCTGTGGCGGCGGCCGCGCGCGGCGTATCCGACGCGGAACTCGTGCGGCTCGC
CGCGTCGTTCGGCGGCACGCGCAAGGCGCTGGCCGCGTACGTCGGCCTCAGCGAGCGCACGCTTTACCGGCGAATGAAGG
CGCTCGGGTTACGCGTGCGCACGCGCTGA

Upstream 100 bases:

>100_bases
CAGAATTGGCAGTCTTTGTGGCAGTCCTTGTGGCAGTTCCGGCCGAGCGCGCTGGCTCGTCGCGGCCGCCGCATCCTCTG
ATCGAACCCCCGGAATCCCG

Downstream 100 bases:

>100_bases
ACCGCGCGTGCGTTGCATTGCGCGACGCGCCGCACGGCGCACGGCGCGCGAGACGCGGCACACGGCAGACGGCGCGCGGC
GCATGACACACGGCGCACGA

Product: PAS modulated sigma54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MNPHDTIPIAPVPPHEPALAPDVRALVAYLEQDPQPMIVVDPDYRILAANGAYRRQFGVAGVEHVGRHCFQVSHHYDVPC
DQAGEHCPMKRAKALRGLDRVLHIHHTPRGPEHVDVELRPIFDAGGDVIAYVERLTTVRSASAQPSAEGLVGSAESFNAA
LGALQRVAPSTLPVLLLGESGTGKELFARALHEASDRAMGPFVVVDCSGIAETLFESELFGYEKGAFTGANQRKPGLVET
AQGGTLFLDEIGDVPLPMQVKLLRLIESGTFRRVGGVEALRADFRLVAATHKPLRDMIADGRFRQDLYYRINAFPIALPP
LRERRGDVALLAESILRRIANARAAAGDALARPYVLTEPALACLDAYAWPGNIRELRNVLERACLFADDGTIRVEHLPAE
LVSAAAAPPPVAAAARGVSDAELVRLAASFGGTRKALAAYVGLSERTLYRRMKALGLRVRTR

Sequences:

>Translated_462_residues
MNPHDTIPIAPVPPHEPALAPDVRALVAYLEQDPQPMIVVDPDYRILAANGAYRRQFGVAGVEHVGRHCFQVSHHYDVPC
DQAGEHCPMKRAKALRGLDRVLHIHHTPRGPEHVDVELRPIFDAGGDVIAYVERLTTVRSASAQPSAEGLVGSAESFNAA
LGALQRVAPSTLPVLLLGESGTGKELFARALHEASDRAMGPFVVVDCSGIAETLFESELFGYEKGAFTGANQRKPGLVET
AQGGTLFLDEIGDVPLPMQVKLLRLIESGTFRRVGGVEALRADFRLVAATHKPLRDMIADGRFRQDLYYRINAFPIALPP
LRERRGDVALLAESILRRIANARAAAGDALARPYVLTEPALACLDAYAWPGNIRELRNVLERACLFADDGTIRVEHLPAE
LVSAAAAPPPVAAAARGVSDAELVRLAASFGGTRKALAAYVGLSERTLYRRMKALGLRVRTR
>Mature_462_residues
MNPHDTIPIAPVPPHEPALAPDVRALVAYLEQDPQPMIVVDPDYRILAANGAYRRQFGVAGVEHVGRHCFQVSHHYDVPC
DQAGEHCPMKRAKALRGLDRVLHIHHTPRGPEHVDVELRPIFDAGGDVIAYVERLTTVRSASAQPSAEGLVGSAESFNAA
LGALQRVAPSTLPVLLLGESGTGKELFARALHEASDRAMGPFVVVDCSGIAETLFESELFGYEKGAFTGANQRKPGLVET
AQGGTLFLDEIGDVPLPMQVKLLRLIESGTFRRVGGVEALRADFRLVAATHKPLRDMIADGRFRQDLYYRINAFPIALPP
LRERRGDVALLAESILRRIANARAAAGDALARPYVLTEPALACLDAYAWPGNIRELRNVLERACLFADDGTIRVEHLPAE
LVSAAAAPPPVAAAARGVSDAELVRLAASFGGTRKALAAYVGLSERTLYRRMKALGLRVRTR

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=303, Percent_Identity=40.9240924092409, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=43.4083601286174, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789087, Length=336, Percent_Identity=40.4761904761905, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1789233, Length=337, Percent_Identity=38.8724035608309, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1790299, Length=288, Percent_Identity=42.0138888888889, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082117, Length=327, Percent_Identity=38.8379204892966, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1786524, Length=302, Percent_Identity=42.7152317880795, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=239, Percent_Identity=46.4435146443515, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=40.2476780185758, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1788905, Length=220, Percent_Identity=45, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1787583, Length=304, Percent_Identity=36.5131578947368, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI87081858, Length=299, Percent_Identity=33.4448160535117, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789828, Length=306, Percent_Identity=34.9673202614379, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50024; Mature: 50024

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPHDTIPIAPVPPHEPALAPDVRALVAYLEQDPQPMIVVDPDYRILAANGAYRRQFGVA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHCCC
GVEHVGRHCFQVSHHYDVPCDQAGEHCPMKRAKALRGLDRVLHIHHTPRGPEHVDVELRP
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEEE
IFDAGGDVIAYVERLTTVRSASAQPSAEGLVGSAESFNAALGALQRVAPSTLPVLLLGES
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC
GTGKELFARALHEASDRAMGPFVVVDCSGIAETLFESELFGYEKGAFTGANQRKPGLVET
CCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
AQGGTLFLDEIGDVPLPMQVKLLRLIESGTFRRVGGVEALRADFRLVAATHKPLRDMIAD
CCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHEEHHHHHHHHHHHC
GRFRQDLYYRINAFPIALPPLRERRGDVALLAESILRRIANARAAAGDALARPYVLTEPA
CCHHHHHEEEEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCEEECCHH
LACLDAYAWPGNIRELRNVLERACLFADDGTIRVEHLPAELVSAAAAPPPVAAAARGVSD
HHHHHHHCCCCCHHHHHHHHHHHHEECCCCEEEEEECCHHHHHHCCCCCCHHHHHCCCCH
AELVRLAASFGGTRKALAAYVGLSERTLYRRMKALGLRVRTR
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCEECCC
>Mature Secondary Structure
MNPHDTIPIAPVPPHEPALAPDVRALVAYLEQDPQPMIVVDPDYRILAANGAYRRQFGVA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHCCC
GVEHVGRHCFQVSHHYDVPCDQAGEHCPMKRAKALRGLDRVLHIHHTPRGPEHVDVELRP
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEEE
IFDAGGDVIAYVERLTTVRSASAQPSAEGLVGSAESFNAALGALQRVAPSTLPVLLLGES
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC
GTGKELFARALHEASDRAMGPFVVVDCSGIAETLFESELFGYEKGAFTGANQRKPGLVET
CCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
AQGGTLFLDEIGDVPLPMQVKLLRLIESGTFRRVGGVEALRADFRLVAATHKPLRDMIAD
CCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHEEHHHHHHHHHHHC
GRFRQDLYYRINAFPIALPPLRERRGDVALLAESILRRIANARAAAGDALARPYVLTEPA
CCHHHHHEEEEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCEEECCHH
LACLDAYAWPGNIRELRNVLERACLFADDGTIRVEHLPAELVSAAAAPPPVAAAARGVSD
HHHHHHHCCCCCHHHHHHHHHHHHEECCCCEEEEEECCHHHHHHCCCCCCHHHHHCCCCH
AELVRLAASFGGTRKALAAYVGLSERTLYRRMKALGLRVRTR
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840552; 2450865 [H]