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The map label for this gene is pit [H]

Identifier: 161524579

GI number: 161524579

Start: 1547294

End: 1548304

Strand: Direct

Name: pit [H]

Synonym: Bmul_1406

Alternate gene names: 161524579

Gene position: 1547294-1548304 (Clockwise)

Preceding gene: 161524578

Following gene: 161524591

Centisome position: 44.87

GC content: 64.59

Gene sequence:

>1011_bases
ATGCATTCGATACAACTCGCCCTATGGATGGTCGCGACGCTGGTGCTCGTCGCGCTCGTATTCGACTTCATGAACGGCTT
CCACGACGCGGCGAACTCGATCGCCACCGTCGTGTCGACCGGGGTGCTGAAGCCGCAGCAGGCCGTCGTGTTCGCGGCGG
CCTTTAACGTGATCGCGTATTTCATCTTCCACCTGAAGGTCGCGCAGACCGTCGGCAAAGGCACGATCGATCCGGAGATC
GTCGATCACTACGTCGTGTTCGGCGCGCTCGTCGGTGCGATCGGCTGGAACGTGATCACGTGGTACTACGGGATTCCGTC
GAGCTCGTCGCACGCGCTGATCGGCGGGCTGGTCGGCTCCGCACTCGCGAAATCGGGCTGGAGCGCACTGAATTTGGACG
GGCTGATGAAGACCGTCGCGTTCATCTTCATTTCGCCGCTGCTCGGCTTCATTCTCGGCTCGCTGTTCATGCTCGGCGTG
TCGTGGCTGTATTTCCGTACCGCACCGAGCAAGGTCGACCGGCGCTTCCGCCGGCTTCAACTGCTGTCGGCCGGGCTCTA
TAGCCTCGGTCACGGCGGCAACGACGCGCAGAAGACGATCGGCATCATCTGGATGCTGCTGATCGCGTCCGGCTACGCGT
CGGCGACGTCCGATGCGCCGCCTGCCTGGGTGATCGGCGCCTGCTATCTGTCGATGGGCCTCGGCACGCTGTTCGGCGGC
TGGCGCATCGTGCGCACGATGGGCCAGAAGATCACGAAGCTGAAGCCGGTCGGCGGCTTCTGTGCGGAAAGCGGCGGTGC
GATCACGCTGTTCGTCGCGTCGTTCCTCGGCATTCCGGTATCGACGACGCACACGATCACCGGCGCGATCGTCGGTGTCG
GCGCGACGCAGAAGCTGTCGGCCGTGCGCTGGGGTGTTGCCGGCAACATCGTGTGGGCGTGGGTGCTGACGATTCCCGCA
TCCGCGCTGATCGCCGCCGGCGGCTGGTGGCTCGGGCACCGGATCTTCTGA

Upstream 100 bases:

>100_bases
AAGGCGATCTACGAACTGCTCGAGGAGATCACCGACAAGTGCGAGGACGTCGCGAACATCATCGAAGGCATCGTGCTGGA
AAACGCCTGAGCGGATCACG

Downstream 100 bases:

>100_bases
TTCCGGCGCGCGATCCAGTCGCCGCTTCGAGAACAGGGCGCCGCATGCCGAACAGGCTGCGGCGCCTTTTTTTGCGTGCG
GACCACGCGTGTGTGGGAAG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MHSIQLALWMVATLVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIFHLKVAQTVGKGTIDPEI
VDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGSALAKSGWSALNLDGLMKTVAFIFISPLLGFILGSLFMLGV
SWLYFRTAPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATSDAPPAWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAESGGAITLFVASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTIPA
SALIAAGGWWLGHRIF

Sequences:

>Translated_336_residues
MHSIQLALWMVATLVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIFHLKVAQTVGKGTIDPEI
VDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGSALAKSGWSALNLDGLMKTVAFIFISPLLGFILGSLFMLGV
SWLYFRTAPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATSDAPPAWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAESGGAITLFVASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTIPA
SALIAAGGWWLGHRIF
>Mature_336_residues
MHSIQLALWMVATLVLVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVVFAAAFNVIAYFIFHLKVAQTVGKGTIDPEI
VDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGSALAKSGWSALNLDGLMKTVAFIFISPLLGFILGSLFMLGV
SWLYFRTAPSKVDRRFRRLQLLSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASATSDAPPAWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAESGGAITLFVASFLGIPVSTTHTITGAIVGVGATQKLSAVRWGVAGNIVWAWVLTIPA
SALIAAGGWWLGHRIF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=168, Percent_Identity=36.3095238095238, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI5803173, Length=156, Percent_Identity=36.5384615384615, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1789360, Length=255, Percent_Identity=32.5490196078431, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=31.3364055299539, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17539280, Length=176, Percent_Identity=32.3863636363636, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=163, Percent_Identity=31.9018404907975, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71981576, Length=163, Percent_Identity=28.8343558282209, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566716, Length=169, Percent_Identity=30.1775147928994, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17536725, Length=169, Percent_Identity=31.3609467455621, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557328, Length=155, Percent_Identity=33.5483870967742, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319773, Length=172, Percent_Identity=31.9767441860465, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356511, Length=182, Percent_Identity=31.3186813186813, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35592; Mature: 35592

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure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HHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]