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The map label for this gene is aes [H]

Identifier: 161524549

GI number: 161524549

Start: 1520363

End: 1521322

Strand: Direct

Name: aes [H]

Synonym: Bmul_1376

Alternate gene names: 161524549

Gene position: 1520363-1521322 (Clockwise)

Preceding gene: 161524548

Following gene: 161524550

Centisome position: 44.09

GC content: 70.83

Gene sequence:

>960_bases
ATGCCGCTGAATCCGAAGATCGCCCAGGTGCTCGACATGGTCGAGCGCGCGAAACGCCCGCCATATCATCGCCAGACGCC
GCAGCAGGCGCGCGCGGCCTACGAGAAGAGCGCGCCGATCCTCGACGTGCCGCCCGCGCCGATGCATACGGTCGACGCGT
GCACGATTCCGACGCGCGACGGCCATGCGATTGCCGCGCGGCTGTATCTGCCGGTCGAACCTAGCCTCGCCGAGCCGCTG
CCCGCGCTCGTCTACTATCACGGCGGCGGCTTCACGGTCGGCAGCATCGACACGCACGACGCGCTGTGCCGGATGTTCGC
GCGCGACGCGCGCTGCGCGGTGCTGTCGGTCGGCTACCGCCTCGCGCCCGAGCACCGGTTCCCGACCGCCGTCAACGATG
CCGAGGACGCATTGCGCTGGCTGCATCGCGAGGCGCCCGCATTGGGCCTCGACGCGTCGCGGCTCGCGGTCGGCGGCGAC
AGCGCGGGCGGCACGCTCGCGACGGTCTGCGCGGTGCTCGCACGCGATGCGGGCATCGATCTTGCGCTACAGCTGCTGAT
TTACCCCGGCGTCACGGGCCATCAGGACACCGCCTCGCATGCGCGGCTCGCGAACGGCTACCTGCTGTCGCGCGACACGA
TCCAGTGGTTCTTCGCGCAATACGTGCGCGATGCGGCGGATCGCGACGACTGGCGCTTCGCGCCGCTCGACGGCCGGCGC
GGCGCGCCGTCGTTCGCGGGCGTCGCGCCCGCGTGGATCGCGACGGCCGAGTACGACCCGCTCAGCGACGAAGGCGCCGC
GTATGCGGACAAGCTGCGCGCGGCCGGCAACACGGTCACGCTGGTCTGCTATCCGGGCATGATCCACGAATTCTTCAAGA
TGGGCGGCTACGTGCCCGAGGTGAAGGCCGCGCATGCCGATGCGGTCGCGGCGCTGAGGGCCGCGTTCGACGACGTTTGA

Upstream 100 bases:

>100_bases
GCCGCAATTTCGGCAAGCAGCTCGTCCAGCTCGTGTGACGCAGGCGTGGCATCATGTGGCGCTTGTTCGTTCCCGGCGCC
GCGCCCGCGCCGTTCACCCG

Downstream 100 bases:

>100_bases
CGGCGGGCGGCGACGGCGCCGCGCGCGAGGAGGGCAGCGATGACGGACGGCGTGGTGGAAACCGGCGACTGGACGACGCT
GGGCGGCGATGCGGCGCGCA

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MPLNPKIAQVLDMVERAKRPPYHRQTPQQARAAYEKSAPILDVPPAPMHTVDACTIPTRDGHAIAARLYLPVEPSLAEPL
PALVYYHGGGFTVGSIDTHDALCRMFARDARCAVLSVGYRLAPEHRFPTAVNDAEDALRWLHREAPALGLDASRLAVGGD
SAGGTLATVCAVLARDAGIDLALQLLIYPGVTGHQDTASHARLANGYLLSRDTIQWFFAQYVRDAADRDDWRFAPLDGRR
GAPSFAGVAPAWIATAEYDPLSDEGAAYADKLRAAGNTVTLVCYPGMIHEFFKMGGYVPEVKAAHADAVAALRAAFDDV

Sequences:

>Translated_319_residues
MPLNPKIAQVLDMVERAKRPPYHRQTPQQARAAYEKSAPILDVPPAPMHTVDACTIPTRDGHAIAARLYLPVEPSLAEPL
PALVYYHGGGFTVGSIDTHDALCRMFARDARCAVLSVGYRLAPEHRFPTAVNDAEDALRWLHREAPALGLDASRLAVGGD
SAGGTLATVCAVLARDAGIDLALQLLIYPGVTGHQDTASHARLANGYLLSRDTIQWFFAQYVRDAADRDDWRFAPLDGRR
GAPSFAGVAPAWIATAEYDPLSDEGAAYADKLRAAGNTVTLVCYPGMIHEFFKMGGYVPEVKAAHADAVAALRAAFDDV
>Mature_318_residues
PLNPKIAQVLDMVERAKRPPYHRQTPQQARAAYEKSAPILDVPPAPMHTVDACTIPTRDGHAIAARLYLPVEPSLAEPLP
ALVYYHGGGFTVGSIDTHDALCRMFARDARCAVLSVGYRLAPEHRFPTAVNDAEDALRWLHREAPALGLDASRLAVGGDS
AGGTLATVCAVLARDAGIDLALQLLIYPGVTGHQDTASHARLANGYLLSRDTIQWFFAQYVRDAADRDDWRFAPLDGRRG
APSFAGVAPAWIATAEYDPLSDEGAAYADKLRAAGNTVTLVCYPGMIHEFFKMGGYVPEVKAAHADAVAALRAAFDDV

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=303, Percent_Identity=29.7029702970297, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI206597554, Length=172, Percent_Identity=36.046511627907, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI157041239, Length=143, Percent_Identity=36.3636363636364, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI61966717, Length=195, Percent_Identity=30.2564102564103, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI68051721, Length=272, Percent_Identity=27.5735294117647, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI226423947, Length=280, Percent_Identity=26.4285714285714, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=251, Percent_Identity=32.6693227091633, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71996133, Length=165, Percent_Identity=36.3636363636364, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=29.4339622641509, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=148, Percent_Identity=35.1351351351351, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=122, Percent_Identity=37.7049180327869, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24656084, Length=102, Percent_Identity=37.2549019607843, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24656076, Length=102, Percent_Identity=37.2549019607843, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20130169, Length=102, Percent_Identity=37.2549019607843, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34368; Mature: 34237

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNPKIAQVLDMVERAKRPPYHRQTPQQARAAYEKSAPILDVPPAPMHTVDACTIPTRD
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCCC
GHAIAARLYLPVEPSLAEPLPALVYYHGGGFTVGSIDTHDALCRMFARDARCAVLSVGYR
CCEEEEEEEECCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHCCCEEEEECCCE
LAPEHRFPTAVNDAEDALRWLHREAPALGLDASRLAVGGDSAGGTLATVCAVLARDAGID
ECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCHHHHHHHHHHHCCCHH
LALQLLIYPGVTGHQDTASHARLANGYLLSRDTIQWFFAQYVRDAADRDDWRFAPLDGRR
HEEHHHHCCCCCCCCCCHHHHHHHCCEEECHHHHHHHHHHHHHHHCCCCCCEECCCCCCC
GAPSFAGVAPAWIATAEYDPLSDEGAAYADKLRAAGNTVTLVCYPGMIHEFFKMGGYVPE
CCCCCCCCCHHHEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCC
VKAAHADAVAALRAAFDDV
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLNPKIAQVLDMVERAKRPPYHRQTPQQARAAYEKSAPILDVPPAPMHTVDACTIPTRD
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCCC
GHAIAARLYLPVEPSLAEPLPALVYYHGGGFTVGSIDTHDALCRMFARDARCAVLSVGYR
CCEEEEEEEECCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHCCCEEEEECCCE
LAPEHRFPTAVNDAEDALRWLHREAPALGLDASRLAVGGDSAGGTLATVCAVLARDAGID
ECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCHHHHHHHHHHHCCCHH
LALQLLIYPGVTGHQDTASHARLANGYLLSRDTIQWFFAQYVRDAADRDDWRFAPLDGRR
HEEHHHHCCCCCCCCCCHHHHHHHCCEEECHHHHHHHHHHHHHHHCCCCCCEECCCCCCC
GAPSFAGVAPAWIATAEYDPLSDEGAAYADKLRAAGNTVTLVCYPGMIHEFFKMGGYVPE
CCCCCCCCCHHHEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCC
VKAAHADAVAALRAAFDDV
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA