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The map label for this gene is pncA [H]

Identifier: 161524542

GI number: 161524542

Start: 1511843

End: 1512475

Strand: Direct

Name: pncA [H]

Synonym: Bmul_1369

Alternate gene names: 161524542

Gene position: 1511843-1512475 (Clockwise)

Preceding gene: 161524541

Following gene: 161524543

Centisome position: 43.84

GC content: 70.14

Gene sequence:

>633_bases
ATGAAACGCACCGACGACGTCCTGCTCGTGATCGACGTGCAGTACGACTTCATGCCGGGCGGCGCGCTGGCCGTCGCCGA
CGGCGACGCGGTCGTGCCCGTGATCAACGCGCTCGCGCAGCGGTTCGAGCGCGTCGTGATCACGCAGGACTGGCATCCGC
GCGAGCACGTGTCGTTCGCGGCGAATCATCCGGACGGCGAACCGTTCTCGACGATCGCGCTGCCGTACGGCGAGCAGGTG
CTGTGGCCCGTGCACTGCGTGCAGGACACCGAAGGCGCGGCGCTGCATCGCGACCTCGACCTGCCGCACGCGCAGCTCGT
GATTCGCAAGGGCTGCGATGCGCGCGTCGACAGCTATTCCGCATTCGTCGAAGCCGATCGCACGACGCGCACGGGGCTCG
CCGGCTATCTGCGCGAGATCGGCGCGAAACGCGTCTGGTGCTGCGGGCTCGCGACCGACTATTGCGTCGCGTGGTCGGCG
CTCGACGCGCGCGCGGCGGGCTTCGACGCCGCGGTGATCGACGATGCGTGCCGCGCGATCGACCTGAACGGCTCGCTCGC
GCAGGCATGGCAGCAGATGCAGGCGGCGGGCGTCGCACGCGTGACGTCCGATGGCGTGCGCGCCAGCGCATAG

Upstream 100 bases:

>100_bases
GGCAGCTCGCGCCGTCGATCGCGGAGCTCGCGAAGGCCGGCCGCGGATTCAACGGCTGACGCCACGCGGCGCCGCGCACC
GACTCGCAAGGAGAAACGCA

Downstream 100 bases:

>100_bases
CGCCGCCTTTCCACTCGATTCAGCACATCAGGCAGGAACGAAGGAGACTCGCATGACCGAAGCCGTAATCGTATCGACCG
CACGCACGCCGCTCGCGAAA

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKRTDDVLLVIDVQYDFMPGGALAVADGDAVVPVINALAQRFERVVITQDWHPREHVSFAANHPDGEPFSTIALPYGEQV
LWPVHCVQDTEGAALHRDLDLPHAQLVIRKGCDARVDSYSAFVEADRTTRTGLAGYLREIGAKRVWCCGLATDYCVAWSA
LDARAAGFDAAVIDDACRAIDLNGSLAQAWQQMQAAGVARVTSDGVRASA

Sequences:

>Translated_210_residues
MKRTDDVLLVIDVQYDFMPGGALAVADGDAVVPVINALAQRFERVVITQDWHPREHVSFAANHPDGEPFSTIALPYGEQV
LWPVHCVQDTEGAALHRDLDLPHAQLVIRKGCDARVDSYSAFVEADRTTRTGLAGYLREIGAKRVWCCGLATDYCVAWSA
LDARAAGFDAAVIDDACRAIDLNGSLAQAWQQMQAAGVARVTSDGVRASA
>Mature_210_residues
MKRTDDVLLVIDVQYDFMPGGALAVADGDAVVPVINALAQRFERVVITQDWHPREHVSFAANHPDGEPFSTIALPYGEQV
LWPVHCVQDTEGAALHRDLDLPHAQLVIRKGCDARVDSYSAFVEADRTTRTGLAGYLREIGAKRVWCCGLATDYCVAWSA
LDARAAGFDAAVIDDACRAIDLNGSLAQAWQQMQAAGVARVTSDGVRASA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=200, Percent_Identity=43, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI193206735, Length=225, Percent_Identity=32.4444444444444, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17543130, Length=225, Percent_Identity=32.4444444444444, Blast_Score=104, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71999339, Length=216, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=192, Percent_Identity=35.4166666666667, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648247, Length=232, Percent_Identity=34.051724137931, Blast_Score=123, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22708; Mature: 22708

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTDDVLLVIDVQYDFMPGGALAVADGDAVVPVINALAQRFERVVITQDWHPREHVSFA
CCCCCCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCHHHCEEE
ANHPDGEPFSTIALPYGEQVLWPVHCVQDTEGAALHRDLDLPHAQLVIRKGCDARVDSYS
CCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHCCCCCCCCCHH
AFVEADRTTRTGLAGYLREIGAKRVWCCGLATDYCVAWSALDARAAGFDAAVIDDACRAI
HHEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
DLNGSLAQAWQQMQAAGVARVTSDGVRASA
ECCCHHHHHHHHHHHHCHHEECCCCCCCCC
>Mature Secondary Structure
MKRTDDVLLVIDVQYDFMPGGALAVADGDAVVPVINALAQRFERVVITQDWHPREHVSFA
CCCCCCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCHHHCEEE
ANHPDGEPFSTIALPYGEQVLWPVHCVQDTEGAALHRDLDLPHAQLVIRKGCDARVDSYS
CCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHCCCCCCCCCHH
AFVEADRTTRTGLAGYLREIGAKRVWCCGLATDYCVAWSALDARAAGFDAAVIDDACRAI
HHEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
DLNGSLAQAWQQMQAAGVARVTSDGVRASA
ECCCHHHHHHHHHHHHCHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]