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The map label for this gene is pucI [H]

Identifier: 161524478

GI number: 161524478

Start: 1433725

End: 1435209

Strand: Direct

Name: pucI [H]

Synonym: Bmul_1305

Alternate gene names: 161524478

Gene position: 1433725-1435209 (Clockwise)

Preceding gene: 161524477

Following gene: 161524479

Centisome position: 41.58

GC content: 64.78

Gene sequence:

>1485_bases
ATGGCTCAGTTCAGTGTGGCGCACGACAGCGCCTATCCGGCGCAAGATGGCGGCGAGGGCGGCGGCCCCGCGTTGCCGGG
CGGTTATAGCGAGCGTCTGTACAACGAAGATTTGGCACCCCTGAAGAATCAGACCTGGGGTTCGTACAACATCTTTGCAT
TCTGGATGTCCGACGTGCACAGCGTCGGCGGCTACGTGTTCGCGGGCAGCCTGTTCGCGCTCGGGCTGACGAGCTGGCAG
GTGCTGATCGCGTTGATCGTCGGCATCAGCCTCGTCAACGTGCTGTGCAACCTGATCGCGCGGCCGAGCCAGCAGATCGG
CGTGCCGTATCCCGTCGCGTGCCGCGCGACGTTCGGTGTGCTCGGCGCGAACGTGCCGGCCGTGATTCGCGGGCTGATCG
CGATCGCGTGGTACGGGATCCAGACGTATCTCGCGTCGAGCGCGCTCGTGATCGTCGTGCTGAAGTTCTTCCCGCAGTGG
TTGCCGTATGCGGACGTTCATCGCTACGGCTTCCTCGGCCTGTCGGCCGTCGGCTGGGCCGGCTTCATGCTGCTGTGGGT
GCTGCAGGCGCTGGTGTTCTGGAACGGGATGGAGACGATCAAGAAGTTCATCGACTTCGCGGGCCCGGCCGTCTACGTCG
TGATGTTCATCCTCGCCGGCTACATGGTGTGGCGCGCGGGCTGGCGCAATATCGGGATCAACCTCGGCGGCGTCAAGTAT
CACGGCGCGGAAGTGATCCCGGTGATGATCACGGCCATCTCGCTCGTCGTGTCGTACTTCTCCGGGCCGATGCTGAATTT
CGGCGACTTCTCGCGGTACTGCCGCTCGTTCGGCGAAGTGAAGCGCGGCAACTTCTGGGGGCTGCCCGTGAACTTCCTCG
CGTTCTCGCTCGTCACCGTCATCACGACGGCCGCGACGCTGCCGGTGTTCGGCGAGCTGATCACCGATCCGGTCGAGACG
GTCGGCCGCATCGATCATCCGACCGCCGTGATTCTCGGCGCGCTGACCTTCACGATCGCGACGATCGGCATCAACATCGT
CGCAAACTTCGTGTCGCCGGCATTCGACTTCTCGAACGTCGCGCCGCGGCTGATCAGCTGGCGCGCGGGCGGGATGCTCG
CGGCGGTCGCCTCGGTGTTCATCACGCCGTGGAATCTCTTCAACAATCCGGCCGTGATCCATTACACGCTCGACGTGCTC
GGCGCATTCATCGGCCCGCTGTACGGCGTGCTGATCGCCGACTTCTATCTCGTCAAGCGCGGCCGGCTCGTGCGCGACGA
TCTCTACACGATGTCGGAGAGCGGCACTTACTGGTACACGGGCGGCGTGAACCGCCGCGCACTCGCCGCACTGCTGCCGG
CCGCGGTGATCGCGATCGTCTGCGTGATGATGCCGGGCTGGCAGGGCGTTGCGAACTTCTCGTGGTTCATCGGCGCGGCA
CTCGGCTTCGTGTTCTACCGCGTGCTCGCCGGCACGCAGCGTTGA

Upstream 100 bases:

>100_bases
CCGCGGCTCGCGCCGCGGGCCGCGGGTCCGACTCCGGACGACGATCCGGGGCGTTATCGATGACGGTGGAGCGGCCATCC
GGCCGCGTCAAGGAGAAGTC

Downstream 100 bases:

>100_bases
GGAGCGCGCCATGAAGATCAAGCTGATCAATCCGAATACGACGCAGCGAATGACCGATGCGATGGGCCGCTGCGCGCGCG
AAGTCGCGGCGCCCGGCACG

Product: cytosine/purines uracil, thiamine, allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MAQFSVAHDSAYPAQDGGEGGGPALPGGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQ
VLIALIVGISLVNVLCNLIARPSQQIGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPQW
LPYADVHRYGFLGLSAVGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFILAGYMVWRAGWRNIGINLGGVKY
HGAEVIPVMITAISLVVSYFSGPMLNFGDFSRYCRSFGEVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVET
VGRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYTLDVL
GAFIGPLYGVLIADFYLVKRGRLVRDDLYTMSESGTYWYTGGVNRRALAALLPAAVIAIVCVMMPGWQGVANFSWFIGAA
LGFVFYRVLAGTQR

Sequences:

>Translated_494_residues
MAQFSVAHDSAYPAQDGGEGGGPALPGGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQ
VLIALIVGISLVNVLCNLIARPSQQIGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPQW
LPYADVHRYGFLGLSAVGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFILAGYMVWRAGWRNIGINLGGVKY
HGAEVIPVMITAISLVVSYFSGPMLNFGDFSRYCRSFGEVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVET
VGRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYTLDVL
GAFIGPLYGVLIADFYLVKRGRLVRDDLYTMSESGTYWYTGGVNRRALAALLPAAVIAIVCVMMPGWQGVANFSWFIGAA
LGFVFYRVLAGTQR
>Mature_493_residues
AQFSVAHDSAYPAQDGGEGGGPALPGGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQV
LIALIVGISLVNVLCNLIARPSQQIGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPQWL
PYADVHRYGFLGLSAVGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFILAGYMVWRAGWRNIGINLGGVKYH
GAEVIPVMITAISLVVSYFSGPMLNFGDFSRYCRSFGEVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVETV
GRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYTLDVLG
AFIGPLYGVLIADFYLVKRGRLVRDDLYTMSESGTYWYTGGVNRRALAALLPAAVIAIVCVMMPGWQGVANFSWFIGAAL
GFVFYRVLAGTQR

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=469, Percent_Identity=28.997867803838, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6319495, Length=434, Percent_Identity=25.5760368663594, Blast_Score=153, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6322219, Length=439, Percent_Identity=23.9179954441913, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319429, Length=481, Percent_Identity=23.7006237006237, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323266, Length=450, Percent_Identity=22.8888888888889, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324766, Length=452, Percent_Identity=21.9026548672566, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324645, Length=468, Percent_Identity=21.3675213675214, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53611; Mature: 53480

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCC
>Mature Secondary Structure 
AQFSVAHDSAYPAQDGGEGGGPALPGGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVH
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHH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HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]