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The map label for this gene is cspA [H]

Identifier: 161523955

GI number: 161523955

Start: 851690

End: 851893

Strand: Reverse

Name: cspA [H]

Synonym: Bmul_0775

Alternate gene names: 161523955

Gene position: 851893-851690 (Counterclockwise)

Preceding gene: 161523960

Following gene: 161523952

Centisome position: 24.7

GC content: 54.9

Gene sequence:

>204_bases
ATGGCAACTGGTATTGTCAAATGGTTCAACGACGCAAAGGGCTTCGGTTTCATCACTCCCGACGAGGGCGGTGAAGACCT
GTTCGCGCACTTCTCGGCGATCAACATGCAGGGTTTCAAGACCCTGAAGGAAGGCCAGAAGGTGAGCTTCGAAGTCGTCC
AAGGACCGAAGGGCAAGCAAGCTTCGAATATCCAGGCCGCCTGA

Upstream 100 bases:

>100_bases
GAGGTGAGCTTGTGAGGGGGGAACGGCTGCAGTTTTCCGGTCGTTTAGCTTTTCGAGCGTGCTCGTGGTAAGTAGCGACT
GTGTGACAGGGGAAGTAGGT

Downstream 100 bases:

>100_bases
GGGCAACGGATTTCGGACGAAGAAACCCGGCGAGACGCCGGGTTTCTTTTTTTGTGCGTCGCATCTGCGCCGTCTCGATT
GATCGGCGAGTTTGCACAAT

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 66

Protein sequence:

>67_residues
MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQKVSFEVVQGPKGKQASNIQAA

Sequences:

>Translated_67_residues
MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQKVSFEVVQGPKGKQASNIQAA
>Mature_66_residues
ATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQKVSFEVVQGPKGKQASNIQAA

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=65.625, Blast_Score=92, Evalue=6e-21,
Organism=Escherichia coli, GI1786841, Length=63, Percent_Identity=61.9047619047619, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=62.5, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=58.0645161290323, Blast_Score=82, Evalue=8e-18,
Organism=Escherichia coli, GI1788126, Length=63, Percent_Identity=61.9047619047619, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=57.8125, Blast_Score=79, Evalue=7e-17,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=56.4516129032258, Blast_Score=78, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983217, Length=56, Percent_Identity=51.7857142857143, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71983225, Length=56, Percent_Identity=51.7857142857143, Blast_Score=68, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24658520, Length=63, Percent_Identity=44.4444444444444, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7221; Mature: 7090

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQKVSFEVVQGPKGKQ
CCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHCCCCCEEEEEEECCCCCC
ASNIQAA
CCCCCCC
>Mature Secondary Structure 
ATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMQGFKTLKEGQKVSFEVVQGPKGKQ
CCCCEEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHCCCCCEEEEEEECCCCCC
ASNIQAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA