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The map label for this gene is shiA [H]

Identifier: 161523814

GI number: 161523814

Start: 693751

End: 695136

Strand: Reverse

Name: shiA [H]

Synonym: Bmul_0634

Alternate gene names: 161523814

Gene position: 695136-693751 (Counterclockwise)

Preceding gene: 161523816

Following gene: 161523813

Centisome position: 20.16

GC content: 67.39

Gene sequence:

>1386_bases
ATGACCGCACGCGCGGCCCGCGAACCCCAACCCGGACACCAGCCGAAGAAGGCCGCCGCGAGCGGCTGGATCGGCTCGGC
GCTCGAGTACTACGACTTCTTCATCTACGCGACGGCGGCAGCGCTGATCTTTCCGCAGATCTTCTTTCCGAAAGGCGATC
CGACCACCGCGATCGTCGCGTCGCTCGCGACCTACGGCGTCGGCTATGTCGCGCGGCCGATCGGCGCATTCGTGCTCGGC
CACCTCGGCGACACGCACGGTCGCAAGACGGTGCTGCTCTCGTGCATGTTCCTGATGGGCTTCGCGACGATCGGCGTCGG
CCTGCTGCCGACCTACGATCAGATCGGCCTGCTGGCGCCGGTCCTGCTCGTGATCCTGCGGCTGATTCAGGGCTTTGCCG
TAGCCGGCGAAATCTCCGGCGCAAGCTCGATGATCCTCGAACACGCGCCGTTCGGCCGGCGCGGCTTCTTTTCGAGCTTC
ACGCTGCAGGGCGTGCAGGCCGGCCAGGTGCTGGCGGCGGCCGTGTTCCTGCCGCTCGCGCACTACATGCCGACCGAGCA
GTTCAACAGCTGGGGCTGGCGCATTCCGTTCCTGCTGAGCGTCGTGGTGATCTTCGCGGGCTGGGTGATCCGCCGCAACG
TGACGGAGACGCCCGCCTTCACCGGAGAGGAAGCACGGCAGACGCGCGCGCGCTCGCCCGTCGTCGAGGCATTCAGGTTC
CACTGGGCCGACATGCTGCGTGTGGTCTGCATGTCGCTGATGAACGTGATTCCCGTCGTCGCGACGATCTTCGGCGCGGC
CTACGCCGTGCAGCCGGCCTACGGCATCGGATTCGCGAAGGACGTCTACCTCTGGATTCCCGTCGCGGGCAACATCGTCG
CGGTGCTGGTGATTCCGTACGTCGGCAGTTTGTCCGACCGGATCGGGCGCAAGCCGCCGATCGTGTTCGGCGCGCTGCTC
TCGGGGCTGCTCGCCTTCGCTTATCTCTATGCGATCAGCATCCAGAGCGTCCCGCTCGCTTTCGCGATGTCGCTGCTGAT
GTGGGGCGTGGTCTACCAGGGCTACAACGCAGTGTTCCCGAGCTTCTACCCCGAGCTGTTTCCGACGCGCACGCGCGTCT
CCGCGATGGCGATCGCGCAGAACGTCGGTACCGCGATCACCGCGATGCTGCCCGCGCTGTTTACGGCCGTCGCGCCGCCC
GGTGCGACGCACATCTGGCTGACCGTCGGCGCGATCGCGTTCGGCATCACCGTGATCGCCGCGCTGGCCGCGCTCAGCGC
CCGCGAAACCTATCGCATCGCGATGAGCGAGCTGGGCCGCGCCGATGCACGGCCGGTCGACAAGACCGAGTACGATCGCC
GCCGCGCACAGGCGCCGGTGGCGTGA

Upstream 100 bases:

>100_bases
CTTGTCGCGCCGACCTCGCCGCCTCTAACCTTTCAATTTGTAATGAACGCGATCGATGCGTCGATTCGCGCGGAGAAACC
CAACGAAACCGGAGATAGCC

Downstream 100 bases:

>100_bases
GCGCGCCCTTGGCCGGCCATTGGCCGCGCCGCGCCACGCGCGTACAGTAGCGTGCAGCTGCGCCGCCCGGCGGCGCAGGC
CCCGACCGCCCCGACCAAGG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTARAAREPQPGHQPKKAAASGWIGSALEYYDFFIYATAAALIFPQIFFPKGDPTTAIVASLATYGVGYVARPIGAFVLG
HLGDTHGRKTVLLSCMFLMGFATIGVGLLPTYDQIGLLAPVLLVILRLIQGFAVAGEISGASSMILEHAPFGRRGFFSSF
TLQGVQAGQVLAAAVFLPLAHYMPTEQFNSWGWRIPFLLSVVVIFAGWVIRRNVTETPAFTGEEARQTRARSPVVEAFRF
HWADMLRVVCMSLMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVAGNIVAVLVIPYVGSLSDRIGRKPPIVFGALL
SGLLAFAYLYAISIQSVPLAFAMSLLMWGVVYQGYNAVFPSFYPELFPTRTRVSAMAIAQNVGTAITAMLPALFTAVAPP
GATHIWLTVGAIAFGITVIAALAALSARETYRIAMSELGRADARPVDKTEYDRRRAQAPVA

Sequences:

>Translated_461_residues
MTARAAREPQPGHQPKKAAASGWIGSALEYYDFFIYATAAALIFPQIFFPKGDPTTAIVASLATYGVGYVARPIGAFVLG
HLGDTHGRKTVLLSCMFLMGFATIGVGLLPTYDQIGLLAPVLLVILRLIQGFAVAGEISGASSMILEHAPFGRRGFFSSF
TLQGVQAGQVLAAAVFLPLAHYMPTEQFNSWGWRIPFLLSVVVIFAGWVIRRNVTETPAFTGEEARQTRARSPVVEAFRF
HWADMLRVVCMSLMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVAGNIVAVLVIPYVGSLSDRIGRKPPIVFGALL
SGLLAFAYLYAISIQSVPLAFAMSLLMWGVVYQGYNAVFPSFYPELFPTRTRVSAMAIAQNVGTAITAMLPALFTAVAPP
GATHIWLTVGAIAFGITVIAALAALSARETYRIAMSELGRADARPVDKTEYDRRRAQAPVA
>Mature_460_residues
TARAAREPQPGHQPKKAAASGWIGSALEYYDFFIYATAAALIFPQIFFPKGDPTTAIVASLATYGVGYVARPIGAFVLGH
LGDTHGRKTVLLSCMFLMGFATIGVGLLPTYDQIGLLAPVLLVILRLIQGFAVAGEISGASSMILEHAPFGRRGFFSSFT
LQGVQAGQVLAAAVFLPLAHYMPTEQFNSWGWRIPFLLSVVVIFAGWVIRRNVTETPAFTGEEARQTRARSPVVEAFRFH
WADMLRVVCMSLMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVAGNIVAVLVIPYVGSLSDRIGRKPPIVFGALLS
GLLAFAYLYAISIQSVPLAFAMSLLMWGVVYQGYNAVFPSFYPELFPTRTRVSAMAIAQNVGTAITAMLPALFTAVAPPG
ATHIWLTVGAIAFGITVIAALAALSARETYRIAMSELGRADARPVDKTEYDRRRAQAPVA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=31.042654028436, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790550, Length=387, Percent_Identity=33.0749354005168, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1789941, Length=431, Percent_Identity=33.8747099767981, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1788942, Length=363, Percent_Identity=30.8539944903581, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87082404, Length=432, Percent_Identity=21.9907407407407, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082231, Length=331, Percent_Identity=24.773413897281, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49622; Mature: 49491

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TARAAREPQPGHQPKKAAASGWIGSALEYYDFFIYATAAALIFPQIFFPKGDPTTAIVA
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH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HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]