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The map label for this gene is dmlR [H]

Identifier: 161523786

GI number: 161523786

Start: 664587

End: 665519

Strand: Reverse

Name: dmlR [H]

Synonym: Bmul_0606

Alternate gene names: 161523786

Gene position: 665519-664587 (Counterclockwise)

Preceding gene: 161523790

Following gene: 161523785

Centisome position: 19.3

GC content: 68.81

Gene sequence:

>933_bases
ATGGATCGACTCGGCGACATCCGGCTGTTCGTGGAAGCGGCCGAACTCGGCAGCCTGTCGGCGGCCGGACGCAAGCTGAA
CCTGACGCCGGCGGCGGCCAGCGCGCGACTCGCGAAGCTCGAGGCGAAAATCGCGACGCGGCTGTTCGAGCGTTCGACGC
GGCAGTTGCGGCTCACCGACGAAGGGCGGCTGTACCTCAACTGCTGCCGGCAGGCGCTGCAGGCGCTCGACGACGCCGAC
GCGATGCTGCAGGAGGGCCGCAATGTCGTCGGCGGCAAGGTGCGGCTGTCGTCGACGTCCGATTTCGGCCGCAACCAGCT
GCTCGACTGGCTCGACGAATTCACCGCGCGGCATCCCGACGTCACGTTCTCGCTGACGGCGTCCGATTCGTCGTCGAATC
TCTGGCAGGACGAGATCGACCTCGCGATCCGCTTCGCGGCGCCGCCCGACGGCGCGCTGATCGCGCGGCGGCTCGCCGCG
AACCGGCGCGTGCTGTGCGCGGCGCCGTCGTTCATCGAACGCTACGGAACGCCCGTCGACCCGAACGATCTGGCCCGCTT
TCCTTGCAACGTGATCACGATTGCATCGGGGCCGATGAACACCTGGCGCTTCACACGCGGCGACGAGGTGCAGACCTACA
CGGTGCCGATGTCGAACGCGTTCGAGACCAACGACGGCGGCCTCACGCGCGAATGGACGCTGCGCGGGCACGGCATCGCG
CTGAAGTCGCTGTGGGACATCGCCGACGACGTGCGCGCAGGCCGCCTGCGCGTGCTGCTGCCCGAATGGCGCCACCAGGA
CGCGCCGCTGCACGCGATCTATCACAGCAAGCGCTACATGGCGCCGCGCGTGCGCGTGCTGCTCGACTTTCTCGCCGAGC
GCTTTGCGCGCGAGGAAGCGGCGCTCGAGGATCTGCTGAACGCATGCCGGTGA

Upstream 100 bases:

>100_bases
GCCGCATCGCACAAGTTCGTGCGCGCATTGTCCCGAACGCGCCGATATTTGATAATTGGCGCGACGTTTGAACCATTTTC
AAAAGATTCTGAAAAATCGC

Downstream 100 bases:

>100_bases
TGGCCCTGCGTGCGGATGCGCGAAGGGTCGGAACGCCAATCCGGGTGTCGCGCGGCCACCACCGGGCGGCCGCCTCGGCC
CGCGCGAAAAGCTATAATGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKIATRLFERSTRQLRLTDEGRLYLNCCRQALQALDDAD
AMLQEGRNVVGGKVRLSSTSDFGRNQLLDWLDEFTARHPDVTFSLTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAA
NRRVLCAAPSFIERYGTPVDPNDLARFPCNVITIASGPMNTWRFTRGDEVQTYTVPMSNAFETNDGGLTREWTLRGHGIA
LKSLWDIADDVRAGRLRVLLPEWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEAALEDLLNACR

Sequences:

>Translated_310_residues
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKIATRLFERSTRQLRLTDEGRLYLNCCRQALQALDDAD
AMLQEGRNVVGGKVRLSSTSDFGRNQLLDWLDEFTARHPDVTFSLTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAA
NRRVLCAAPSFIERYGTPVDPNDLARFPCNVITIASGPMNTWRFTRGDEVQTYTVPMSNAFETNDGGLTREWTLRGHGIA
LKSLWDIADDVRAGRLRVLLPEWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEAALEDLLNACR
>Mature_310_residues
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKIATRLFERSTRQLRLTDEGRLYLNCCRQALQALDDAD
AMLQEGRNVVGGKVRLSSTSDFGRNQLLDWLDEFTARHPDVTFSLTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAA
NRRVLCAAPSFIERYGTPVDPNDLARFPCNVITIASGPMNTWRFTRGDEVQTYTVPMSNAFETNDGGLTREWTLRGHGIA
LKSLWDIADDVRAGRLRVLLPEWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEAALEDLLNACR

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=31.864406779661, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1789639, Length=305, Percent_Identity=28.8524590163934, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=31.3793103448276, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=32.8719723183391, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=29.2517006802721, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=27.3972602739726, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=25.8823529411765, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788706, Length=174, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=30.0699300699301, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=24.4094488188976, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790011, Length=298, Percent_Identity=22.8187919463087, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=25, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34709; Mature: 34709

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKIATRLFERSTRQLRLTD
CCCCCCEEEEEEHHHHCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
EGRLYLNCCRQALQALDDADAMLQEGRNVVGGKVRLSSTSDFGRNQLLDWLDEFTARHPD
CCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCC
VTFSLTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAANRRVLCAAPSFIERYGTPVD
EEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECHHHHHHCCCCCC
PNDLARFPCNVITIASGPMNTWRFTRGDEVQTYTVPMSNAFETNDGGLTREWTLRGHGIA
CCHHHHCCCCEEEEECCCCCCCEECCCCCEEEEEECCCCCEECCCCCCEEEEEECCCCEE
LKSLWDIADDVRAGRLRVLLPEWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEA
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
ALEDLLNACR
HHHHHHHHCC
>Mature Secondary Structure
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAKIATRLFERSTRQLRLTD
CCCCCCEEEEEEHHHHCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
EGRLYLNCCRQALQALDDADAMLQEGRNVVGGKVRLSSTSDFGRNQLLDWLDEFTARHPD
CCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCC
VTFSLTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAANRRVLCAAPSFIERYGTPVD
EEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECHHHHHHCCCCCC
PNDLARFPCNVITIASGPMNTWRFTRGDEVQTYTVPMSNAFETNDGGLTREWTLRGHGIA
CCHHHHCCCCEEEEECCCCCCCEECCCCCEEEEEECCCCCEECCCCCCEEEEEECCCCEE
LKSLWDIADDVRAGRLRVLLPEWRHQDAPLHAIYHSKRYMAPRVRVLLDFLAERFAREEA
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
ALEDLLNACR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]